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Chitinophaga aurantiaca sp. nov., isolated from a soil sample from a tangerine field

  • Su-Kyung Kim
  • MooChang Kook
  • Zheng-Fei Yan
  • Sang-Yong Park
  • Sang Suk Kim
  • Ho Bin Kim
  • Huan Trinh
  • Kyung Hwa Won
  • Jung-Eun Yang
  • Tae-Hoo YiEmail author
Original Paper
  • 22 Downloads

Abstract

A Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated THG-SD5.5T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Chitinophaga and to be closely related to Chitinophaga ginsengihumi KACC 17604T (97.9%) and Chitinophaga rupis KACC 14521T (97.5%). The 16S rRNA gene sequence similarities with other species of the genus Chitinophaga were in the range 92.8–95.5%. Catalase test was positive. Oxidase test was negative. The DNA G + C content was determined to be 46.1 mol%. DNA-DNA hybridization values between strain THG-SD5.5T and C. ginsengihumi KACC 17604T and C. rupis KACC 14521T were 45.1% and 15.6%, respectively. Strain THG-SD5.5T was also found to be able to grow at 24–33 °C, at 0–5% NaCl and at pH 5.5–9.0. The major fatty acids were identified as anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. The dominant respiratory quinone was identified as menaquinone-7 (MK-7). The polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and three unidentified lipids. Based on these phenotypic, genotypic and phylogenetic characterisations, strain THG-SD5.5T (= KACC 19338T = CGMCC 1.16304T) is concluded to represent a novel species of the genus Chitinophaga, for which the name Chitinophaga aurantiaca sp. nov. is proposed.

Keywords

Chitinophaga aurantiaca sp. nov. Taxonomy Tangerine field THG-SD5.5 

Notes

Funding

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012655042018)” Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Ethical standards

This is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carry out the data analysis and manuscript writing and “This article does not contain any studies with human participants or animals performed by any of the authors”. Moreover, all authors read and approved the final manuscript.

Supplementary material

10482_2019_1251_MOESM1_ESM.pptx (760 kb)
Supplementary material 1 (PPTX 759 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Su-Kyung Kim
    • 1
  • MooChang Kook
    • 2
  • Zheng-Fei Yan
    • 1
  • Sang-Yong Park
    • 3
  • Sang Suk Kim
    • 4
  • Ho Bin Kim
    • 5
  • Huan Trinh
    • 1
  • Kyung Hwa Won
    • 3
  • Jung-Eun Yang
    • 3
  • Tae-Hoo Yi
    • 1
    Email author
  1. 1.Department of Oriental Medicinal Material and Processing, College of Life ScienceKyung Hee University Global CampusYongin-siRepublic of Korea
  2. 2.Department of Food and NutritionBaewha Women’s UniversitySeoulRepublic of Korea
  3. 3.Seoul Hightech Venture CenterSD Biotechnologies Co., Ltd.SeoulRepublic of Korea
  4. 4.Citrus Research InstituteNational Institute of Horticultural & Herbal Science, RDAJejuKorea
  5. 5.Woongjin Foods R&D CenterSeoulKorea

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