Transcription factor CgAzf1 regulates melanin production, conidial development and infection in Colletotrichum gloeosporioides

  • Xiaoyu Li
  • Zhijian Ke
  • Xinjun Yu
  • Zhiqiang LiuEmail author
  • Chenghui Zhang
Original Paper


Rubber anthracnose caused by Colletotrichum gloeosporioides leads to huge economic loss in the natural rubber industry every year. Conidia of C. gloeosporioides are a major infection source but little is known about molecular mechanisms underlying conidial development and infection. In this study, the C. gloeosporioide C2H2 zinc-finger protein transcription factor gene CgAzf1 is shown to be involved in melanin production, conidial development and infection. Deletion of CgAzf1 resulted in decreased melanin production and hydrophilicity of aerial mycelium was increased. The mutants also showed reduced conidiation, low germination rate, and the formation of appressorium lagged too. Virulence assays showed that the CgAzf1 deletion strain could not infect intact rubber tree leaves and had an attenuated virulence on the wounded leaves. Quantitative RT-PCR showed that CgAzf1 regulates expression of genes involved in the MAPK, cAMP-PKA and melanin biosynthesis pathways.


Colletotrichum gloeosporioides Rubber anthracnose Transcription factor Conidium Pathogenicity 



This research was supported by the National Natural Science Foundation of China (Grant Nos. 31860480 and 31560045).

Conflict of interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University)Ministry of EducationHaikouChina
  2. 2.Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and BioengineeringZhejiang University of TechnologyHangzhouChina

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