Advertisement

Transcription factor CgAzf1 regulates melanin production, conidial development and infection in Colletotrichum gloeosporioides

  • Xiaoyu Li
  • Zhijian Ke
  • Xinjun Yu
  • Zhiqiang LiuEmail author
  • Chenghui Zhang
Original Paper
  • 5 Downloads

Abstract

Rubber anthracnose caused by Colletotrichum gloeosporioides leads to huge economic loss in the natural rubber industry every year. Conidia of C. gloeosporioides are a major infection source but little is known about molecular mechanisms underlying conidial development and infection. In this study, the C. gloeosporioide C2H2 zinc-finger protein transcription factor gene CgAzf1 is shown to be involved in melanin production, conidial development and infection. Deletion of CgAzf1 resulted in decreased melanin production and hydrophilicity of aerial mycelium was increased. The mutants also showed reduced conidiation, low germination rate, and the formation of appressorium lagged too. Virulence assays showed that the CgAzf1 deletion strain could not infect intact rubber tree leaves and had an attenuated virulence on the wounded leaves. Quantitative RT-PCR showed that CgAzf1 regulates expression of genes involved in the MAPK, cAMP-PKA and melanin biosynthesis pathways.

Keywords

Colletotrichum gloeosporioides Rubber anthracnose Transcription factor Conidium Pathogenicity 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant Nos. 31860480 and 31560045).

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Adachi K, Hamer JE (1998) Divergent cAMP signaling pathways regulate growth and pathogenensis in the rice blast fungus Magnaporthe grisea. Plant Cell 10:1361–1373CrossRefGoogle Scholar
  2. Bhadauria V, Wang LX, Peng YL (2010) Proteomic changes associated with deletion of the Magnaporthe oryzae conidial morphology-regulating gene COM1. Biol Direct 5:61CrossRefGoogle Scholar
  3. Gomes S, Prieto P, Martins-Lopes P, Carvalho T, Martin A, Guedes-Pinto H (2009) Development of Colletotrichum acutatum on tolerant and susceptible Olea europaea L. cultivars: a microscopic analysis. Mycopathologia 168:203–211CrossRefGoogle Scholar
  4. Jean G, Edith NO, Fabrice P (2005) Some epidemiological investigations on Colletotrichum leaf disease on rubber tree. Crop Prot 24:65–77CrossRefGoogle Scholar
  5. Li X, Han X, Liu Z, He C (2013) The function and properties of the transcriptional regulator COS1 in Magnaporthe oryzae. Fungal Biol 117:239–249CrossRefGoogle Scholar
  6. Li X, Wu Y, Liu Z, Zhang C (2017) The function and transcriptome analysis of a bZIP transcription factor CgAP1 in Colletotrichum gloeosporioides. Microbiol Res 197:39–48CrossRefGoogle Scholar
  7. Liu Z, Wu M, Ke Z, Liu W, Li X (2018) Functional analysis of a regulator of G-protein signaling CgRGS1 in the rubber tree anthracnose fungus Colletotrichum gloeosporioides. Arch Microbiol 200:391–400CrossRefGoogle Scholar
  8. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆CT method. Methods 25:402–408CrossRefGoogle Scholar
  9. Pareek M, Rajam MV (2017) RNAi-mediated silencing of MAP kinase signalling genes (Fmk1, Hog1, and Pbs2) in Fusarium oxysporum reduces pathogenesis on tomato plants. Fungal Biol 121:775–784CrossRefGoogle Scholar
  10. Priyatno TP, Abu Bakar FD, Kamaruddin N, Mahadi NM, Abdul Murad AM (2012) Inactivation of the catalytic subunit of cAMP-dependent protein kinase A causes delayed appressorium formation and reduced pathogenicity of Colletotrichum gloeosporioides. Sci World J 2012:545784CrossRefGoogle Scholar
  11. Prusky D, Lichter A (2008) Mechanisms modulating fungal attack in post-harvest pathogen interactions and their control. Eur J Plant Pathol 121:281–289CrossRefGoogle Scholar
  12. Shelp BJ, Bown AW, McLean MD (1999) Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci 4:446–452CrossRefGoogle Scholar
  13. Slattery MG, Liko D, Heideman W (2006) The function and properties of the Azf1 transcriptional regulator change with growth conditions in Saccharomyces cerevisiae. Eukaryot Cell 5:313–320CrossRefGoogle Scholar
  14. Talbot NJ, Ebbole DJ, Hamer JE (1993) Identification and characterization of MPGI, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5:1575–1590CrossRefGoogle Scholar
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar
  16. Tsuji G, Kenmochi Y, Takano Y, Sweigard J, Farrall L, Furusawa I, Horino O, Kubo Y (2000) Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn(II)2Cys6 binuclear cluster DNA-binding motifs and regulate transcription of melanin biosynthesis genes in a developmentally specific manner. Mol Microbiol 38:940–954CrossRefGoogle Scholar
  17. Wang X, Xu X, Liang Y, Wang Y, Tian C (2018) A Cdc42 homolog in Colletotrichum gloeosporioides regulates morphological development and is required for ROS-mediated plant infection. Curr Genet 64:1153–1169CrossRefGoogle Scholar
  18. Wösten HA (2001) Hydrophobins: multipurpose proteins. Annu Rev Microbiol 55:625–646CrossRefGoogle Scholar
  19. Wösten HA, Schuren FH, Wessels JG (1994) Interfacial self-assembly of a hydrophobin into an amphipathic protein membrane mediates fungal attachment to hydrophobic surfaces. EMBO J 13:5848–5854CrossRefGoogle Scholar
  20. Xu JR, Urban M, Sweigard JA, Hamer JE (1997) The CPKA gene of Magnaporthe grisea is essential for appressorial penetration. Mol Plant Microbe Interact 10:187–194CrossRefGoogle Scholar
  21. Zheng W, Zhao Z, Chen J, Liu W, Ke H, Zhou J, Lu G, Darvill AG, Albersheim P, Wu S, Wang Z (2009) A Cdc42 ortholog is required for penetration and virulence of Magnaporthe grisea. Fungal Genet Biol 46:450–460CrossRefGoogle Scholar
  22. Zhou ZZ, Li GH, Lin CH, He CZ (2009) Conidiophore Stalk-less1 encodes a putative zinc-finger protein involved in the early stage of conidiation and mycelial infection in Magnaporthe oryzae. Mol Plant Microbe Interact 22:402–410CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University)Ministry of EducationHaikouChina
  2. 2.Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and BioengineeringZhejiang University of TechnologyHangzhouChina

Personalised recommendations