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Pontibacter chitinilyticus sp. nov., a novel chitin-hydrolysing bacterium isolated from soil

  • Geeta Chhetri
  • Jiyoun Kim
  • Inhyup Kim
  • Myung Kyum Kim
  • Taegun SeoEmail author
Original Paper
  • 16 Downloads

Abstract

A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women’s University field. The strain can grow at 7–37 °C, pH 6.0–8.0, and can tolerate up to 5.5% (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter. The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B). The predominant menaquinone was identified as MK-7. The DNA G+C content was determined to be 48.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp. nov. is proposed. The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).

Keywords

Pontibacter chitinilyticus sp. nov Chitin hydrolysis Polyphasic taxonomy Cytophagaceae Soil bacteria 

Notes

Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and the Project on Survey of Indigenous Species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) (NIBR201701206). We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for the suggested species name.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical standard

This study does not describe any experimental work related to human.

Supplementary material

10482_2019_1235_MOESM1_ESM.docx (1.6 mb)
Supplementary material 1 (DOCX 1615 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Geeta Chhetri
    • 1
  • Jiyoun Kim
    • 1
  • Inhyup Kim
    • 1
  • Myung Kyum Kim
    • 2
  • Taegun Seo
    • 1
    Email author
  1. 1.Department of Life ScienceDongguk University-SeoulGoyangSouth Korea
  2. 2.Department of Bio and Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulSouth Korea

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