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Cohnella candidum sp. nov., radiation-resistant bacterium from soil

  • SooHyun Maeng
  • Myung Kyum Kim
  • Jun Hwee Jang
  • Hana Yi
  • Gayathri SubramaniEmail author
Original Paper

Abstract

A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7T, was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%). Optimal growth occurred at 30 °C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C15:0 and iso-C16:0. The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G + C content was 57.0 mol %. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7T from all recognized Cohenella species. The strain 18JY8-7T, therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7T (= KCTC 33969T = JCM 33199T).

Keywords

Paenibacillaceae Cohnella Novel species Taxonomy Radiation-resistant 

Notes

Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and by a Grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201801106).

Author’s contributions

All authors equally contributed in this work.

Conflict of interest

All authors certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

Supplementary material

10482_2019_1233_MOESM1_ESM.pptx (43 kb)
Supplementary material 1 (PPTX 43 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • SooHyun Maeng
    • 1
  • Myung Kyum Kim
    • 2
  • Jun Hwee Jang
    • 3
  • Hana Yi
    • 1
    • 4
  • Gayathri Subramani
    • 2
    Email author
  1. 1.Department of Public Health Sciences, Graduate SchoolKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Bio and Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulRepublic of Korea
  3. 3.Department of Horticultural Science, School of Applied Biosciences, Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea
  4. 4.School of Biosystem and Biomedical ScienceKorea UniversitySeoulRepublic of Korea

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