Production of a novel killer toxin from Saccharomyces eubayanus using agro-industrial waste and its application against wine spoilage yeasts

  • María Belén Mazzucco
  • María Angélica Ganga
  • Marcela Paula SangorrínEmail author
Original Paper


The juicing industry generates large amounts of waste that mostly lack commercial value and, in the absence of waste treatment policies, produces environmental pollution. Also, microbiological spoilage is a major concern in the wine industry and control tools are limited. Taking these challenges into account, agro-industrial waste coming from ultrafiltrated apple and pear juice were used to grow Saccharomyces eubayanus and to produce its killer toxin (SeKT). A Plackett–Burman screening was performed in order to optimize SeKT production in ultrafiltrated apple and pear juice. The optimized medium was characterized: 75% v/v WUJ, 0.5% m/v KH2PO4, 0.5% m/v MgSO4, 0.5% m/v (NH4)SO4, 0.5% g/L urea, 10% v/v glycerol and 0.1% v/v Triton X-100. SeKT produced in WUJ optimised medium was used to perform killer assays against wine spoilage yeasts and showed antagonistic activity against Brettanomyces bruxellensis, Pichia guilliermondii, Pichia manshurica and Pichia membranifaciens. Different inhibition percentages against spoilage species in a wine environment (49–69%) were detected and preserved for at least 48 h. For the first time, this work reports the ability of S. eubayanus to produce a killer toxin with potential use as a biocontrol tool in winemaking. Producing SeKT using agro-industrial waste as an alternative medium to cultivate S. eubayanus would have industrial, economic and ecological benefits.


Agro-industrial waste Biocontrol Killer toxins Saccharomyces eubayanus Spoilage yeast 



This work was supported by Universidad Nacional del Comahue, ANPCyT PICT 2780 and CONICET PUE0067. B. Mazzucco is a recipient of a fellowship from CONICET. M. A. Ganga gives thanks to Basal Grant Usach USA1398_GM181622.

Author contributions

Dra. MPS designed the study and corrected the manuscript. Dra. MAG analysed data and contributed new methods. Dra. MBM carried out the experimental work and wrote the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study. Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities—tacitly or explicitly—at the PROBIEN Institute where the work has been carried out, before the work is submitted.

Supplementary material

10482_2019_1231_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 11 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Instituto de Investigación y Desarrollo en Ingeniería de ProcesosBiotecnología y Energías Alternativas (PROBIEN, Consejo Nacional de Investigaciones Científicas y Tecnológicas - Universidad Nacional del Comahue)NeuquénArgentina
  2. 2.Laboratorio de Biotecnología y Microbiología Aplicada, Departamento en Ciencia y Tecnología de los Alimentos, Facultad TecnológicaUniversidad de Santiago de ChileEstación CentralChile

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