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Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection

  • Leonardo Ribeiro Bastos
  • Maria Clara Fernandes Martins
  • Rodolpho Mattos Albano
  • Elizabeth Andrade Marques
  • Robson Souza LeãoEmail author
Short Communication

Abstract

We present a post-operative infection caused by a methicillin-resistant Staphylococcus aureus strain, previously isolated in the preoperative screening, in a patient submitted to femoral osteosynthesis, successfully treated with oral ciprofloxacin. The isolate exhibited in vitro resistance to ciprofloxacin, Staphylococcal Cassette Chromosome mec type IV, it was negative for the lukS-PV Panton–Valentine leucocidin gene and belonged to ST2594 in multilocus sequence typing analysis. Whole genome sequencing revealed a genome size of 2,818,289 base pairs. The annotated genomes of ST2594 and N315 strains were compared, looking for genes related to virulence and resistance. The lack of the tst, sec, sel genes, associated with a mutation in the clfA gene, may partially explain the low morbity in this case.

Keywords

Bacterial genome Carrier state Methicillin-resistant Staphylococcus aureus Surgical wound infection 

Notes

Funding

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq.).

Compliance with ethical sandards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee (CAAE: 39070314.0.0000.5256).

Informed consent

Informed consent was obtained from the participant included in the study.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Leonardo Ribeiro Bastos
    • 1
  • Maria Clara Fernandes Martins
    • 2
  • Rodolpho Mattos Albano
    • 3
  • Elizabeth Andrade Marques
    • 1
  • Robson Souza Leão
    • 1
    Email author
  1. 1.Department of Microbiology, Immunology and Parasitology, Medical Science SchoolState University of Rio de JaneiroRio de JaneiroBrazil
  2. 2.Institute of BioscienceFederal University of Rio de JaneiroRio de JaneiroBrazil
  3. 3.Biochemistry Department, Institute of Biology Roberto Alcântara GomesState University of Rio de JaneiroRio de JaneiroBrazil

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