Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection

  • Leonardo Ribeiro Bastos
  • Maria Clara Fernandes Martins
  • Rodolpho Mattos Albano
  • Elizabeth Andrade Marques
  • Robson Souza LeãoEmail author
Short Communication


We present a post-operative infection caused by a methicillin-resistant Staphylococcus aureus strain, previously isolated in the preoperative screening, in a patient submitted to femoral osteosynthesis, successfully treated with oral ciprofloxacin. The isolate exhibited in vitro resistance to ciprofloxacin, Staphylococcal Cassette Chromosome mec type IV, it was negative for the lukS-PV Panton–Valentine leucocidin gene and belonged to ST2594 in multilocus sequence typing analysis. Whole genome sequencing revealed a genome size of 2,818,289 base pairs. The annotated genomes of ST2594 and N315 strains were compared, looking for genes related to virulence and resistance. The lack of the tst, sec, sel genes, associated with a mutation in the clfA gene, may partially explain the low morbity in this case.


Bacterial genome Carrier state Methicillin-resistant Staphylococcus aureus Surgical wound infection 



Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq.).

Compliance with ethical sandards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee (CAAE: 39070314.0.0000.5256).

Informed consent

Informed consent was obtained from the participant included in the study.


  1. Aamot HV et al (2012) Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients. Eur J Clin Microbiol Infect Dis 31(8):1999–2004CrossRefGoogle Scholar
  2. Al-Talib H et al (2009) A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton–Valentine Leucocidin. BMC Microbiol 9(1): 113.
  3. Anderson AS et al (2016) Staphylococcus aureus clumping factor a remains a viable vaccine target for prevention of S. Aureus infect. MBio 7(2):e00225–e00316CrossRefGoogle Scholar
  4. Boye K et al (2007) A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCC mec types I-V. Clin Microbiol Infect 13(7):725–727CrossRefGoogle Scholar
  5. Claes J et al (2017) Clumping factor A, von Willebrand factor-binding protein and von Willebrand factor anchor Staphylococcus aureus to the vessel wall. J Thromb Haemost 15(5):1009–1019CrossRefGoogle Scholar
  6. Darling ACE et al (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403CrossRefGoogle Scholar
  7. Dastgheyb S et al (2015) Effect of biofilms on recalcitrance of Staphylococcal joint infection to antibiotic treatment. J Infect Dis 211:641–650CrossRefGoogle Scholar
  8. EUCAST (2017) The european committee on antimicrobial susceptibility testing. Clinical breakpoints (Bacterial v7.1). Accessed 16 Aug 2017
  9. Higgins J et al (2006) Clumping factor A of Staphylococcus aureus inhibits phagocytosis by human polymorphonuclear leucocytes. FEMS Microbiol Lett 258(2):290–296CrossRefGoogle Scholar
  10. Josefsson E et al (2008) In vivo sortase A and clumping factor A mRNA expression during Staphylococcus aureus infection. Microb Pathog 44(2):103–110CrossRefGoogle Scholar
  11. Pires FV et al 2014 Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: a population-based survey. PLoS ONE, 9(3), p.e92537. Available at:
  12. Ryu S et al (2014) Colonization and infection of the skin by S. aureus: immune system evasion and the response to cationic Antimicrobial peptides. Int J Mol Sci 15(5):8753–8772CrossRefGoogle Scholar
  13. Vergara A et al (2017) Biofilm formation and its relationship with the molecular characteristics of food-related Methicillin-resistant Staphylococcus aureus (MRSA). J Food Sci 82(10):2364–2370CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Leonardo Ribeiro Bastos
    • 1
  • Maria Clara Fernandes Martins
    • 2
  • Rodolpho Mattos Albano
    • 3
  • Elizabeth Andrade Marques
    • 1
  • Robson Souza Leão
    • 1
    Email author
  1. 1.Department of Microbiology, Immunology and Parasitology, Medical Science SchoolState University of Rio de JaneiroRio de JaneiroBrazil
  2. 2.Institute of BioscienceFederal University of Rio de JaneiroRio de JaneiroBrazil
  3. 3.Biochemistry Department, Institute of Biology Roberto Alcântara GomesState University of Rio de JaneiroRio de JaneiroBrazil

Personalised recommendations