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Notoacmeibacter ruber sp. nov., a novel endophytic bacterium isolated from leaf of Rhizophora stylosa

  • Xiao-Rui Yan
  • Li TuoEmail author
Original Paper

Abstract

A Gram-stain negative, aerobic, coccus-shaped bacterium, designated strain M2BS9Y-3-1T, was isolated from a surface-sterilized leaf of Rhizophora stylosa. It grew in the pH range 5.0–9.0 (optimum, 7.0) and at temperatures between 20 and 37 °C (optimum, 30 °C). Growth was possible at concentrations of 1–10% (w/v) NaCl (optimum, 2%). The phylogenetic trees based on 16S rRNA gene sequences indicated that strain M2BS9Y-3-1T clustered together with Notoacmeibacter marinus XMTR2A4T (98.44% similarity) and separated from other species of the family Notoacmeibacteraceae. The average nucleotide identity and in silico DNA–DNA hybridization values between strain M2BS9Y-3-1T and N. marinus XMTR2A4T were 76.8%, 19.8%, respectively. The G+C content of the genomic DNA was 60.0 mol%. The cell wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone. The major fatty acid was C18:1ω7c. The lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid and three unidentified lipids. The results of taxonomic analysis suggested that strain M2BS9Y-3-1T represents a novel species of the genus Notoacmeibacter for which the name Notoacmeibacter ruber sp. nov. is proposed. The type strain is M2BS9Y-3-1T (KCTC 62838T = CGMCC 1.13746T).

Keywords

Notoacmeibacter Endophytic bacterium Polyphasic approach Novel species 

Notes

Acknowledgements

This research was supported by the National Natural Sciences Foundation of China (NSFC, Grant No. 81603079) and Youth Science and technology personnal growth Project of Guizhou Provincial Education Department (No. QianJiaoHe KY Zi[2016]200).

Author contributions

XRY has made substantial contributions to data acquisition, experimental operation and manuscript preparation. LT conceived and designed the experiments and edited the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants and/or animals performed by any of the authors.

Supplementary material

10482_2019_1225_MOESM1_ESM.pdf (275 kb)
Supplementary material 1 (PDF 274 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Research Center for Medicine and BiologyZunyi Medical UniversityZunyiPeople’s Republic of China
  2. 2.Zunyi Engineering Research Center of Physical Testing and Chemical AnalysisZunyi Medical UniversityZunyiPeople’s Republic of China

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