Winogradskyella algae sp. nov., a marine bacterium isolated from the brown alga
- 90 Downloads
An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium Kr9-9T was isolated from a brown alga specimen collected near the Kuril Islands. Based on the 16S rRNA gene sequence analysis strain Kr9-9T was assigned to the genus Winogradskyella, and its close phylogenetic neighbors were found to be Winogradskyella damuponensis KCTC 23552T, Winogradskyella sediminis LMG 28075T, and Winogradskyella rapida CCUG 59098T showing high similarities of 98.1%, 97.5%, and 97.1%, respectively. It contained MK-6 as the predominant menaquinone and iso-C15:0, anteiso-C15:0, iso-C16:0 3-OH followed by iso-C15:1 as the major fatty acids. Polar lipids included phosphatidylethanolamine, three unidentified aminolipids and an unidentified lipid. The DNA C+C content was 32.3 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Kr9-9T is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella algae sp. nov. is proposed. The type strain of the species is strain Kr9-9T (= KMM 8180T = KACC 19709T).
KeywordsWinogradskyella algae sp. nov. Marine Brown alga
We would like to thank Dr. Claudine Vereecke, BCCM™/LMG Bacteria Collection, Ghent University, Gent, Belgium, for providing the type strain Winogradskyella sediminis LMG 28075T for comparative analyses. This study was supported by a programme of “Bioresource collections”.
Author V.V. Kurilenko isolated novel strain, designed study and tested bacteria. Author L.A. Romanenko tested bacteria, analyzed data and wrote the first draft of the manuscript. Authors V.V. Kurilenko and M.P. Isaeva performed the molecular studies and literature searches. Author V.I. Svetashev carried out lipid analyses. Author V.V. Mikhailov managed the analyses of the study and analyzed the results. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
All the authors have declared no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
- Nedashkovskaya OI, Kim SB, Han SK, Snauwaert C, Vancanneyt M, Swings J, Kim KO, Lysenko AM, Rohde M, Frolova GM, Mikhailov VV, Bae KS (2005) Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55:49–55CrossRefPubMedGoogle Scholar
- Nedashkovskaya OI, Vancanneyt M, Kim SB, Zhukova NV (2009) Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 59:465–1468Google Scholar
- Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV (2012) Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int J Syst Evol Microbiol 62:1450–1456CrossRefPubMedGoogle Scholar
- Romanenko LA, Tanaka N, Frolova GM, Mikhailov VV (2009) Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int J Syst Evol Microbiol 59:143–1446Google Scholar
- Sasser M (1990) Microbial identification by gas chromatographic analysis of fatty acid methyl esters (GC-FAME). Technical Note 101. MIDI, DE, NewarkGoogle Scholar
- Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microb Today 45:153–155Google Scholar