Nevskia lacus sp. nov., a gammaproteobacterium isolated from a eutrophic lake

  • Yingshun Cui
  • Seong-Jun Chun
  • A-Ra Cho
  • Shu Kuan Wong
  • Hyung-Gwan Lee
  • Hee-Mock OhEmail author
  • Chi-Yong AhnEmail author
Original Paper


A novel Gram-stain negative, rod-shaped and motile bacterial strain, designated strain Seoho-38T, was isolated from a eutrophic lake in South Korea. Polyphasic taxonomic studies were performed to investigate the taxonomic position of the new isolate. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain Seoho-38T formed a distinct cluster with Nevskia ramosa Soe1T, Nevskia persephonica G6M-30T, Nevskia soli GR15-1T, Nevskia terrae KIS13-15T and Nevskia aquatilis F2-63T with bootstrap resampling value of 100%. Of those Nevskia strains, the new isolate shows high sequence similarity with N. ramosa Soe1T (98.7%) and N. persephonica G6M-30T (97.2%), and values lower than 96.5% with the other type strains. The new isolate was observed to grow aerobically in 0–1.5% (w/v) NaCl (optimum 0%), at pH 7.0–9.0 (optimum pH 7.0) and temperature 15–36 °C (optimum 20–30 °C) on R2A medium. DNA–DNA relatedness values between strain Seoho-38T and the type strains of reference species in the genus Nevskia were < 24%. The genomic DNA G + C content was determined to be 67.4 mol%. Ubiquinone-8 (Q-8) (95%) and ubiquinone-7 (Q-7) (5%) were identified as the respiratory quinones. The cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phosphoaminolipid, two glycolipids, an aminolipid and four unidentified lipids. The major fatty acid components were found to include summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:0 ω7c and/or C18:0 ω6c), C16:0 and C14:0. Based on the above polyphasic evidence, strain Seoho-38T (= KCTC 52221T = JCM 31888T) represents a new species of the genus Nevskia, for which the name Nevskia lacus sp. nov. is proposed.


Nevskia lacus sp. nov. Nevskia Polyphasic taxonomy Harmful cyanobacterial blooms 



This research was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) and Korea Research Fellowship program, funded by the Ministry of Science and ICT (2016M1A5A1027453 and 2015H1D3A1060001).

Authors contribution

Y.C. analysed data, interpreted results and wrote the manuscript; S.J.C. and C.Y.A took samples; Y.C. and S.J.C. performed genetic, morphological, physiological and chemotaxonomical analysis. A.R.C helped to interpret data analysis. S.K.W, H.G.L, H.M.O. and C.Y.A. revised the manuscript. All authors reviewed and approved the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1206_MOESM1_ESM.docx (484 kb)
Supplementary material 1 (DOCX 483 kb)


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Cell Factory Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea
  2. 2.University of Science and Technology (UST)DaejeonRepublic of Korea
  3. 3.Marine Microbiology, Department of Marine Ecosystem Dynamics, Atmosphere and Ocean Research InstituteUniversity of TokyoKashiwaJapan

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