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Haloprofundus halophilus sp. nov., isolated from the saline soil of Tarim Basin

  • Qin Xu
  • Heng-Lin Cui
  • Fanwei Meng
Original Paper

Abstract

A novel halophilic archaeon, designated NK23T, was isolated from an inland saline soil sampled from Xinjiang, China. The cells of strain NK23T were observed to be pleomorphic, to stain Gram-negative and form red-pigmented colonies on agar plates. The strain can grow at 25–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 2.1 M), at 0–1.0 M MgCl2 (optimum 0.05 M) and at pH 6.5–9.5 (optimum pH 7.0). The polar lipids were found to be phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, mannosyl glucosyl diether, sulfated mannosyl glucosyl diether, and three minor unidentified glycolipids, which were chromatographically identical to those detected in Haloprofundus (Hpf.) marisrubri CGMCC 1.14959T. On the basis of 16S rRNA gene and rpoB′ gene sequence similarities and phylogenetic analysis, strain NK23T was found to be related to Hpf. marisrubri CGMCC 1.14959T (97.8% and 94.1% similarities, respectively). The average nucleotide identity values and in silico DNA–DNA hybridization values between strain NK23T and Hpf. marisrubri SB9T were 85.22% and 29.3%, respectively. The DNA G+C content of the novel strain was determined to be 65.29 mol%. Based on the phenotypic and chemotaxonomic data, together with phylogenetic relationships, strain NK23T (= CGMCC 1.14944T = JCM 30670T) is considered to represent a new species of the genus Haloprofundus, for which the name Haloprofundus halophilus sp. nov. is proposed.

Keywords

Haloprofundus halophilus sp. nov. Halophilic archaeon Saline soil 

Notes

Acknowledgements

We are grateful to Prof. Yu-Guang Zhou (CGMCC) for kindly providing the reference type strain used in this study. This research work was financially supported by the National Natural Science Foundation of China (Nos. 4151101015, 31770005), the National Science & Technology Infrastructure Program of China (No. 2017FY100302) and Bureau of International Co-operation, Chinese Academy of Sciences.

Compliance with ethical standards

Conflicts of interest

The authors declares that they have no conflict of interest.

Ethical standards

The article does not contain any studies related to human participants or animals.

Supplementary material

10482_2018_1186_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1339 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.School of Food and Biological EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology and Center for Excellence in Life and PaleoenvironmentChinese Academy of SciencesNanjingPeople’s Republic of China

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