Haloprofundus halophilus sp. nov., isolated from the saline soil of Tarim Basin
A novel halophilic archaeon, designated NK23T, was isolated from an inland saline soil sampled from Xinjiang, China. The cells of strain NK23T were observed to be pleomorphic, to stain Gram-negative and form red-pigmented colonies on agar plates. The strain can grow at 25–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 2.1 M), at 0–1.0 M MgCl2 (optimum 0.05 M) and at pH 6.5–9.5 (optimum pH 7.0). The polar lipids were found to be phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, mannosyl glucosyl diether, sulfated mannosyl glucosyl diether, and three minor unidentified glycolipids, which were chromatographically identical to those detected in Haloprofundus (Hpf.) marisrubri CGMCC 1.14959T. On the basis of 16S rRNA gene and rpoB′ gene sequence similarities and phylogenetic analysis, strain NK23T was found to be related to Hpf. marisrubri CGMCC 1.14959T (97.8% and 94.1% similarities, respectively). The average nucleotide identity values and in silico DNA–DNA hybridization values between strain NK23T and Hpf. marisrubri SB9T were 85.22% and 29.3%, respectively. The DNA G+C content of the novel strain was determined to be 65.29 mol%. Based on the phenotypic and chemotaxonomic data, together with phylogenetic relationships, strain NK23T (= CGMCC 1.14944T = JCM 30670T) is considered to represent a new species of the genus Haloprofundus, for which the name Haloprofundus halophilus sp. nov. is proposed.
KeywordsHaloprofundus halophilus sp. nov. Halophilic archaeon Saline soil
We are grateful to Prof. Yu-Guang Zhou (CGMCC) for kindly providing the reference type strain used in this study. This research work was financially supported by the National Natural Science Foundation of China (Nos. 4151101015, 31770005), the National Science & Technology Infrastructure Program of China (No. 2017FY100302) and Bureau of International Co-operation, Chinese Academy of Sciences.
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Conflicts of interest
The authors declares that they have no conflict of interest.
The article does not contain any studies related to human participants or animals.
- Collins MD (1985) Isoprenoid quinone analysis in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–287Google Scholar
- Gupta RS, Naushad S, Baker S (2015) Phylogenomic analyses and molecular signatures for the class Halobacteria and its two major clades: a proposal for division of the class Halobacteria into an emended order Halobacteriales and two new orders, Haloferacales ord. nov. and Natrialbales ord. nov., containing the novel families Haloferacaceae fam. nov. and Natrialbaceae fam. nov. Int J Syst Evol Microbiol 65:1050–1069CrossRefGoogle Scholar
- Gupta RS, Naushad S, Fabros R, Adeolu M (2016) A phylogenomic reappraisal of family-level divisions within the class Halobacteria: proposal to divide the order Halobacteriales into the families Halobacteriaceae, Haloarculaceae fam. nov., and Halococcaceae fam. nov., and the order Haloferacales into the families, Haloferacaceae and Halorubraceae fam nov. Antonie Van Leeuwenhoek 109:565–587CrossRefGoogle Scholar
- Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, He G, Chen Y, Pan Q, Liu Y, Tang J, Wu G, Zhang H, Shi Y, Liu Y, Yu C, Wang B, Lu Y, Han C, Cheung DW, Yiu S-M, Peng S, Zhu X, Liu G, Liao X, Li Y, Yang H, Wang J, Lam TW, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. GigaScience 1:18CrossRefGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar