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Mucilaginibacter formosus sp. nov., a bacterium isolated from road-side soil

  • Md. Amdadul Huq
  • Shahina Akter
  • Sun-Young Lee
Original Paper

Abstract

A Gram-stain negative, aerobic, non-motile and rod-shaped novel bacterial strain, designated as MAH-5T, was isolated from a road-side soil sample and was characterised by using a polyphasic taxonomic approach. The colonies were observed to be yellowish orange in colour, smooth, circular and 0.3–0.7 mm in diameter when grown on nutrient agar for 2 days. Strain MAH-5T was found to be able to grow at 15–35 °C and at pH 4.0–8.0. The strain was observed to be positive for both the catalase and oxidase tests. Cells were found to be able to hydrolyse aesculin, gelatin and starch. By 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Mucilaginibacter and to be closely related to Mucilaginibacter panaciglaebae BXN5-31T (98.35%), Mucilaginibacter soyangensis HME6664T (97.82%), Mucilaginibacter antarcticus S14-88T (97.49%) and Mucilaginibacter ximonensis XM-003T (97.06%). In DNA–DNA hybridization tests, the DNA relatedness values between strain MAH-5T and its close phylogenetic neighbors were below 45.0%. The genomic DNA G + C content of strain MAH-5T was determined to be 41.5 mol% and the predominant isoprenoid quinine was identified as MK-7. The major fatty acids were identified as C15:0 iso and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c). The genetic characteristics, in combination with chemotaxonomic and physiological data, demonstrated that the isolated strain MAH-5T represents a novel species within the genus Mucilaginibacter, for which the name Mucilaginibacter formosus sp. nov. is proposed, with MAH-5T as the type strain (= KACC 19291T = CGMCC1.16489T).

Keywords

Mucilaginibacter formosus Gram-staining negative 16S rRNA gene Fatty acid 

Notes

Acknowledgements

This study was performed with the support of the Cooperative Research Program of the National Research Foundation of Korea grant (Project No. NRF-2016R1A2B4014591) funded by the Korea government (MISP), Republic of Korea.

Author’s contributions

MAH and SYL conceived the original screening and research plans. MAH supervised the experiments and wrote the article with contributions of all the authors. MAH and SA performed all of the experiments.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1183_MOESM1_ESM.pptx (99 kb)
Supplementary Fig. S1. The maximum-likelihood (ML) tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain MAH-5T and members of the genus Mucilaginibacter. Bootstrap values less than 50% are not shown (PPTX 99 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Department of Food and Nutrition, College of Biotechnology and Natural ResourceChung-Ang UniversityAnseong-SiRepublic of Korea
  2. 2.Department of Horticultural Life ScienceHankyong National UniversityAnseong-SiRepublic of Korea

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