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Antonie van Leeuwenhoek

, Volume 112, Issue 2, pp 319–327 | Cite as

Rhizobium album sp. nov., isolated from a propanil-contaminated soil

  • Ping Hang
  • Long Zhang
  • Xi-Yi Zhou
  • Qiang Hu
  • Jian-Dong JiangEmail author
Original Paper
  • 46 Downloads

Abstract

A novel Gram-stain negative, facultatively anaerobic, non-spore-forming, motile and rod-shaped bacterium (NS-104T) was isolated from a propanil-contaminated soil in Nanjing, China. Growth occurred at pH 5.0–9.0 (optimum 6.0), 16–37 °C (optimum 30 °C) and in the presence of 0–2.0% (w/v) NaCl (optimum, without NaCl). Strain NS-104T showed high 16S rRNA gene sequence identity to Rhizobium azooxidifex DSM 100211T (96.7%). The phylogenetic analysis of the 16S rRNA gene as well as the housekeeping genes recA, atpD and glnA demonstrated that strain NS-104T belongs to the genus Rhizobium. Strain NS-104T did not form nodules on six different legumes, and the nodD, nodC and nifH genes were neither amplified by PCR nor found in the draft genome of strain NS-104T. The sole respiratory quinone was ubiquinone Q-10. The polar lipid profile included the major amounts phosphatidylmonomethylethanolamine, phosphatidylglycerol and moderate amounts of phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and unidentified aminolipids. The major cellular fatty acids were C18:1ω7c (39.6%), C19:0 cyclo ω8c (29.8%) and C16:0 (11.5%). The G + C content of strain NS-104T was 61.9 mol%. Strain NS-104T therefore represents a new species, for which the name Rhizobium album sp. nov. is proposed, with the type strain NS-104T (= KCTC 62327T = CCTCC AB 2017250T).

Keywords

Rhizobium album sp. nov. Gram-stain negative 16S rRNA gene The housekeeping genes 

Notes

Acknowledgements

This work was financially supported by Grants from the National Natural Science Foundation of China (31670111) and the National Key Research and Development Program of China (2016YFD0800203).

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

10482_2018_1160_MOESM1_ESM.docx (861 kb)
Supplementary material 1 (DOCX 861 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Ping Hang
    • 1
  • Long Zhang
    • 1
  • Xi-Yi Zhou
    • 1
  • Qiang Hu
    • 1
  • Jian-Dong Jiang
    • 1
    Email author
  1. 1.Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China

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