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Antonie van Leeuwenhoek

, Volume 112, Issue 2, pp 159–165 | Cite as

Marinicaulis aureum sp. nov., isolated from a culture of the green alga Ulva prolifera, and emended description of the genus Marinicaulis

  • Zengmeng Wang
  • Zenghu ZhangEmail author
  • Chengcheng Li
  • Zhenzhen Hu
  • Hanshuang Zhao
  • Yongyu Zhang
Original Paper
  • 83 Downloads

Abstract

A Gram-stain negative, facultatively anaerobic, rod-shaped motile bacterium with a single polar flagellum, designed strain HHTR114T, was isolated from a culture of the green alga Ulva prolifera obtained from offshore seawater at Qingdao, China. Optimum growth occurred in the presence of 2–3% (w/v) NaCl, at pH 7.0–8.0 and 30 °C. The major fatty acids (> 10% of total fatty acids) were C16:0 (24.7%), C18:1ω7c 11-methyl (24.3%) and summed feature 3 (C16:1ω6c and/or C16:1ω7c, 19.7%). The major polar lipids were phosphatidylethanolamine, glycolipid and four unidentified polar lipids. The DNA G + C content of strain HHTR114T calculated on the basis of the genome sequence was 58.2% and the genome size is 4.1 Mbp. The predominant isoprenoid quinone was Q-10. The estimated DNA–DNA hybridization values were 21.4% [18.6–24.4%] between strain HHTR114T and Marinicaulis flavus SY-3-19T. On the basis of polyphasic analysis, strain HHTR114T is considered to represent a novel species, for which the name Marinicaulis aureum sp. nov. is proposed. The type strain of the type species is HHTR114T (= KCTC 62394T = MCCC 1K03481T).

Keywords

Polyphasic taxonomy Marinicaulis aureum sp. nov. 16S rRNA gene Ulva prolifera 

Notes

Acknowledgements

We are grateful to the Japan Collection of Microorganism and the Korean Collection for Type Cultures for providing the reference type strains.

Funding

This work was supported by the open task of Qingdao National Laboratory for Marine Science and Technology (QNLM2016ORP0311), the National Key Research and Development Program of China (2016YFA0601402), the CNOOC Tianjin project (CNOOC-KJ 125 FZDXM 00TJ 001-2014) and Natural Science Foundation of China (No. 31700104). This study is a contribution to the international IMBER project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

10482_2018_1139_MOESM1_ESM.docx (769 kb)
Supplementary material 1 (DOCX 769 kb)

References

  1. Auch AF, Klenk HP, Göker M (2010a) Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2:142–148CrossRefGoogle Scholar
  2. Auch AF, von Jan M, Klenk HP, Göker M (2010b) Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134CrossRefGoogle Scholar
  3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 3rd edn. Wiley, New YorkGoogle Scholar
  4. Beveridge TJ, Lawrence JR, Murray RG (2007) Sampling and staining for light microscopy. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al (eds) Methods for general and molecular microbiology. American Society of Microbiology, Washington, pp 19–33Google Scholar
  5. Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868CrossRefGoogle Scholar
  6. Cho JC, Giovannoni SJ (2003) Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53:1031–1036CrossRefGoogle Scholar
  7. Collins MD (1994) Isoprenoid quinones. In: Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 265–309Google Scholar
  8. Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715CrossRefGoogle Scholar
  9. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefGoogle Scholar
  10. Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207CrossRefGoogle Scholar
  11. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  12. Lyman J, Fleming RH (1940) Composition of sea water. J Mar Res 3:134–146Google Scholar
  13. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60CrossRefGoogle Scholar
  14. Minnikin DE, O’donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  15. Rosselló-Móra R, Trujillo ME, Sutcliffe IC (2017) Introducing a digital protologue: a timely move towards a database-driven systematics of archaea and bacteria. Antonie Van Leeuwenhoek 110:455–456CrossRefGoogle Scholar
  16. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. MIDI, NovelarkGoogle Scholar
  17. Shen P, Chen X-D (2008) Experiment of microbiology. Higher Education Press (English translation), BeijingGoogle Scholar
  18. Sun C, Wu C, Su Y, Wang R-J, Fu G-Y, Zhao Z, Yu X-Y, Huang M-M, Han B-N, Lv Z-B, Wu M (2018) Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater. Int J Syst Evol Microbiol 67:4024–4031CrossRefGoogle Scholar
  19. Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM (eds) Methods for general and molecular microbiology. American Society of Microbiology, Washington, pp 330–393Google Scholar
  20. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y, Seo H, Chun J (2016) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617Google Scholar
  21. Yu X-Y, Yu X-D, Fu G-Y, Zhao Z, Shen X, Sun C, Wu M (2018) Marinicaulis flavus gen. nov., sp. nov., a novel stalked bacterium of the family Parvularculaceae. Int J Syst Evol Microbiol 68:2061–2067CrossRefGoogle Scholar
  22. Zhang Z-L, Zhang X-Q, Wu N, Zhang W-W, Zhu X-F, Cao Y, Wu M (2014) Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 64:2805–2811CrossRefGoogle Scholar
  23. Zhong Z, Liu Y, Wang F, Zhou Y, Liu H, Liu Z (2016) Aquisalinus flavus gen. nov., sp. nov., a member of the family Parvularculaceae isolated from a saline lake. Int J Syst Evol Microbiol 66:1813–1817CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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