Antonie van Leeuwenhoek

, Volume 112, Issue 1, pp 67–74 | Cite as

Draft genome sequence of the symbiotic Frankia sp. strain BMG5.30 isolated from root nodules of Coriaria myrtifolia in Tunisia

  • Abdellatif Gueddou
  • Erik Swanson
  • Karima Hezbri
  • Imen Nouioui
  • Amir Ktari
  • Stephen Simpson
  • Krystalynne Morris
  • W. Kelley Thomas
  • Faten Ghodhbane-Gtari
  • Maher GtariEmail author
  • Louis S. TisaEmail author
Original Paper


Frankia sp. strain BMG5.30 was isolated from root nodules of a Coriaria myrtifolia seedling on soil collected in Tunisia and represents the second cluster 2 isolate. Frankia sp. strain BMG5.30 was able to re-infect C. myrtifolia generating root nodules. Here, we report its 5.8-Mbp draft genome sequence with a G + C content of 70.03% and 4509 candidate protein-encoding genes.


Actinobacteria Actinorhizal symbiosis Hydrogenase Nitrogen fixation Natural products Host microbe interactions Genomes 



Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2740. This work was also supported by the USDA National Institute of Food and Agriculture Hatch 022821 (LST), Agriculture and Food Research Initiative Grant 2015-67014-22849 from the USDA National Institute of Food and Agriculture (LST), and the College of Life Science and Agriculture at the University of New Hampshire-Durham. Sequencing was performed on an Illumina HiSeq 2500 purchased with an NSF MRI Grant: DBI-1229361 to WK Thomas.

Authors Contribution

MG, FGG, and LST conceived the study. AG, ES, HK. AK, IN, KM, ST, and WKT performed the research. ES, AG, MG, FGG, and LST analaized the data. AG, ES, MG, FGG, and LST wrote the manuscript. All the authors approved the paper.

Compliance with ethical standards

Conflict of interest

The authors have declared that they have no competing interest exists.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2018_1138_MOESM1_ESM.pdf (51 kb)
Supplementary material 1 (PDF 51 kb)
10482_2018_1138_MOESM2_ESM.xlsx (76 kb)
ESM1: COG analysis for the Frankia cluster 2 genomes (XLSX 76 kb)
10482_2018_1138_MOESM3_ESM.xlsx (174 kb)
ESM2: BMG5.30 and BMG5.1 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 173 kb)
10482_2018_1138_MOESM4_ESM.xlsx (32 kb)
ESM3: Core genome from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 31 kb)
10482_2018_1138_MOESM5_ESM.xlsx (110 kb)
ESM4: Singleton genes for the cluster 2 genomes (XLSX 110 kb)
10482_2018_1138_MOESM6_ESM.xlsx (40 kb)
ESM5: BMG5.30, BMG5.1 and Dg1 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 39 kb)
10482_2018_1138_MOESM7_ESM.xlsx (34 kb)
ESM6: BMG5.30, BMG5.1 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 33 kb)
10482_2018_1138_MOESM8_ESM.xlsx (20 kb)
ESM7: BMG5.30and Dg1 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 20 kb)
10482_2018_1138_MOESM9_ESM.xlsx (51 kb)
ESM8: BMG5.30 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 51 kb)
10482_2018_1138_MOESM10_ESM.xlsx (22 kb)
ESM9: BMG5.30, Dg1 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 21 kb)
10482_2018_1138_MOESM11_ESM.xlsx (17 kb)
ESM10: BMG5.1 and Dg1 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 16 kb)
10482_2018_1138_MOESM12_ESM.xlsx (24 kb)
ESM11: BMG5.1 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 24 kb)
10482_2018_1138_MOESM13_ESM.xlsx (37 kb)
ESM12: BMG5.1, Dg1 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (XLSX 36 kb)
10482_2018_1138_MOESM14_ESM.pdf (39 kb)
ESM13: Dg1 and Dg2 overlap genes from Venn diagram (Figure 3) among the cluster 2 genomes (PDF 39 kb)


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Institut National des Sciences Appliquées et de TechnologieUniversité CarthageTunisTunisia
  2. 2.Laboratoire Microorganismes et Biomolécules ActivesUniversité Tunis El Manar (FST)TunisTunisia
  3. 3.Department of Molecular, Cellular, and Biomedical SciencesUniversity of New HampshireDurhamUSA

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