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Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater

  • Min-Ling Zheng
  • Jian-Yu Jiao
  • Lei Dong
  • Ming-Xian Han
  • Liang-Hui Li
  • Min Xiao
  • Cha Chen
  • Ping-Hua QuEmail author
  • Wen-Jun LiEmail author
Original Paper
  • 57 Downloads

Abstract

A Gram-negative, aerobic, non-motile and non-spore forming bacterium, designated strain SYSU WZ-2T, was isolated from an estuarine seawater sample. Growth of strain SYSU WZ-2T was observed at temperature range of 10–40° C (optimum, 32 °C), pH range of 6–10 (optimum, pH 7–8) and in the presence of up to 5.0% NaCl (w/v). The DNA G+C content of the novel strain was determined to be 30.1% (genome). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified phospholipids. The major fatty acids were C18:0 3-OH (27.5%), C18:1ω9c (19.3%), C16:0 (17.0%) and C14:0 (12.9%). The respiratory quinone was found to be ubiquinone Q8. Pairwise comparison of the 16S rRNA gene sequence showed that strain SYSU WZ-2T shares high identities with members of the genera Francisella (94.8–95.9%) and Allofrancisella (93.8–94.2%). The phylogenetic dendrograms based on 16S rRNA gene sequences with the members of the family Francisellaceae showed that the strain SYSU WZ-2T formed a distinct phylogenetic lineage well separated from the members of the genera Francisella and Allofrancisella. MALDI-TOF mass spectrometric analysis also depicted a different profile for strain SYSU WZ-2T compared with those of members of the genera Francisella and Allofrancisella. Based on the above results and differences in phenotypic and chemotaxonomic features, strain SYSU WZ-2T is characterized to represent a new species of a novel genus, for which the name Pseudofrancisella aestuarii gen. nov., sp. nov. is proposed (type strain SYSU WZ-2T = KCTC 52557T = CGMCC 1.13718T).

Keywords

Pseudofrancisella aestuarii gen. nov., sp. nov. Estuarine seawater Polyphasic taxonomy 

Notes

Acknowledgements

Ping-Hua Qu was supported by Research Fund for Outstanding Young in Higher Education Institutions of Guangdong Province (2013 LYM_0015), and Science and Technology Planning Project of Guangdong Province, China (2017A0202150683). Wen-Jun Li was supported by the Guangdong Province Higher Vocational Colleges and Schools Pearl Scholar Founded Scheme (2014).

Author contributions

WJL and PHQ designed research and project outline. MLZ, LD, MXH, LHL and MX performed isolation, deposition and identification. PHQ and CC performed MALDI-TOF–MS analysis. JYJ and WJL performed genome analysis. PHQ, MLZ and WJL drafted the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

All the authors have declared no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2018_1220_MOESM1_ESM.doc (2.3 mb)
Supplementary material 1 (DOC 2335 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.The Second Clinic CollegeGuangzhou University of Chinese MedicineGuangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  3. 3.Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouPeople’s Republic of China
  4. 4.College of FisheriesHenan Normal UniversityXinxiangPeople’s Republic of China

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