Pseudonocardia tritici sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.)
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An aerobic, non-motile, Gram-stain positive actinomycete, designated strain NEAU-YY211T, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, mid-eastern China, and characterised taxonomically using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequence indicated that strain NEAU-YY211T belongs to the genus Pseudonocardia, showing high similarities with respect to Pseudonocardia ammonioxydans H9T (99.1%) and Pseudonocardia antitumoralis SCSIO 01299T (99.0%), respectively. The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as arabinose and galactose. The predominant menaquinone of strain NEAU-YY211T was identified as MK-8(H4) and the major fatty acids were identified as iso-C16:0, C17:1ω8c and iso-C16:1. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The G+C content of the genomic DNA was determined to be 72.6 mol%. Levels of DNA–DNA relatedness with P. ammonioxydans JCM 12462T and P. antitumoralis DSM 45322T were 54.5 ± 3.5% and 49.8 ± 2.5% (mean ± SD), respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate can be distinguished from closely related type strains and thus represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia tritici sp. nov. is proposed. The type strain is NEAU-YY211T (= DSM 106068T = CGMCC 4.7474T).
KeywordsPseudonocardia tritici sp. nov. Polyphasic analysis 16S rRNA gene Phenotypic characteristics
This work was supported in part by grants from the National Natural Science Foundation of China (No. 31700067), the Natural Science Foundation of Heilongjiang Province (LC2016011), the ‘Young Talents’ Project of Northeast Agricultural University (14QC04), Key Laboratory of Agricultural Microbiology of Heilongjiang Province (NW2017001), the China Postdoctoral Science Foundation (2015M580255) and the Heilongjiang Provincial Postdoctoral Science Foundation (LBH-Z15016). We are grateful to Professor Aharon Oren for helpful advice on the specific epithet.
JS performed the laboratory experiments, analysed the data, and drafted the manuscript. SQ contributed to the biochemical characterisation. JZ and YW contributed to the polyphasic taxonomy. CH contributed to the morphological analyses. SJ and XS contributed to the fatty acids determination. XW and WX designed the experiments and revised the manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.
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