Advertisement

Agroforestry Systems

, Volume 93, Issue 4, pp 1285–1295 | Cite as

Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

  • Afshan Naaz
  • Sheikh Altaf Hussain
  • Ruphi Naz
  • Mohammad AnisEmail author
  • Abdulrahman A. Alatar
Article

Abstract

Syzygium cumini L. (Myrtaceae) is an evergreen medicinal tree confined to tropical and subtropical regions of the world and is mainly recognized for healing of type, two diabetes. An efficient indirect regeneration system has been standardized for this tree using cotyledonary node, inter-node, nodal and leaf segment explants excised from 10 days old aseptic seedling. For callus induction explants were incubated on Murashige and Skoog (1962) medium supplemented with different concentrations of 2, 4-Dichlorophenoxy acetic acid (2, 4-D) or 2, 4, 5-Trichloro phenoxy acetic acid (2, 4, 5-T). All the tested concentrations facilitated callus initiation with different types and varying regeneration potentialities. The regenerated callus on different treatments showed variation in color and texture and best callogenesis (75%) was observed on (7.5 µM) 2, 4-D after 4 weeks. Different morphogenic response was observed when callus was transferred to a secondary organogenic medium fortified with (5.0 µM) benzyl adenine or Kinetin. The yellow white friable (YWF) callus obtained from CN explants exhibited appearance of small leafy structures on secondary medium. Not all types of calli originating from various types of explant were organogenic. Best shoot induction (93%) were observed in (YWF) type of callus obtained from CN explants on MS medium enriched with 5.0 µM BA + 0.25 µM NAA + 10 mg/l AgNO3 with maximum mean shoot number (10.19 ± 0.05) and mean length (4.12 ± 0.32 cm) per clump after 4 weeks of transfer. However the shoot number got increased up to 48 shoots/clump on subsequent subculturing after 12 weeks. For ex vitro rooting shoot lets (4 cm) were excised and pulse treated in full or half strength MS medium augmented with varied concentrations of IBA. Best rhizogenesis (80%) was achieved on 200 µM IBA treated shootlets after 4 weeks of transfer to sterilized soilrite. The regenerants were successfully acclimatized and established in earthen pots with 90% survival.

Keywords

De-differentiation Recalcitrant Phenolic exudation Ex vitro rooting Histology AgNO3 

Abbreviations

BA

Benzyl adenine

Kin

Kinetin

IBA

Indole-3-butyric acid

MS

Murashige and Skoogs medium

Min

Minute

2, 4-D

2, 4-Dichlorophenoxy acetic acid

2, 4, 5-T

2, 4, 5-Trichlorophenoxy acetic acid

Notes

Acknowledgements

The authors extend their appreciation to the International Scientific Partnership Program (ISPP) at King Saud University for funding this research work through ISPP #0082. Award of UGC—BSR faculty fellowship (2017) to MA is duly acknowledged.

Author’s contribution

AN, SAH and RN performed the experiment, collected and analyzed data, wrote the manuscript. MA conceived and designed the experiments and edited the manuscript. AAA revised the manuscript and gave valuable suggestions.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

References

  1. Abbas RM, Khan M, Fatima FF (2003) Studies on Jaman (Syzygium cuminii L. Skeels) seed storage behavior. Pak J Agric Sci 40:3–4Google Scholar
  2. Abdelwahd R, Hakam N, Labhilili M, Udape SM (2006) Use of adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of Faba bean. Afr J Biotechnol 7:997–1002Google Scholar
  3. Ahmad N, Javed SB, Khan MI, Anis M (2013) Rapid plant regeneration and analysis of genetic fidelity in micropropagated plants of Vitex trifolia: an important medicinal plant. Acta Physiol Plant 35:2493–2500CrossRefGoogle Scholar
  4. Alikatte KL, Akondi BR, Yerragunta VG, Veerareddy PR, Palle S (2012) Antiamnesic activity of Syzygium cumini against scopolamine induced spatial memory impairments in rats. Brain Dev. 10:844–851CrossRefGoogle Scholar
  5. Anis M, Ahmad N (2016) Plant tissue culture: propagation conservation and crop improvement. Springer, SingaporeCrossRefGoogle Scholar
  6. Anonymous (2001) The wealth of India, raw material, Vol-R Publication and information directorate CSIR, New Delhi, p 63–65Google Scholar
  7. Ayyanar M, Babu PS (2012) Syzygium cumini (L.) Skeels: a review of its phytochemical constituents and traditional uses. Asian Pac J Trop Biomed 3:40–246Google Scholar
  8. Bandyopadhyay S, Cane K, Rasmussen G, Hamill JD (1999) Efficient plant regeneration from seedling explants of two commercially important temperate eucalypts species, Eucalyptus nitens and E. globilus. Plant Sci 140:189–198CrossRefGoogle Scholar
  9. Cai Z, Jing X, Tian X, Jiang J, Liu F, Wang X (2015) Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus eurphartica Olv. S Afr J Bot 97:143–148CrossRefGoogle Scholar
  10. Chiruvella KK, Mohammed A, Ghanta RG (2014) Factors influencing the seed germination of soymidafe brifuga (roxb.) A. juss (Meliaceae). Trakia J Sci 2:121–131Google Scholar
  11. Cvjetko P, Milosic A, Domijan AM, Vrcec IV, Tolic S, Stefanic PP, Letofsky I, Letofsky P, Tkalec M, Balen B (2017) Toxicity of silver ions and differently coated silver nanoparticles in Allium cepa roots. Ecotoxicol Environ Saf 137:18–28CrossRefGoogle Scholar
  12. Fatima N, Anis M (2012) Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal. Physiol Mol Biol Plants 18:59–67CrossRefGoogle Scholar
  13. George EF, Hall MA, De Klerk G-J (2008) Plant propagation by tissue culture, 3rd edn. Springer, NetherlandGoogle Scholar
  14. Jain N, Babbar SB (2000) Recurrent production of plants of black plum, Syzygium cumini (L.) Skeels, a myrtaceous fruit tree from in vitro cultured seedling explants. Plant Cell Rep 19:519–524CrossRefGoogle Scholar
  15. Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York, pp 126–154Google Scholar
  16. Kumar MS, Nandi SC (2015) High frequency plant regeneration with histological analysis of organogenic callus from internode explants of Asteracantha longifolia Nees. J Genet Eng Biotechnol 13:31–37CrossRefGoogle Scholar
  17. Kumar A, Ilavarasan R, Jayachandran T, Deecaraman M, Kumar MR (2008) Anti-inflammatory activity of Syzygium cumini seed. Afr J Biotechnol 7:941–943Google Scholar
  18. Kumar V, Parvatam G, Ravishankar GA (2009) AgNO3-a potential regulator of ethylene activity and plant growth modulator. Electron J Biotechnol.  https://doi.org/10.2225/vol12-issue2-fulltext-1 Google Scholar
  19. Kumari KG, Ganesan M, Jayabalan N (2008) Somatic organogenesis and plant regeneration in Ricinus cummunis. Biol Plant 1:17–25CrossRefGoogle Scholar
  20. Lin C, Pei FU, Wu LS, Shen PF (2005) Micropropagation of Callistemon viminalis. J Fuji Fore Sci Technol 32:52–54Google Scholar
  21. Lloyd G, McCown B (1981) Commercially feasible micropropgation of mountain laurel, Kalmea latifolia, by the use of shoot tip culture. Int Plant Propag Soc 30:421–427Google Scholar
  22. Loomis WD, Battaile J (1966) Plant phenolic compounds and the isolation of plant enzymes. Phytochemistry 5:423–438CrossRefGoogle Scholar
  23. Mathew MK, Francis MS, Hariharan M (1987) Development of callus in cloves (Syzygium aromaticum (L) Merr and Perry). J Plant Crops 15:123–125Google Scholar
  24. Mohamad AA, Ali SI, El-Baz FK (2013) Antioxidant and antibacterial activities of crude extracts and essential oils of Syzygium cumini leaves. PLoS ONE 8(4):e60269CrossRefGoogle Scholar
  25. Murashige M, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  26. Naaz A, Shahzad A, Anis M (2014) Effect of adenine sulphate interaction on growth and development of shoot regeneration and inhibition of shoot tip necrosis under in vitro condition in adult Syzygium cumini L.—a multipurpose tree. Appl Biochem Biotechnol 173:90–102CrossRefGoogle Scholar
  27. Niemietz CM, Tyerman SD (2002) New potent inhibitors of aquaporins: silver and gold compounds inhibit aquaporins of plant and human origin. FEBS Lett 531:443–447CrossRefGoogle Scholar
  28. Perveen S, Anis M (2015) Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree. Agrofor Syst 89:721–733CrossRefGoogle Scholar
  29. Pospisilova J, Ticha I, Kadleaeek P, Haisel D, Plazakova S (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Planta 42:481–497CrossRefGoogle Scholar
  30. Poynton HC, Lazorchak JM, Impellitteri CA, Blalock BJ, Rogers K, Allen HJ, Loguinov A, Govindasmawy S (2012) Toxicogenomic responses of nanotoxicity in Daphnia magna exposed to silver nitrate and coated silver nanoparticles. Environ Sci Technol 46:6288–6296CrossRefGoogle Scholar
  31. Prasantha KG, Sathyanarayana BN, Mathew D, Sondur SN (2003) In vitro callus induction and plantlet regeneration in Rose apple (Syzygium Jambos L.). J Plant Biol 30:99–102Google Scholar
  32. Raj RSDP, Morais SM, Gopalakrishnan K (2010) In vitro propagation of Callistemon citrinus L. Ind J Sci Technol 3:0974–6846Google Scholar
  33. Rathore V, Shekhawat NS, Singh RP, Rathore JS, Daglen HR (2004) Cloning of adult trees of Jamun (Syzigium cumini). Ind J Biotechnol 3:241–254Google Scholar
  34. Reynolds JF, Murashige TA (1979) Sexual embryogenesis in callus culture of palms. In Vitro Cell Dev Biol 15:383–387CrossRefGoogle Scholar
  35. Sarropoulou V, Dimassi-Theriou K, Therios I (2016) Effect of the ethylene inhibitors silver nitrate, silver sulfate, and cobalt chloride on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela. Turk J Biol 40:670–683CrossRefGoogle Scholar
  36. Singh B, Virk GS, Nagpal AK (2011) An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush. Physiol Mol Biol Plants 17:161–169CrossRefGoogle Scholar
  37. Sujatha R, Babu LC, Nazeem P (2003) Histology and organogenesis from callus cultures of black pepper (Piper nigrum L.). J Tropic Agric 41:16–19Google Scholar
  38. Yadav U, Lal M, Jaiswal VS (1990) In vitro micropropagation of tropical fruit tree Syzigium cuminii L. Plant Cell Tiss Org Cult 21:87–92CrossRefGoogle Scholar
  39. Yang G, Lu Z (2007) In vitro callus initiation of guava. Acta Hort (ISHS) 738:501–506CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia

Personalised recommendations