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Angiogenesis

pp 1–11 | Cite as

A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis

  • Yunwei Shi
  • Xuchu Duan
  • Guangmin Xu
  • Xiaoning Wang
  • Guanyun Wei
  • Shikui Dong
  • Gangcai XieEmail author
  • Dong LiuEmail author
Brief Communication
  • 102 Downloads

Abstract

MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18–25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.

Keywords

MiRNA Ribosomal DNA Endothelial cells Zebrafish Angiogenesis 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China 81570447, 81870359; Natural Science Foundation from Jiangsu Province 17KJA180008, SWYY-048, and BK20180048.

Supplementary material

10456_2019_9663_MOESM1_ESM.pdf (103 kb)
Supplementary material 1 (PDF 102 KB)
10456_2019_9663_MOESM2_ESM.pdf (3.4 mb)
Supplementary material 2 (PDF 3462 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Co-innovation Center of Neuroregeneration, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationNantong UniversityNantongChina
  2. 2.School of Life ScienceNantong UniversityNantongChina
  3. 3.Department of Sports MedicineShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  4. 4.Medical School, Institute of Reproductive MedicineNantong UniversityNantongChina

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