A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis
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MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18–25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.
KeywordsMiRNA Ribosomal DNA Endothelial cells Zebrafish Angiogenesis
This work was supported by the National Natural Science Foundation of China 81570447, 81870359; Natural Science Foundation from Jiangsu Province 17KJA180008, SWYY-048, and BK20180048.
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