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Pollen dispersion in Myrica esculenta (Myricaceae): a dioecious anemophilous tree species of Himalaya

  • Vinod Prasad KhanduriEmail author
  • Arun Sukumaran
Original Paper
  • 5 Downloads

Abstract

Myrica esculenta is an important dioecious medicinal tree species of the Himalayan region. The role of floral morphology and pollen flow between the sexes in M. esculenta is unknown. Therefore, a study on floral morphology, pollen production, germination and pollen dispersal in M. esculenta was undertaken in a Himalayan temperate forest during 2015–2016 for one reproductive season. Pollen production and dispersion were observed in two habitat conditions, viz. tree outside forest (TOF) and natural forest (NF). Pollen flow was observed by exposing jelly-coated slides at geometrically increasing distances under two habitat conditions, i.e. TOF and NF. The pollen production per tree was significantly higher in TOF conditions (two times more than the NF). The total pollen production in the trees of outside forest was 3.10 × 1011 ± 0.58 × 1011 pollen per tree which was 1.60 × 1011 ± 0.42 × 1011 pollen per tree in natural forest condition. The fruit set was 6% more in TOF condition than the NF, indicating pollen limitation fecundity in M. esculenta. Pollen dispersal strongly differed between TOF and NF.

Keywords

Pollination Pollen viability Pollen germination Pollen migration Myrica Himalaya 

Notes

Acknowledgements

The authors are thankful to all the three anonymous reviewers of the earlier draft of this manuscript for their valuable suggestions to improve the MS.

Authors’ contribution

VPK and AS conceived the study, set up the experiment, conducted fieldwork and drafted the initial manuscript; VPK revised the MS thoroughly. Both authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Ashman, T. L., & Diefenderfer, C. (2001). Sex ratio represents a unique context for selection on attractive traits: Consequences for the evolution of sexual dimorphism. American Naturalist, 157, 334–347.CrossRefGoogle Scholar
  2. Bhatt, I. D., Rawal, R. S., & Dhar, U. (2000). The availability, fruit yield, and harvest of Myrica esculenta in Kumaun (West Himalaya), India. Mountain Research and Development, 20, 146–153.CrossRefGoogle Scholar
  3. Bittencourt, J. V. M., & Sebbenn, A. M. (2008). Pollen movement within a continuous forest of wind-pollinated Araucaria angustifolia, inferred from paternity and TwoGener analysis. Conservation Genetics, 9(4), 855–868.CrossRefGoogle Scholar
  4. Broadhurst, L. (2015). Pollen dispersal in fragmented populations of the dioecious wind-pollinated tree, Allocasuarina verticillata (Drooping Sheoak, Drooping She-Oak; Allocasuarinaceae). PLoS ONE, 10(3), e0119498.  https://doi.org/10.1371/journal.pone.0119498.CrossRefGoogle Scholar
  5. de Jong, T. J., Batenburg, J. C., & Klinkhamer, P. G. L. (2005). Distance-dependent pollen limitation of seed set in some insect-pollinated dioecious plants. Acta Oecologica, 28, 331–335.CrossRefGoogle Scholar
  6. Gaur, R. D. (1999). Flora of the District Garhwal: North West Himalaya (with Ethnobotanical Notes). Srinagar (Garhwal): Transmedia.Google Scholar
  7. Gusain, Y. K., & Khanduri, V. P. (2016). Myrica esculenta wild edible fruit of Indian Himalaya: Need a sustainable approach for indigenous utilization. Ecology Environment & Conservation, 22(April Suppl.), S267–S267.Google Scholar
  8. Jeeva, S., Lyndem, F. G., Sawian, J. T., Laloo, R. C., & Mishra, B. P. (2011). Myrica esculenta Buch.-Ham. ex D. Don.-A potential ethnomedicinal species in a subtropical forest of Meghalaya, northeast India. Asian Pacific Journal of Tropical Biomedicine, S, 174–177.CrossRefGoogle Scholar
  9. Khanduri, V. P. (2012). Temporal and spatial variation of pollen yield in natural populations of Pinus roxburghii. Forestry Studies in Chiana, 14(1), 20–29.CrossRefGoogle Scholar
  10. Khanduri, V. P., Kumar, K. S., & Sharma, C. M. (2015). Role of pollen production in mating success in some tropical tree species. Brazilian Journal of Botany, 38, 107–112.CrossRefGoogle Scholar
  11. Khanduri, V. P., Kumar, K. S., Sharma, C. M., Riyal, M. K., & Kar, K. (2019a). Pollen limitation and seed set associated with year-to-year variation in flowering of Gmelina arborea in a natural tropical forest. Grana, 58(2), 133–143.CrossRefGoogle Scholar
  12. Khanduri, V. P., & Sharma, C. M. (2002). Pollen productivity variations, microsporangium dehiscence and pollen flow in Himalayan Cedar (Cedrus deodara Roxb. ex D. Don). Annals of Botany, 89, 587–593.CrossRefGoogle Scholar
  13. Khanduri, V. P., Sukumaran, A., & Sharma, C. M. (2019b). Male-skewed sex ratio in Myrica esculenta: A dioecious tree species. Trees.  https://doi.org/10.1007/s00468-019-01850-5.Google Scholar
  14. Ksanbok, M., Lynser, M. B., & Pala, K. H. M. (2014). Marketing of indigenous fruits: A source of income among Khasi women of Meghalaya, North East India. Journal of Agriculture Science, 5, 1–9.Google Scholar
  15. Kumar, K. S., Khanduri, V. P., Kar, K., Sharma, C. M., & Riyal, M. K. (2016). Effect of growth regulators and time on in vitro pollen germination in three ornamental tropical tree species. Journal of Agricultural Sciences and Technology, 18, 1247–1255.Google Scholar
  16. Kumar, A., & Rana, A. C. (2013). Pharmacognostic and pharmacological profile of traditional medicinal plant: Myrica nagi. International Research Journal of Pharmacology, 3, 32–37.Google Scholar
  17. Kumari, P., Joshi, G. C., & Tewari, L. M. (2011). Diversity and status of ethnomedicinal trees of Almora district in Uttarakhand, India. International Journal of Biodiversity Conservation, 3, 298–326.Google Scholar
  18. Lowry, R. (1998). VassarStats: Website for statistical computation. Statistics in Medicine, 17(8), 857–872.CrossRefGoogle Scholar
  19. Marchelli, P., Smouse, P. E., & Gallo, L. A. (2012). Short-distance pollen dispersal for an outcrossed, wind-pollinated southern beech (Nothofagus nervosa (Phil.) Dim. et Mil.). Tree Genetics & Genomes, 8(5), 1123–1134.CrossRefGoogle Scholar
  20. Nainwal, P., & Kalra, K. (2009). Study on the wound activity potential on the aqueous extract of the bark of Myrica esculenta Buch. & Ham. International Journal of Pharmacology and Clinical Research, 1, 85–87.Google Scholar
  21. Öster, M., & Eriksson, O. (2007). Sex ratio mediated pollen limitation in the dioecious herb Antennaria dioica. Écoscience, 14, 387–398.CrossRefGoogle Scholar
  22. Otero-Arnaiz, A., & Oyama, K. (2001). Reproductive phenology, seed-set and pollination in Chamaedorea alternans, an understorey dioecious palm in a rain forest in Mexico. Journal of Tropical Ecology, 17, 745–754.CrossRefGoogle Scholar
  23. Raina, R., Behera, M. C., Chand, R., & Sharma, Y. (2003). Reproductive biology of Gentiana kurroo Royle. Current Science, 85, 667–670.Google Scholar
  24. Shah, S., Tewari, A., Tewari, B., & Singh, R. P. (2010). Seed maturity indicators in Myrica esculenta, Buch-Ham. Ex. D.Don.: A multipurpose tree species of subtropical temperate Himalayan region. New Forests, 40, 9–18.CrossRefGoogle Scholar
  25. Sharma, C. M., & Khanduri, V. P. (2007). Pollen-mediated gene flow in Himalayan long needle pine (Pinus roxburghii Sargent). Aerobiologia, 23, 153–158.CrossRefGoogle Scholar
  26. Sood, P., & Shri, R. (2018). A review on ethnomedicinal, phytochemical and pharmacological aspects of Myrica esculenta. Indian Journal of Pharmaceutical Sciences, 80(1), 2–13.Google Scholar
  27. Tuinstra, M. R., & Wedel, J. (2000). Estimation of pollen viability in grain sorghum. Crop Science, 40, 968–970.CrossRefGoogle Scholar
  28. Vamosi, J. C., Otto, S. P., & Barrett, S. C. H. (2003). Phylogenetic analysis of the ecological correlates of dioecy in angiosperms. Journal of Evolutionary Biology, 16, 1006–1018.CrossRefGoogle Scholar
  29. Wright, J. W. (1952). Pollen dispersion of some forest trees. Northeast Forest Experiment Station, Station Paper, 46, 42.Google Scholar
  30. Xia, J., Lu, J., Wang, Z. X., Hao, B. B., Wang, H. B., & Liu, G. H. (2013). Pollen limitation and Allee effect related to population size and sex ratio in the endangered Ottelia acuminata (Hydrocharitaceae): Implications for conservation and reintroduction. Plant Biology, 15, 376–383.CrossRefGoogle Scholar
  31. Yanthan, M., & Misra, A. K. (2013). Molecular approach to the classification of medicinally important actinorhizal genus Myrica. Indian Journal of Biotechnology, 12, 133–136.Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of ForestryVCSG Uttarakhand University of Horticulture and ForestryRanichauri, Tehri GarhwalIndia

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