nCup a 1 as a marker of allergy to cypress pollen
- 29 Downloads
The increase in polysensitisations among allergic patients has led us to search for suitable means of diagnosis for identifying true sensitisation, and distinguishing true sensitisation from cross-reactivity. Cross-reactive carbohydrate determinants (CCDs) present in glycoproteins from cypress pollen extracts have been linked with such cross-reactivity, particularly in in vitro assays. The application of component-resolved diagnosis using recombinant allergens makes it possible to identify true allergens. The problem arises when the allergen available for the usual diagnostic methods, which are used as a reference for the diagnosis of allergy to cypress pollen nCup a 1, is a native allergen. The aim of the study was to validate the native allergen nCup a as a marker of true sensitisation to cypress pollen. The sera of 96 subjects with a proven allergy to cypress pollen were analysed. We then quantified IgE specific to Cupressus arizonica and to nCup a 1 and also analysed the CCDs in subjects sensitised to several tree pollen allergens, presenting with MUXF3-specific IgE. Results revealed that there is a statistically significant correlation between conventional diagnostic techniques used to determine allergy to cypress pollen (SPT and IgE Cupressus arizonica) and sensitisation to nCup a 1. CCD quantification in subjects sensitised to several tree pollen antigens showed that these did not interfere with our results. We validated the native Cupressus arizonica allergen, nCup a 1, as a marker of allergy to cypress pollen in our population.
KeywordsCypress pollen allergy Cupressus arizonica Pectate lyase Component-resolved diagnosis Cup a 1
Cross-reactive carbohydrate determinants
Skin prick test
The authors wish to thank Fernando De la Torre from ALK laboratory for his collaboration.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Alisi, C., Afferni, C., Iacovacci, P., Barletta, B., Tinghino, R., Butteroni, C., et al. (2001). Rapid isolation, characterization, andglycan analysis of cup a 1, the major allergen of Arizona cypress(Cupressus arizonica) pollen. Allergy, 56(10), 978–984.Google Scholar
- Domínguez-Ortega, J., López-Matas, M. Á., Alonso, M. D., Feliú, A., Ruiz-Hornillos, J., González, E., et al. (2016). Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area. Allergy and Rhinology (Providence), 7(4), 200–206. https://doi.org/10.2500/ar.2016.7.0183.CrossRefGoogle Scholar
- Fernández-González, D., González-Parrado, Z., Vega-Maray, A. M., Valencia-Barrera, R. M., Camazón-Izquierdo, B., De Nuntiis, P., et al. (2010). Platanus pollen allergen, Pla a 1: Quantification in the atmosphere and influence on a sensitizing population. Clinical and Experimental Allergy, 40(11), 1701–1708. https://doi.org/10.1111/j.1365-2222.2010.03595.x.CrossRefGoogle Scholar
- Hidalgo, P. J., Galan, C., & Dominguez, E. (2003). Male phenology of three species of Cupressus: Correlation with airborne pollen. Trees, 17, 336–344.Google Scholar
- Iacovacci, P., Afferni, C., Butteroni, C., Pironi, L., Puggioni, E. M., Orlandi, A., et al. (2002). Comparison between the native glycosylated and the recombinant Cup a1 allergen: role of carbohydrates in the histamine release from basophils. Clinical and Experimental Allergy, 32(11), 1620–1627.CrossRefGoogle Scholar
- Perez-Badia, R., Rapp, A., Vaquero, C., & Fernandez-Gonzalez, F. (2011). Aerobiological study in east-central Iberian Peninsula: Pollen diversity and dynamics for major taxa. Annals of Agricultural and Environmental Medicine, 18(1), 99–111.Google Scholar
- Scala, E., Alessandri, C., Bernardi, M. L., Ferrara, R., Palazzo, P., Pomponi, D., et al. (2010). Cross-sectional survey on immunoglobulin E reactivity in 23 077 subjects using an allergenic molecule-based microarray detection system. Clinical and Experimental Allergy, 40(6), 911–921. https://doi.org/10.1111/j.1365-2222.2010.03470.x.CrossRefGoogle Scholar
- Sousa, R., Osório, H., Duque, L., Ribeiro, H., Cruz, A., & Abreu, I. (2014). Identification of Plantago lanceolata pollen allergens using an immunoproteomic approach. J Investig Allergol Clin Immunol, 24(3), 177–183.Google Scholar
- Sposato, B., Liccardi, G., RussoM, Folletti I., Siracusa, A., Ventura, M. T., Rolla, G., et al. (2014). Cypress pollen: An unexpected major sensitizing agent in differentregions of Italy. Journal of Investigational Allergology and Clinical Immunology, 24(1), 23–28.Google Scholar
- Subiza J. Pollen counts as a tool for clinical research. In: Basomba A and Sastre J eds. (1995) Postgraduate courses and practical workshops; Syllabus. Valencia ECACI-95, 305–311.Google Scholar
- Tripodi, S., Frediani, T., Lucarelli, S., Macrì, F., Pingitore, G., Di RienzoBusinco, A., et al. (2012). Molecular profiles of IgE to Phleumpratense in children with grass pollen allergy: Implications for specific immunotherapy. Journal of Allergy and Clinical Immunology, 129(3), 834–839. https://doi.org/10.1016/j.jaci.2011.10.045.CrossRefGoogle Scholar
- Valenta, R., Twaroch, T., & Swoboda, I. (2007). Component-resolved diagnosis to optimize allergen-specific immunotherapy in the Mediterranean area. J Investig Allergol Clin Immunol, 17(Suppl 1), 36–40.Google Scholar