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Aerobiologia

pp 1–10 | Cite as

Characterization of Sclerotinia sclerotiorum airborne inoculum, the widespread agent of white mould disease

  • Christel LeyronasEmail author
  • Marc Benigni
  • Stéphane Leignez
  • Magali Duffaud
  • François Villeneuve
  • Philippe C. Nicot
Original Paper
  • 24 Downloads

Abstract

A means to rationalize the use of fungicides for crop protection and to make agriculture friendlier to environment and human health is to develop disease-risk forecasting systems based on the assessment of airborne inoculum abundance. Sclerotinia sclerotiorum, the pandemic agent of white mould disease, is disseminated via the atmosphere in the form of ascospores. These airborne spores are the primary sources of inoculum initiating the majority of epidemics. However, for witloof chicory (Cichorium intybus var. foliosum L.), there is no data about airborne inoculum of S. sclerotiorum, which makes it difficult to develop a forecasting model. In the present study, we characterized the temporal evolution of the abundance and of the genetic characteristics of S. sclerotiorum inoculum on a witloof chicory field located in Northern France over a 3-year period. To our knowledge, this study provides the first quantification of viable airborne populations of this fungus in witloof chicory crops. Moreover, it provides the first genetic characterization of S. sclerotiorum airborne inoculum. The results show that viable ascospores were present through 80% of the sampling dates. A significant correlation between abundance of airborne ascospores and local relative humidity suggests a local origin of inoculum. However, the existence of a slight genetic differentiation between isolates carried by air masses coming from the West and from the North-West is compatible with the hypothesis of a distant origin of S. sclerotiorum inoculum. We discuss the additional studies that are envisioned to clarify the origin of S. sclerotiorum airborne inoculum in witloof chicory fields.

Keywords

Air mass trajectory Ascospores Quantification Microsatellite markers Witloof chicory 

Notes

Acknowledgements

This study was supported in part by a CASDAR grant of the French Ministry of Agriculture (SCLEROLEG Project) and by the Groupement d’Intérêt Scientifique pour la Production Intégrée des Cultures légumières (GIS PIClég).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Christel Leyronas
    • 1
    Email author
  • Marc Benigni
    • 2
  • Stéphane Leignez
    • 2
  • Magali Duffaud
    • 1
  • François Villeneuve
    • 3
  • Philippe C. Nicot
    • 1
  1. 1.Pathologie VégétaleINRAMontfavetFrance
  2. 2.APEFArrasFrance
  3. 3.Ctifl, Centre de LanxadePrigonrieuxFrance

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