Allelopathic effects and potential active substances of Ceratophyllum demersum L. on Chlorella vulgaris Beij.
Morphological responses of green algae to submerged macrophytes were rarely studied, and the potential active substances played roles in the interaction were little known previously. In the current work, according to acetone extract experiments, it was demonstrated that submerged macrophytes (Ceratophyllum demersum L.) could allelopathically inhibit the growth of Chlorella vulgaris Beij. and induce its colony formation, the effects of which were concentration dependent. Gas chromatography–mass spectrometry analysis revealed eight kinds of active substances from the C. demersum extracts, namely hexanoic acid, phthalic acid, octanedioic acid, butenoic acid, azelaic acid, palmitic acid, alpha linolenic acid and pentanedioic acid. Standard compound addition test indicated that palmitic acid and alpha linolenic acid might play important roles in the induction of colony formation and growth inhibition of C. vulgaris. This study provided some more new insights into the photosynthetic organism interaction between submerged macrophytes and green algae, in terms of not only growth but also morphological responses.
KeywordsSubmerged macrophytes Chlorella vulgaris Colony formation Allelopathy Active compounds
This work was supported by the National Nature Science Foundation Project of China (Nos. 31500380 & 31700405). Besides, we really thank the editors and anonymous reviewers’ suggestions on improving the whole manuscript. We are also grateful to our co-author Dr. Yunni Gao for her assistance in the experimentation and manuscript revision.
- Dong J, Chang MY, Li CL, Li JC, Shang XY (2018a) Morphological and growth responses of two green algal strains to toxic Microcystis are dependent on the cultivation growth phase of filtrate and target strain. Ann Limnol Int J Limnol 54(8):70–76Google Scholar
- Gao YN, Liu BY, Xu D, Zhou QH, Hu CY, Ge FJ, Zhang LP, Wu ZB (2011a) Phenolic compounds exuded from two submerged freshwater macrophytes and their allelopathic effects on Microcystis aeruginosa. Pol J Environ Stud 20(5):1153–1159Google Scholar
- Gross EM, Erhard D, Enikö I (2003) Allelopathic activity of Ceratophyllum demersum L. and Najas marina spp. Intermedia (Wolfgang) Casper. Hydrobiologia 506-509(1-3): 583-589Google Scholar
- Hong Y, Hu HY (2007) Effects of the aquatic extracts of Arundo donax L. on the growth of freshwater algae. Allelopath J 20(2):315–326Google Scholar
- Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV–VIS spectroscopy. In: Wrolstad RE, Acree TE, An H, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Sporns P (eds) Current protocols in food analytical chemistry. Wiley, London, pp F4.3.1–F4.3.8Google Scholar
- Wang HQ, Zhu HJ, Zhang LY, Xue WJ, Yuan B (2014) Identification of antialgal compounds from the aquatic plant Elodea nuttallii. Allelopath J 34(2):207–213Google Scholar
- Xian QM, Chen HD, Zou HX, Yin DQ (2004) Analysis of organic acids in aqueous leachates of three submerged macrophytes. J Plant Resour Environ 13(3):57–58 (in Chinese) Google Scholar
- Yasumoto M, Ooi T, Takenori K, Kasai F (2000) Characterization of Daphnia kairomone inducing morphological change of green alga Actinastrum sp. Tennen Yuki Kagobutsu Toronkai Keon Yoshishu 42:385–390Google Scholar
- Zuo SP, Wan K, Ma SM, Ye LT (2014) Combined allelopathic potential of aquatic plants species to control algae. Allelopath J 2:315–323Google Scholar