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Design of an Adhesive Film-Based Microfluidic Device for Alginate Hydrogel-Based Cell Encapsulation

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Abstract

To support the increasing translational use of transplanted cells, there is a need for high-throughput cell encapsulation technologies. Microfluidics is a particularly promising candidate technology to address this need, but conventional polydimethylsiloxane devices have encountered challenges that have limited their utility, including clogging, leaking, material swelling, high cost, and limited scalability. Here, we use a rapid prototyping approach incorporating patterned adhesive thin films to develop a reusable microfluidic device that can produce alginate hydrogel microbeads with high-throughput potential for microencapsulation applications. We show that beads formed in our device have high sphericity and monodispersity. We use the system to demonstrate effective cell encapsulation of mesenchymal stem cells and show that they can be maintained in culture for at least 28 days with no measurable reduction in viability. Our approach is highly scalable and will support diverse translational applications of microencapsulated cells.

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Acknowledgments

The authors would like to acknowledge the support of Michael Hunckler, Michael LeCompte, and Paige Brabant for their technical help on this project. K.E. acknowledges support from the NIH T32 Training Program entitled Studies in Translational Regenerative Medicine (EB014836-01A1).

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Correspondence to Emmanuel C. Opara.

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Enck, K., Rajan, S.P., Aleman, J. et al. Design of an Adhesive Film-Based Microfluidic Device for Alginate Hydrogel-Based Cell Encapsulation. Ann Biomed Eng (2020) doi:10.1007/s10439-020-02453-9

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Keywords

  • Alginate
  • Microencapsulation
  • Stem cell
  • Cell transplantation