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Annals of Biomedical Engineering

, Volume 47, Issue 5, pp 1281–1290 | Cite as

Probing the Depth of the Myocardium: Vasculature, Transit Time, and Perfusion Within the Left Ventricular Wall

  • Erik L. RitmanEmail author
  • A. J. Vercnocke
  • M. Zamir
Article

Abstract

The branching architecture of arterial trees traversing the thickness of the left ventricular wall is studied to determine the way in which adequate blood supply is provided to myocardial tissue at different depths within the wall thickness from arterial trees originating at the epicardial surface. The study is based on micro-CT images of tissue biopsies, coupled with a dedicated vascular tree analysis program. The results show that this combination of methodologies allows a more detailed and much more accurate exploration of the vasculature within the sampled tissue than is possible by histological means. The spatial density of the smallest resolvable “end” arterioles is found to be higher in the sub-endocardial region than in the sub-epicardial region, with vascular branching architecture consistent with a fractal structure. The concept of “transit time” is introduced as an approximate measure of the time it takes bulk flow to reach different regions of the myocardium. Our data suggest that a transit time differential is a major contributor to the equalization of transmural perfusion gradient against unequal distribution of “end’ arteriolar density.

Keywords

Myocardial perfusion Vascular trees Microvasculature Terminal arterioles Transit time Fractals 

Notes

ACKNOWLEDGMENTS

This work was funded in part by National Institutes of Health grant, R021 HL-117359. Special thanks to Ms. J. L. Anderson and Mr. S. M. Jorgensen for their performance of the animal experiment and micro-CT scanning as well as Ms. D. C. Darling for assembling and formatting this manuscript.

Conflict of Interest

There are no conflict of interests with any one of the authors.

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Copyright information

© Biomedical Engineering Society 2019

Authors and Affiliations

  1. 1.Department of Physiology and Biomedical EngineeringMayo Clinic College of Medicine and ScienceRochesterUSA
  2. 2.Department of RadiologyMayo Clinic College of Medicine and ScienceRochesterUSA
  3. 3.Departments of Applied Mathematics and of Medical BiophysicsThe University of Western OntarioLondonCanada

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