Label-Free Sensing and Classification of Old Stored Blood
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Transfusion is crucial in surgical operation and anemia treatment. However, several hemorheological properties of blood are adversely altered during blood storage. After transfusion, these adverse alterations are related with decrease of oxygen and ion transport in blood circulation, which increase the mortality of transfused patients. Therefore, accurate sensing of whether a blood supply is still viable for transfusion or not is extremely important. In this study, a H-shaped microfluidic device and digital in-line holographic microscopy were employed to measure temporal variations of blood viscosity and the optical focusing property of erythrocytes during blood storage. Stored rat blood samples separately preserved in citrate phosphate dextrose adenine-1 (CPDA-1) and ethylenediaminetetraacetic acid (EDTA) underwent considerable changes in their biophysical parameters after 2 weeks. Compared with EDTA, CPDA-1 preserves the hemorheological properties of stored blood more effectively. We propose new criteria for depository period of stored blood and indexes, such as viscosity and focal length of erythrocytes, to determine its appropriateness for transfusion. These criteria and indexes can be effectively used for high-throughput prescreening to reduce the risk of transfusion of aged blood or diagnose hematological diseases.
KeywordsTransfusion Blood screening Microfluidics Holographic microscopy Viscosity Light scattering pattern
The authors thank to H.W. Park of POSTECH for advice on setting up the procedures for rat blood storage and treatments.
Conflict of interest
The authors declare that they have no competing interests and all authors participated sufficiently in the work.
Supplementary Video (MP4 6102 kb)
Supplementary video S1: The measurement procedure of viscosity using the microfluidic device. The amount of blood sample that diverged through the junction channel was decreased with the increase of flow rate of the reference fluid. When both of the fluids reached the hydrostatic force balance, flow reversal of the test fluid in the junction channel occurred. Then, the viscosity of the blood was derived by using the simplified flow ratio equation
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