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Annals of Biomedical Engineering

, Volume 45, Issue 4, pp 884–897 | Cite as

Full Mimicking of Coronary Hemodynamics for Ex-Vivo Stimulation of Human Saphenous Veins

  • Marco PiolaEmail author
  • Matthijs Ruiter
  • Riccardo Vismara
  • Valeria Mastrullo
  • Marco Agrifoglio
  • Marco Zanobini
  • Maurizio Pesce
  • Monica Soncini
  • Gianfranco Beniamino Fiore
Article

Abstract

After coronary artery bypass grafting, structural modifications of the saphenous vein wall lead to lumen narrowing in response to the altered hemodynamic conditions. Here we present the design of a novel ex vivo culture system conceived for mimicking central coronary artery hemodynamics, and we report the results of biomechanical stimulation experiments using human saphenous vein samples. The novel pulsatile system used an aortic-like pressure for forcing a time-dependent coronary-like resistance to obtain the corresponding coronary-like flow rate. The obtained pulsatile pressures and flow rates (diastolic/systolic: 80/120 mmHg and 200/100 mL/min, respectively) showed a reliable mimicking of the complex coronary hemodynamic environment. Saphenous vein segments from patients undergoing coronary artery bypass grafting (n = 12) were subjected to stimulation in our bioreactor with coronary pulsatile pressure/flow patterns or with venous-like perfusion. After 7-day stimulation, SVs were fixed and stained for morphometric evaluation and immunofluorescence. Results were compared with untreated segments of the same veins. Morphometric and immunofluorescence analysis revealed that 7 days of pulsatile stimulation: (i) did not affect integrity of the vessel wall and lumen perimeter, (ii) significantly decreased both intima and media thickness, (iii) led to partial endothelial denudation, and (iv) induced apoptosis in the vessel wall. These data are consistent with the early vessel remodeling events involved in venous bypass adaptation to arterial flow/pressure patterns. The pulsatile system proved to be a suitable device to identify ex vivo mechanical cues leading to graft adaptation.

Keywords

Coronary flow rate Pulsatile pressure Saphenous vein graft disease Ex vivo platform Wall remodeling 

Notes

Acknowledgments

This work was supported by the Italian Ministry of Health research Project RF-2011-02346867. The authors would like to thank Dr. Emilio Savoldelli for his support during the preliminary design of the CPD circuit and Dr. Francesco Sturla for his support with MATLAB.

Disclosure

The authors declare no conflict of interest to disclose.

Supplementary material

10439_2016_1747_MOESM1_ESM.pdf (611 kb)
Supplementary material 1 (PDF 612 kb)
10439_2016_1747_MOESM2_ESM.png (46 kb)
Supplementary Figure S1. A) Schematic of the RC hydraulic filter introduced for damping the peristaltic pump pulsations. The flow rate generated by the pump (Q pump ) is filtered by the RC hydraulic filter in order to obtain a quasi-steady flow rate (Q in ) (PNG 46 kb)
10439_2016_1747_MOESM3_ESM.pdf (54 kb)
Supplementary Table S1 (PDF 55 kb)

Supplementary Video S1. This video describes the CPD-equipped EVCS during conditioning of human SV in the incubator (MP4 27,382 kb)

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Copyright information

© Biomedical Engineering Society 2016

Authors and Affiliations

  • Marco Piola
    • 1
    Email author
  • Matthijs Ruiter
    • 2
  • Riccardo Vismara
    • 1
  • Valeria Mastrullo
    • 2
  • Marco Agrifoglio
    • 3
  • Marco Zanobini
    • 4
  • Maurizio Pesce
    • 2
  • Monica Soncini
    • 1
  • Gianfranco Beniamino Fiore
    • 1
  1. 1.Dipartimento di Elettronica, Informazione e BioingegneriaPolitecnico di MilanoMilanItaly
  2. 2.Unità di Ingegneria TissutaleCentro Cardiologico Monzino-IRCCSMilanItaly
  3. 3.Dipartimento di Scienze Cliniche e di ComunitàUniversità di MilanoMilanItaly
  4. 4.Divisione di CardiochirurgiaCentro Cardiologico Monzino-IRCCSMilanItaly

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