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Video-based Gait Analysis for Functional Evaluation of Healing Achilles Tendon in Rats

Abstract

Video-based walking track systems have been developed for gait analysis in rat models. However, there is no previous study using video-based tracking systems to address the gait parameters to evaluate the recovery of Achilles tendon rupture models. This study conducted a comprehensive gait analysis using a video-based image processing system. Eighteen Sprague-Dawley rats were randomly assigned to one of three interventional conditions: sham surgery, Achilles tendon repair, and Achilles tendon defect. After surgery, all animals were evaluated using a video-based walking track system. The gait parameters and the Achilles functional index (AFI) were further analyzed. The ankle joint angles of the injury side at mid-stance and pre-swing were highly correlated with the AFI. However, lack of sensitivity was found for the AFI. Increased measurement sensitivity of the Achilles tendon healing condition was found in the ankle joint angle of the involved side at the pre-swing and the level of asymmetry of the hindlimb joint position and stance/swing time. The overall sensitivity of the ankle motion analysis was significantly higher than that of AFI. We conclude that the ankle motion analysis is a reliable, reproducible, and sensitive tool for Achilles tendon analysis in rats.

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Abbreviations

AFI:

Achilles functional index

PL/NPL/EPL:

PL: print length; N: control side; E: experimental side

TS/NTS/ETS:

TS: toe-spread length; N: control side; E: experimental side

IT/NIT/EIT:

IT: intermediary toe-spread length; N: control side; E: experimental side

PLF:

Print length factor

TSF:

Toe-spread factor

ITF:

Intermediary toe-spread factor

AI:

Asymmetry index

References

  1. 1.

    Amado, S., P. A. Armada-da-Silva, F. Joao, A. C. Mauricio, A. L. Luis, M. J. Simoes, and A. P. Veloso. The sensitivity of two-dimensional hindlimb joint kinematics analysis in assessing functional recovery in rats after sciatic nerve crush. Behav. Brain Res. 225:562–573, 2011.

  2. 2.

    Bring, D. K., A. Kreicbergs, P. A. Renstrom, and P. W. Ackermann. Physical activity modulates nerve plasticity and stimulates repair after Achilles tendon rupture. J. Orthop. Res. 25:164–172, 2007.

  3. 3.

    Couto, P. A., V. M. Filipe, L. G. Magalhaes, J. E. Pereira, L. M. Costa, P. Melo-Pinto, J. Bulas-Cruz, A. C. Mauricio, S. Geuna, and A. S. Varejao. A comparison of two-dimensional and three-dimensional techniques for the determination of hindlimb kinematics during treadmill locomotion in rats following spinal cord injury. J. Neurosci. Methods 173:193–200, 2008.

  4. 4.

    de Ruiter, G. C., R. J. Spinner, A. O. Alaid, A. J. Koch, H. Wang, M. J. Malessy, B. L. Currier, M. J. Yaszemski, K. R. Kaufman, and A. J. Windebank. Two-dimensional digital video ankle motion analysis for assessment of function in the rat sciatic nerve model. J. Peripher. Nerv. Syst. 12:216–222, 2007.

  5. 5.

    Don, R., A. Ranavolo, A. Cacchio, M. Serrao, F. Costabile, M. Iachelli, F. Camerota, M. Frascarelli, and V. Santilli. Relationship between recovery of calf-muscle biomechanical properties and gait pattern following surgery for achilles tendon rupture. Clin. Biomech. (Bristol, Avon) 22:211–220, 2007.

  6. 6.

    Filipe, V. M., J. E. Pereira, L. M. Costa, A. C. Mauricio, P. A. Couto, P. Melo-Pinto, and A. S. Varejao. Effect of skin movement on the analysis of hindlimb kinematics during treadmill locomotion in rats. J. Neurosci. Methods 153:55–61, 2006.

  7. 7.

    Fu, S. C., K. M. Chan, L. S. Chan, D. T. Fong, and P. Y. Lui. The use of motion analysis to measure pain-related behaviour in a rat model of degenerative tendon injuries. J. Neurosci. Methods 179:309–318, 2009.

  8. 8.

    Gouwanda, D., and S. M. N. A. Senanayake. Identifying gait asymmetry using gyroscopes—a cross-correlation and Normalized Symmetry Index approach. J. Biomech. 44:972–978, 2011.

  9. 9.

    Krahe, M. A., and G. C. Berlet. Achilles tendon ruptures, re rupture with revision surgery, tendinosis, and insertional disease. Foot Ankle Clin. 14:247–275, 2009.

  10. 10.

    Leppilahti, J., J. Puranen, and S. Orava. Incidence of Achilles tendon rupture. Acta Orthop. Scand. 67:277–279, 1996.

  11. 11.

    Murrell, G. A., E. G. Lilly, H. Davies, T. M. Best, R. D. Goldner, and A. V. Seaber. The Achilles functional index. J. Orthop. Res. 10:398–404, 1992.

  12. 12.

    Ng, C. O., G. Y. Ng, E. K. See, and M. C. Leung. Therapeutic ultrasound improves strength of achilles tendon repair in rats. Ultrasound Med. Biol. 29:1501–1506, 2003.

  13. 13.

    O’Brien, M. The anatomy of the Achilles tendon. Foot Ankle Clin. 10:225–238, 2005.

  14. 14.

    Robinson, R. O., W. Herzog, and B. M. Nigg. Use of force platform variables to quantify the effects of chiropractic manipulation on gait symmetry. J. Manipulative Physiol. Ther. 10:172–176, 1987.

  15. 15.

    See, E. K., G. Y. Ng, C. O. Ng, and D. T. Fung. Running exercises improve the strength of a partially ruptured Achilles tendon. Br. J. Sports Med. 38:597–600, 2004.

  16. 16.

    Smart, G. W., J. E. Taunton, and D. B. Clement. Achilles tendon disorders in runners—a review. Med. Sci. Sports Exerc. 12:231–243, 1980.

  17. 17.

    Yeung, C. K., X. Guo, and Y. F. Ng. Pulsed ultrasound treatment accelerates the repair of Achilles tendon rupture in rats. J. Orthop. Res. 24:193–201, 2006.

  18. 18.

    Yu, P., H. S. Matloub, J. R. Sanger, and P. Narini. Gait analysis in rats with peripheral nerve injury. Muscle Nerve 24:231–239, 2001.

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Acknowledgments

The authors are grateful for the financial support from China Medical University Beigang Hospital, Taiwan.

Author information

Correspondence to Ming-Long Yeh.

Additional information

Associate Editor Thurmon E. Lockhart oversaw the review of this article.

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Liang, J., Chen, M., Hsieh, T. et al. Video-based Gait Analysis for Functional Evaluation of Healing Achilles Tendon in Rats. Ann Biomed Eng 40, 2532–2540 (2012) doi:10.1007/s10439-012-0619-z

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Keywords

  • Achilles tendon repair
  • Functional index
  • Gait analysis
  • Ankle motion
  • Joint kinematics
  • Asymmetry index