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Evolution of wax-on-plastic microfluidics for sub-microliter flow dynamics and its application in distance-based assay

  • Ahmad Z. Qamar
  • Gabriel Parker
  • Gary R. Kinsel
  • Mohtashim H. ShamsiEmail author
Research Paper
  • 146 Downloads

Abstract

Plastic substrates are known for their flexibility, optical clearance, toughness, heat resistance, and nonabsorbent properties. Here, we introduce new wax-on-plastic microfluidic platforms, which are responsive to liquid types and concentrations in sub-microliter volume regime based on their flow behavior. Fabrication of the wax-on-plastic microfluidic platforms do not require heating to create stable hydrophobic barriers and liquids can move with a capillary flow down to 0.25 μL volume in the microchannels having 10 μm height. The flow dynamics of the studied fluids in these channels followed the Darcy’s theoretical model, which can be correlated with their flow velocity and viscosity. The flow velocity of the liquid flow was used to estimate glucose in simulated urine within ~ 40 s run-time. The distance-based assay involving relying on flow velocities was used to establish a dynamic range of the glucose in simulated urine with limit of quantitation of 0.018%, which is almost 3× lower than the lower limit of glucose quantity normally present in diabetic patients, i.e., 0.05–0.1%. Moreover, these microchannels can also work distinctly with various biofluids, such as sweat, urine, and fat-free milk.

Notes

Funding

This work was funded by startup grant from the Southern Illinois University Carbondale.

Supplementary material

10404_2019_2249_MOESM1_ESM.docx (80 kb)
Supplementary material 1 (DOCX 80 kb)

Supplementary material 2 (MOV 42400 kb)

References

  1. Abe K, Suzuki K, Citterio D (2008) Inkjet-printed microfluidic multianalyte chemical sensing paper. Anal Chem 80(18):6928–6934CrossRefGoogle Scholar
  2. Asghar W, Yuksekkaya M, Shafiee H, Zhang M, Ozen MO, Inci F, Kocakulak M, Demirci U (2016) Engineering long shelf life multilayer biologically active surfaces on microfluidic devices for point of care applications. Sci Rep UK 6:1–10CrossRefGoogle Scholar
  3. Ballerini DR, Li X, Shen W (2012) Patterned paper and alternative materials as substrates for low-cost microfluidic diagnostics. Microfluid Nanofluid 13(5):769–787CrossRefGoogle Scholar
  4. Betancur V, Sun JB, Wu NQ, Liu YX (2017) Integrated lateral flow device for flow control with blood separation and biosensing. Micromachines 8(12):1–19Google Scholar
  5. Bruzewicz DA, Reches M, Whitesides GM (2008) Low-cost printing of poly(dimethylsiloxane) barriers to define microchannels in paper. Anal Chem 80(9):3387–3392CrossRefGoogle Scholar
  6. Carrilho E, Martinez AW, Whitesides GM (2009) Understanding wax printing: a simple micropatterning process for paper-based microfluidics. Anal Chem 81(16):7091–7095CrossRefGoogle Scholar
  7. Cate DM, Dungchai W, Cunningham JC, Volckens J, Henry CS (2013) Simple, distance-based measurement for paper analytical devices. Lab Chip 13(12):2397–2404CrossRefGoogle Scholar
  8. Chatterjee D, Mansfield DS, Woolley AT (2014) Microfluidic devices for label-free and non-instrumented quantitation of unamplified nucleic acids by flow distance measurement. Anal Methods UK 6(20):8173–8179CrossRefGoogle Scholar
  9. Chen Y, Chu WR, Liu W, Guo XY (2018) Distance-based carcinoembryonic antigen assay on microfluidic paper immunodevice. Sensor Actuat B-Chem 260:452–459CrossRefGoogle Scholar
  10. Cummins BM, Chinthapatla R, Ligler FS, Walker GM (2017) Time-dependent model for fluid flow in porous materials with multiple pore sizes. Anal Chem 89(8):4377–4381CrossRefGoogle Scholar
  11. DeFronzo RA, Davidson JA, Del Prato S (2012) The role of the kidneys in glucose homeostasis: a new path towards normalizing glycaemia. Diabetes Obes Metab 14(1):5–14CrossRefGoogle Scholar
  12. Dungchai W, Chailapakul O, Henry CS (2011) A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing. Analyst 136(1):77–82CrossRefGoogle Scholar
  13. Farkas T, Zhong GM, Guiochon G (1999) Validity of Darcy’s law at low flow-rates in liquid chromatography. J Chromatogr A 849(1):35–43CrossRefGoogle Scholar
  14. Free AH, Adams EC, Kercher ML, Free HM, Cook MH (1957) Simple specific test for urine glucose. Clin Chem 3(3):163–168Google Scholar
  15. Gerold CT, Bakker E, Henry CS (2018) Selective distance-based K + quantification on paper-based microfluidics. Anal Chem 90(7):4894–4900CrossRefGoogle Scholar
  16. Hong S, Kim W (2015) Dynamics of water imbibition through paper channels with wax boundaries. Microfluid Nanofluid 19(4):845–853CrossRefGoogle Scholar
  17. Hossain MF, Park JY (2016) Plain to point network reduced graphene oxide-activated carbon composites decorated with platinum nanoparticles for urine glucose detection. Sci Rep 6:21009CrossRefGoogle Scholar
  18. Jang I, Song S (2015) Facile and precise flow control for a paper-based microfluidic device through varying paper permeability. Lab Chip 15(16):3405–3412CrossRefGoogle Scholar
  19. Jenkins G, Wang Y, Xie YL, Wu Q, Huang W, Wang LH, Yang X (2015) Printed electronics integrated with paper-based microfluidics: new methodologies for next-generation health care. Microfluid Nanofluid 19(2):251–261CrossRefGoogle Scholar
  20. Jin S-Q, Guo S-M, Zuo P, Ye B-C (2015) A cost-effective Z-folding controlled liquid handling microfluidic paper analysis device for pathogen detection via ATP quantification. Biosens Bioelectron 63:379–383CrossRefGoogle Scholar
  21. Kalish B, Luong J, Roper J, Beaudette C, Tsutsui H (2017) Distance-based Quantitative DNA Detection in a Paper-based Microfluidic Device. In: 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (Nems), pp 337–341Google Scholar
  22. Koivunen R, Jutila E, Bollstrom R, Gane P (2016) Hydrophobic patterning of functional porous pigment coatings by inkjet printing. Microfluid Nanofluid 20(6):1–21CrossRefGoogle Scholar
  23. Kuo JS, Chiu DT (2011) Disposable microfluidic substrates: transitioning from the research laboratory into the clinic. Lab Chip 11(16):2656–2665CrossRefGoogle Scholar
  24. Li X, Liu XY (2014) Fabrication of three-dimensional microfluidic channels in a single layer of cellulose paper. Microfluid Nanofluid 16(5):819–827CrossRefGoogle Scholar
  25. Li H, Han D, Pauletti GM, Steckl AJ (2014) Blood coagulation screening using a paper-based microfluidic lateral flow device. Lab Chip 14(20):4035–4041CrossRefGoogle Scholar
  26. Liu CY, Gomez FA (2017) A microfluidic paper-based device to assess acetylcholinesterase activity. Electrophoresis 38(7):1002–1006CrossRefGoogle Scholar
  27. Liu H, Xiang Y, Lu Y, Crooks RM (2012) Aptamer-based origami paper analytical device for electrochemical detection of adenosine. Angew Chem Int Edit 51(28):6925–6928CrossRefGoogle Scholar
  28. Lu Y, Shi WW, Qin JH, Lin BC (2010) Fabrication and characterization of paper-based microfluidics prepared in nitrocellulose membrane by wax printing. Anal Chem 82(1):329–335CrossRefGoogle Scholar
  29. Montgomery RH, Phelan K, Stone SD, Decuir F, Hollins BC (2018) Photolithography-free PDMS stamps for paper microdevice fabrication. Rapid Prototyp J 24(2):361–367CrossRefGoogle Scholar
  30. Nge PN, Rogers CI, Woolley AT (2013) Advances in microfluidic materials, functions, integration, and applications. Chem Rev 113(4):2550–2583CrossRefGoogle Scholar
  31. Nie ZH, Nijhuis CA, Gong JL, Chen X, Kumachev A, Martinez AW, Narovlyansky M, Whitesides GM (2010a) Electrochemical sensing in paper-based microfluidic devices. Lab Chip 10(4):477–483CrossRefGoogle Scholar
  32. Nie ZH, Deiss F, Liu XY, Akbulut O, Whitesides GM (2010b) Integration of paper-based microfluidic devices with commercial electrochemical readers. Lab Chip 10(22):3163–3169CrossRefGoogle Scholar
  33. Nilghaz A, Ballerini DR, Fang XY, Shen W (2014) Semiquantitative analysis on microfluidic thread-based analytical devices by ruler. Sensor Actuat B-Chem 191:586–594CrossRefGoogle Scholar
  34. Qamar AZ, Amar K, Kohli P, Chowdhury F, Shamsi MH (2016) Wax patterned microwells for stem cell fate study. RSC Adv 6(106):104919–104924CrossRefGoogle Scholar
  35. Rackus DG, Shamsi MH, Wheeler AR (2015) Electrochemistry, biosensors and microfluidics: a convergence of fields. Chem Soc Rev 44(15):5320–5340CrossRefGoogle Scholar
  36. Sechi D, Greer B, Johnson J, Hashemi N (2013) Three-dimensional paper-based microfluidic device for assays of protein and glucose in urine. Anal Chem 85(22):10733–10737CrossRefGoogle Scholar
  37. Song SH, Lim CS, Shin S (2013) Migration distance-based platelet function analysis in a microfluidic system. Biomicrofluidics 7(6):1–10Google Scholar
  38. Tokel O, Yildiz UH, Inci F, Durmus NG, Ekiz OO, Turker B, Cetin C, Rao S, Sridhar K, Natarajan N, Shafiee H, Dana A, Demirci U (2015) Portable microfluidic integrated plasmonic platform for pathogen detection. Sci Rep UK 5:1–9Google Scholar
  39. Trantidou T, Elani Y, Parsons E, Ces O (2017) Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition. Microsyst Nanoeng 3:1–9CrossRefGoogle Scholar
  40. Urakami T, Suzuki J, Yoshida A, Saito H, Mugishima H (2008) Incidence of children with slowly progressive form of type 1 diabetes detected by the urine glucose screening at schools in the Tokyo Metropolitan Area. Diabetes Res Clin Pract 80(3):473–476CrossRefGoogle Scholar
  41. Walsh DI, Kong DS, Murthy SK, Carr PA (2017) Enabling microfluidics: from clean rooms to makerspaces. Trends Biotechnol 35(5):383–392CrossRefGoogle Scholar
  42. Wang S, Ge L, Song X, Yu J, Ge S, Huang J, Zeng F (2012) Based chemiluminescence ELISA: lab-on-paper based on chitosan modified paper device and wax-screen-printing. Biosens Bioelectron 31(1):212–218CrossRefGoogle Scholar
  43. Wang Y, Luo JP, Liu JT, Li XR, Kong Z, Jin HY, Cai XX (2018) Electrochemical integrated paper-based immunosensor modified with multi-walled carbon nanotubes nanocomposites for point-of-care testing, of 17 beta-estradiol. Biosens Bioelectron 107:47–53CrossRefGoogle Scholar
  44. Wei XF, Tian T, Jia SS, Zhu Z, Ma YL, Sun JJ, Lin ZY, Yang CJ (2016) microfluidic distance readout sweet hydrogel integrated paper based analytical Device (mu DiSH-PAD) for visual quantitative point-of-care testing. Anal Chem 88(4):2345–2352CrossRefGoogle Scholar
  45. Wu L, Ma C, Zheng X, Liu H, Yu J (2015) based electrochemiluminescence origami device for protein detection using assembled cascade DNA–carbon dots nanotags based on rolling circle amplification. Biosens Bioelectron 68:413–420CrossRefGoogle Scholar
  46. Xie Y, Wei XF, Yang QZ, Guan ZC, Liu D, Liu X, Zhou LJ, Zhu Z, Lin ZY, Yang CY (2016) A Shake&Read distance-based microfluidic chip as a portable quantitative readout device for highly sensitive point-of-care testing. Chem Commun 52(91):13377–13380CrossRefGoogle Scholar
  47. Cai LF, Fang YL, Mo YH, Huang YS, Xu CX, Zhang Z, Wang MX (2017) Visual quantification of Hg on a microfluidic paper-based analytical device using distance-based detection technique. AIP Adv 7(8):1–8CrossRefGoogle Scholar
  48. Yamada K, Citterio D, Henry CS (2018) “Dip-and-read” paper-based analytical devices using distance-based detection with color screening. Lab Chip 18(10):1485–1493CrossRefGoogle Scholar
  49. Zhang H, Hu XJ, Fu X (2014) Aptamer-based microfluidic beads array sensor for simultaneous detection of multiple analytes employing multienzyme-linked nanoparticle amplification and quantum dots labels. Biosens Bioelectron 57:22–29CrossRefGoogle Scholar
  50. Zhang Y, Zuo P, Ye B-C (2015) A low-cost and simple paper-based microfluidic device for simultaneous multiplex determination of different types of chemical contaminants in food. Biosens Bioelectron 68:14–19CrossRefGoogle Scholar
  51. Zhu Z, Guan ZC, Jia SS, Lei ZC, Lin SC, Zhang HM, Ma YL, Tian ZQ, Yang CJ (2014) Au@Pt Nanoparticle Encapsulated Target-Responsive Hydrogel with Volumetric Bar-Chart Chip Readout for Quantitative Point-of-Care Testing. Angew Chem Int Edit 53(46):12503–12507Google Scholar
  52. Zuk RF, Ginsberg VK, Houts T, Rabbie J, Merrick H, Ullman EF, Fischer MM, Sizto CC, Stiso SN, Litman DJ (1985) Enzyme immunochromatography—a quantitative immunoassay requiring no instrumentation. Clin Chem 31(7):1144–1150Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistrySouthern Illinois University CarbondaleCarbondaleUSA

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