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A fixed cytometer chip for identification of cell populations and real-time monitoring of single-cell apoptosis under gradient UV radiation

  • Anyue Xia
  • Mingzhe GanEmail author
  • Huan Xu
  • Yiheng Zhang
  • Dandan Wang
  • Jing Du
  • Qian Sun
  • Jiana Jiang
  • Dan Luo
  • Jinhui CuiEmail author
  • Peifeng LiuEmail author
Research Paper
  • 91 Downloads

Abstract

Cytometry is a basic method to determine cell populations and morphology. Flow cytometry and hemocytometry are the two most common methods among cytometric technologies. However, flow cytometry needs bulky and expensive equipment as well as professional operations, while hemocytometry is limited by its simple function. Both of them are not suitable for real-time monitoring of the morphological changes of single cells. Here, we developed a fixed cytometer chip with two functional modes for both identification of cell populations (I-mode) and real-time monitoring of single-cell morphological changes (M-mode). In I-mode, the fixed cytometer chip was employed to evaluate the cell populations, the results were in accordance with those from the hemocytometer counting and flow cytometry. Besides that, the cell populations were further precisely identified by measuring two-color fluorescence intensities of single cells, which were consistent with the dual parameter analysis in flow cytometry. In M-mode, the chip was applied to real-time monitoring of the single-cell apoptosis under gradient UV radiation, generated by a novel stair-like UV shield. The dynamic apoptotic morphologies of a large number of single cells were monitored in real-time by time-lapse imaging. In addition, we integrated eight parallel channels on a 60 mm × 30 mm chip, and the chip could achieve scalable single-cell capture and analysis capability. This fixed cytometer chip is bifunctional, easy-to-handle, universal, and scalable.

Keywords

Cytometer chip Cell population identification Real-time monitoring Cell Apoptosis Gradient UV radiation 

Notes

Acknowledgements

We gratefully acknowledge the financial support from the National Natural Science Foundation of China (81771968, 81472842, 21778071, and 31400087), Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (20181705), Shanghai Talent Development Fund (2017053), Translational Medicine Cross Research Grant of Shanghai Jiao Tong University (ZH2018ZDA05), Youth Innovation Promotion Association CAS and Suzhou Institute of Nano-Tech and Nano-Bionics Owned Fund (Y5AAS11001).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10404_2019_2244_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1345 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Central Laboratory, Renji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  3. 3.Micro-Nano Research and Diagnosis Center, Renji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  4. 4.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhouChina
  5. 5.Department of Clinical Laboratory Medicine, Southwest HospitalArmy Medical University (Third Military Medical University)ChongqingChina
  6. 6.Kavli Institute at Cornell for Nanoscale ScienceCornell UniversityIthacaUSA
  7. 7.Department of Biological and Environmental EngineeringCornell UniversityIthacaUSA

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