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Dean-flow-coupled elasto-inertial particle and cell focusing in symmetric serpentine microchannels

  • Dan Yuan
  • Ronald Sluyter
  • Qianbin Zhao
  • Shiyang Tang
  • Sheng Yan
  • Guolin Yun
  • Ming Li
  • Jun ZhangEmail author
  • Weihua LiEmail author
Research Paper
  • 181 Downloads
Part of the following topical collections:
  1. Particle motion in non-Newtonian microfluidics

Abstract

This work investigates particle focusing under Dean-flow-coupled elasto-inertial effects in symmetric serpentine microchannels. A small amount of polymers were added to the sample solution to tune the fluid elasticity, and allow particles to migrate laterally and reach their equilibriums at the centerline of a symmetric serpentine channel under the synthesis effect of elastic, inertial and Dean-flow forces. First, the effects of the flow rates on particle focusing in viscoelastic fluid in serpentine channels were investigated. Then, comparisons with particle focusing in the Newtonian fluid in the serpentine channel and in the viscoelastic fluid in the straight channel were conducted. The elastic effect and the serpentine channel structure could accelerate the particle focusing as well as reduce the channel length. This focusing technique has the potential as a pre-ordering unit in flow cytometry for cell counting, sorting, and analysis. Moreover, focusing behaviour of Jurkat cells in the viscoelastic fluid in this serpentine channel was studied. Finally, the cell viability in the culture medium containing a dissolved polymer and after processing through the serpentine channel was tested. The polymer within this viscoelastic fluid has a negligible effect on cell viability.

Keywords

Viscoelastic fluid Dean-flow-coupled elasto-inertial effects Viscoelastic force Cell viability 

Notes

Acknowledgements

This work is supported by the National Natural Science Foundation of China (Grant no. 51705257), the Australian Research Council (ARC) Discovery Project (Grant no. DP180100055), and the Natural Science Foundation of Jiangsu Province (Grant no. BK20170839).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

References

  1. Augustsson P, Åberg LB, Swärd-Nilsson A-MK, Laurell T (2009) Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing. Microchim Acta 164:269–277CrossRefGoogle Scholar
  2. Bhagat AAS, Kuntaegowdanahalli SS, Kaval N, Seliskar CJ, Papautsky I (2010) Inertial microfluidics for sheath-less high-throughput flow cytometry. Biomed Microdevices 12:187–195CrossRefGoogle Scholar
  3. Cha S et al (2012) Cell stretching measurement utilizing viscoelastic particle focusing. Anal Chem 84:10471–10477CrossRefGoogle Scholar
  4. Cha S, Kang K, You JB, Im SG, Kim Y, Kim JM (2014) Hoop stress-assisted three-dimensional particle focusing under viscoelastic flow. Rheol Acta 53:927–933CrossRefGoogle Scholar
  5. Crowley TA, Pizziconi V (2005) Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications. Lab Chip 5:922–929CrossRefGoogle Scholar
  6. D’Avino G, Maffettone P (2015) Particle dynamics in viscoelastic liquids. J Non Newtonian Fluid Mech 215:80–104MathSciNetCrossRefGoogle Scholar
  7. D’Avino G, Romeo G, Villone MM, Greco F, Netti PA, Maffettone PL (2012) Single line particle focusing induced by viscoelasticity of the suspending liquid: theory, experiments and simulations to design a micropipe flow-focuser. Lab Chip 12:1638–1645CrossRefGoogle Scholar
  8. D’Avino G, Greco F, Maffettone PL (2017) Particle migration due to viscoelasticity of the suspending liquid and its relevance in microfluidic devices. Annu Rev Fluid Mech 49:341–360MathSciNetCrossRefGoogle Scholar
  9. Davis JA et al (2006) Deterministic hydrodynamics: taking blood apart. Proc Natl Acad Sci USA 103:14779–14784CrossRefGoogle Scholar
  10. Del Giudice F, Sathish S, D’Avino G, Shen AQ (2017) “From the edge to the center”: viscoelastic migration of particles and cells in a strongly shear-thinning liquid flowing in a microchannel. Anal Chem 89:13146–13159CrossRefGoogle Scholar
  11. Di Carlo D (2009) Inertial microfluidics. Lab Chip 9:3038–3046.  https://doi.org/10.1039/B912547G CrossRefGoogle Scholar
  12. Faridi MA, Ramachandraiah H, Banerjee I, Ardabili S, Zelenin S, Russom A (2017) Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics. J Nanobiotechnology 15:3CrossRefGoogle Scholar
  13. Gossett DR et al (2010) Label-free cell separation and sorting in microfluidic systems. Anal Bioanal Chem 397:3249–3267CrossRefGoogle Scholar
  14. Hejazian M, Li W, Nguyen N-T (2015) Lab on a chip for continuous-flow magnetic cell separation. Lab Chip 15:959–970CrossRefGoogle Scholar
  15. Heyman JS (1993) Acoustophoresis separation method. J Acoust Soc America 94(2):1176–1177MathSciNetCrossRefGoogle Scholar
  16. Kang Y, Li D, Kalams SA, Eid JE (2008) DC-Dielectrophoretic separation of biological cells by size. Biomed Microdevices 10:243–249CrossRefGoogle Scholar
  17. Kang K, Lee SS, Hyun K, Lee SJ, Kim JM (2013) DNA-based highly tunable particle focuser. Nat Commun 4:2567CrossRefGoogle Scholar
  18. Khoo BL, Grenci G, Lim YB, Lee SC, Han J, Lim CT (2018) Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device. Nat Protoc 13:34CrossRefGoogle Scholar
  19. Kuntaegowdanahalli SS, Bhagat AAS, Kumar G, Papautsky I (2009) Inertial microfluidics for continuous particle separation in spiral microchannels. Lab Chip 9:2973–2980CrossRefGoogle Scholar
  20. Lee DJ, Brenner H, Youn JR, Song YS (2013) Multiplex particle focusing via hydrodynamic force in viscoelastic fluids. Sci Rep 3:3258CrossRefGoogle Scholar
  21. Leshansky A, Bransky A, Korin N, Dinnar U (2007) Tunable nonlinear viscoelastic “focusing” in a microfluidic device. Phys Rev Lett 98:234501CrossRefGoogle Scholar
  22. Liu C, Ding B, Xue C, Tian Y, Hu G, Sun J (2016) Sheathless focusing and separation of diverse nanoparticles in viscoelastic solutions with minimized shear. Thinning Anal Chem 88:12547–12553CrossRefGoogle Scholar
  23. Liu C et al (2017) Field-free isolation of exosomes from extracellular vesicles by microfluidic viscoelastic flows. ACS Nano 11 6968–6976CrossRefGoogle Scholar
  24. Lu X, Liu C, Hu G, Xuan X (2017) Particle manipulations in non-Newtonian microfluidics: a review. J Colloid Interface Sci 500:182–201CrossRefGoogle Scholar
  25. MacDonald M, Spalding G, Dholakia K (2003) Microfluidic sorting in an optical lattice. Nature 426:421–424CrossRefGoogle Scholar
  26. Magda J, Lou J, Baek S, DeVries K (1991) Second normal stress difference of a Boger. Fluid Polymer 32:2000–2009CrossRefGoogle Scholar
  27. McDonald JC, Whitesides GM (2002) Poly (dimethylsiloxane) as a material for fabricating microfluidic devices. Account Chem Res 35:491–499CrossRefGoogle Scholar
  28. Morton KJ, Loutherback K, Inglis DW, Tsui OK, Sturm JC, Chou SY, Austin RH (2008) Crossing microfluidic streamlines to lyse, label and wash cells. Lab Chip 8:1448–1453CrossRefGoogle Scholar
  29. Nam J, Shin Y, Tan JKS, Lim BY, Lim CT, Kim S (2016) High-throughput malaria parasite separation using a viscoelastic fluid for ultrasensitive PCR detection. Lab Chip 16:2086–2092CrossRefGoogle Scholar
  30. Nitta N et al (2018) Intelligent image-activated cell sorting. Cell 175:266–276.e213CrossRefGoogle Scholar
  31. Pamme N (2007) Continuous flow separations in microfluidic devices. Lab Chip 7:1644–1659CrossRefGoogle Scholar
  32. Pathak JA, Ross D, Migler KB (2004) Elastic flow instability, curved streamlines, and mixing in microfluidic flows. Phys Fluids 16:4028–4034CrossRefGoogle Scholar
  33. Sajeesh P, Sen AK (2014) Particle separation and sorting in microfluidic devices: a review. Microfluid Nanofluid 17:1–52CrossRefGoogle Scholar
  34. Seo KW, Byeon HJ, Huh HK, Lee SJ (2014) Particle migration and single-line particle focusing in microscale pipe flow of viscoelastic fluids. RSC Adv 4:3512–3520CrossRefGoogle Scholar
  35. Sollier E, Murray C, Maoddi P, Di Carlo D (2011) Rapid prototyping polymers for microfluidic devices and high pressure injections. Lab Chip 11:3752–3765CrossRefGoogle Scholar
  36. Vaidyanathan R, Yeo T, Lim CT (2018) Microfluidics for cell sorting and single cell analysis from whole blood. Methods Cell Biol 147:151–173CrossRefGoogle Scholar
  37. Whitesides GM (2006a) The origins and the future of microfluidics. Nature 442:368–373CrossRefGoogle Scholar
  38. Whitesides GM (2006b) The origins and the future of microfluidics. Nature 442:368CrossRefGoogle Scholar
  39. Xiang N, Dai Q, Ni Z (2016a) Multi-train elasto-inertial particle focusing in straight microfluidic channels. Appl Phys Lett 109:134101CrossRefGoogle Scholar
  40. Xiang N, Zhang X, Dai Q, Chen J, Chen K, Ni Z (2016b) Fundamentals of elasto-inertial particle focusing in curved microfluidic channels. Lab Chip 16:2626–2635CrossRefGoogle Scholar
  41. Yamada M, Nakashima M, Seki M (2004) Pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel. Anal Chem 76:5465–5471CrossRefGoogle Scholar
  42. Yan S, Zhang J, Alici G, Du H, Zhu Y, Li W (2014) Isolating plasma from blood using a dielectrophoresis-active hydrophoretic. device Lab Chip 14:2993–3003.  https://doi.org/10.1039/C4LC00343H CrossRefGoogle Scholar
  43. Yang S, Ündar A, Zahn JD (2007) Continuous cytometric bead processing within a microfluidic device for bead based sensing platforms. Lab Chip 7:588–595CrossRefGoogle Scholar
  44. Yang S, Kim JY, Lee SJ, Lee SS, Kim JM (2011) Sheathless elasto-inertial particle focusing and continuous separation in a straight rectangular microchannel. Lab Chip 11:266–273CrossRefGoogle Scholar
  45. Yuan D, Zhang J, Yan S, Pan C, Alici G, Nguyen N-T, Li W (2015) Dean-flow-coupled elasto-inertial three-dimensional particle focusing under viscoelastic flow in a straight channel with asymmetrical expansion–contraction cavity arrays. Biomicrofluidics 9:044108CrossRefGoogle Scholar
  46. Yuan D, Zhang J, Sluyter R, Zhao Q, Yan S, Alici G, Li W (2016a) Continuous plasma extraction under viscoelastic fluid in a straight channel with asymmetrical expansion–contraction cavity arrays. Lab Chip 16:3919–3928CrossRefGoogle Scholar
  47. Yuan D et al (2016b) Investigation of particle lateral migration in sample-sheath flow of viscoelastic fluid newtonian fluid. Electrophoresis 37:2147–2155CrossRefGoogle Scholar
  48. Yuan D et al (2017a) On-chip microparticle and cell washing using co-flow of viscoelastic fluid and Newtonian fluid. Anal Chem 89:9574–9582.  https://doi.org/10.1021/acs.analchem.7b02671 CrossRefGoogle Scholar
  49. Yuan D et al (2017b) Sheathless Dean-flow-coupled elasto-inertial particle focusing and separation in viscoelastic fluid. RSC Adv 7:3461–3469CrossRefGoogle Scholar
  50. Yuan D, Zhao Q, Yan S, Tang S-Y, Alici G, Zhang J, Li W (2018) Recent progress of particle migration in viscoelastic fluids. Lab Chip 18:551–567.  https://doi.org/10.1039/C7LC01076A CrossRefGoogle Scholar
  51. Zeng J, Chen C, Vedantam P, Brown V, Tzeng T-RJ, Xuan X (2012) Three-dimensional magnetic focusing of particles and cells in ferrofluid flow through a straight microchannel. J Micromech Microeng 22:105018CrossRefGoogle Scholar
  52. Zhang J, Yan S, Yuan D, Alici G, Nguyen N-T, Warkiani ME, Li W (2016) Fundamentals and applications of inertial microfluidics: a review. Lab Chip 16:10–34CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Mechanical, Materials, Mechatronic and Biomedical EngineeringUniversity of WollongongWollongongAustralia
  2. 2.School of Biological SciencesUniversity of WollongongWollongongAustralia
  3. 3.Illawarra Health and Medical Research InstituteWollongongAustralia
  4. 4.Department of ChemistryUniversity of TokyoTokyoJapan
  5. 5.School of EngineeringMacquarie UniversitySydneyAustralia
  6. 6.Queensland Micro and Nanotechnology CentreGriffith UniversityBrisbaneAustralia

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