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Top sheath flow-assisted secondary flow particle manipulation in microchannels with the slanted groove structure

  • Qianbin Zhao
  • Dan Yuan
  • Shi-Yang Tang
  • Guolin Yun
  • Sheng Yan
  • Jun ZhangEmail author
  • Weihua LiEmail author
Research Paper
  • 165 Downloads
Part of the following topical collections:
  1. 2018 International Conference of Microfluidics, Nanofluidics and Lab-on-a-Chip, Beijing, China

Abstract

In the regime of inertial microfluidics, the secondary flow is widely adopted to reduce the number of the equilibrium position and improve the focusing performance of particles. At the same time, secondary flow can also enhance the mixing effect and may deteriorate particle focusing due to the induced rotating streams, especially for the particle with a small size. In a double-layered microchannel with slanted groove structures, it has been demonstrated that the generated secondary flow at a high flow rate could focus the particle with a large size (> 8 µm). However, the manipulation of small-size particles (< 8 µm) was unsuccessful as the effects of secondary flow on the small-size particles were not strong enough. In this work, to manipulate the small-size particle, we proposed a scheme to utilize a top sheath flow to enhance the focusing efficiency of the structure-induced secondary flow. The effects of the total flow rate and the flow rate ratio between the sheath and sample flow were investigated comprehensively in a large range. The 4.8 µm particle could be manipulated effectively at different flow rates with the assistance of appropriate sheath flows. Besides, the effects of other factors, such as the quantity of the expansion groove structure, and particle concentration and size, on particle focusing performance were also investigated. We found that the particles with the diameter of 2.9 µm can also be effectively focused within the double-layered microchannel. Furthermore, we demonstrated the continuous plasma extraction from the undiluted whole blood using this proposed technique to validate its potential biological applications. The results show that the purity of plasma extracted could reach up to ~ 99% after a single process. As such, this demonstrated that sheath flow-assisted particle manipulating method can overcome the limitations of conventional design and offer much better performance for controlling smaller particles. Such a platform may enable great potential in the applications of biological and diagnostic assays, for bioparticles smaller than the normal cell size.

Notes

Acknowledgements

J. Zhang greatly acknowledges the support from the National Natural Science Foundation of China (Grant no. 51705257), the Natural Science Foundation of Jiangsu Province (Grant no. BK20170839), and the Griffith University-Peking University Collaborative Travel Grant (036 Research Internal). W. Li acknowledges the support from Australian Research Council Discovery Project (Grant no. 180100055). This work is partially supported by UOW-CSC Scholarship and Dr. Sheng Yan is the recipient of JSPS postdoctoral fellowship.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Mechanical, Materials, Mechatronic and Biomedical EngineeringUniversity of WollongongWollongongAustralia
  2. 2.Department of ChemistryUniversity of TokyoTokyoJapan
  3. 3.Queensland Micro and Nanotechnology CentreGriffith UniversityBrisbaneAustralia

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