Virus concentration and purification by a microfluidic filtering system with an integrated PEGylated antifouling membrane
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We present the integration of a nanoporous membrane functionalized with hydrophilic polymer brushes, poly(ethylene glycol) methacrylate (PEGMA), into a microfluidic device. In order to prevent damage to functional groups on the membrane surfaces, the PEGMA-modified membrane was directly bonded using an intermediate UV-curable adhesive layer by employing the modified “Stamp-and-Stick (SAS)” method and operated at high pressures. We demonstrated that the degree of flux recovery (RFR) of the functionalized membrane during testing with bovine serum albumin, that reached 93% after a cleaning process while unmodified membranes showed only 63% of RFR. The viral particle recovery efficiency and concentration factor were 60.41% and 6.04, respectively.
KeywordsSample preparation Microfluidic diagnostic platforms Membrane separation
This work was supported by Lawrence Livermore National Laboratory and the Defense Advanced Research Planning Agency. Lawrence Livermore National Laboratory is operated by Lawrence Livermore National Security, LLC, for the U.S. Department of Energy, National Nuclear Security Administration under Contract DE-AC52-07NA27344.
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