Virus concentration and purification by a microfluidic filtering system with an integrated PEGylated antifouling membrane
- 110 Downloads
We present the integration of a nanoporous membrane functionalized with hydrophilic polymer brushes, poly(ethylene glycol) methacrylate (PEGMA), into a microfluidic device. In order to prevent damage to functional groups on the membrane surfaces, the PEGMA-modified membrane was directly bonded using an intermediate UV-curable adhesive layer by employing the modified “Stamp-and-Stick (SAS)” method and operated at high pressures. We demonstrated that the degree of flux recovery (RFR) of the functionalized membrane during testing with bovine serum albumin, that reached 93% after a cleaning process while unmodified membranes showed only 63% of RFR. The viral particle recovery efficiency and concentration factor were 60.41% and 6.04, respectively.
KeywordsSample preparation Microfluidic diagnostic platforms Membrane separation
This work was supported by Lawrence Livermore National Laboratory and the Defense Advanced Research Planning Agency. Lawrence Livermore National Laboratory is operated by Lawrence Livermore National Security, LLC, for the U.S. Department of Energy, National Nuclear Security Administration under Contract DE-AC52-07NA27344.
- Hill VR, Kahler AM, Jothikumar N, Johnson TB, Hahn D, Cromeans TL (2007) Multistate evaluation of an ultrafiltration-based procedure for simultaneous recovery of enteric microbes in 100-liter tap water samples. Appl Environ Microbiol 73:4218–4225. https://doi.org/10.1128/aem.02713-06 CrossRefGoogle Scholar
- Ho NT, Fan A, Klapperich CM, Cabodi M (2012) Sample concentration and purification for point-of-care diagnostics. In: 34th annual international conference of the IEEE EMBS, 28 August–1 September, pp 2396–2399. https://doi.org/10.1109/EMBC.2012.6346446
- Rajal VB, McSwain BS, Thompson DE, Leutenegger CM, Kildare BJ, Wuertz S (2007) Validation of hollow fiber ultrafiltration and real-time PCR using bacteriophage PP7 as surrogate for the quantification of viruses from water samples. Water Res 41:1411–1422. https://doi.org/10.1016/j.watres.2006.12.034 CrossRefGoogle Scholar
- Rutjes SA, Italiaander R, van den Berg H, Lodder WJ, Husman AMD (2005) Isolation and detection of enterovirus RNA from large-volume water samples by using the NucliSens miniMAG system and real-time nucleic acid sequence-based amplification. Appl Environ Microbiol 71:3734–3740. https://doi.org/10.1128/aem.71.7.3734-3740.2005 CrossRefGoogle Scholar
- Su Y, Li C, Zhao W, Shi Q, Wang H, Jiang Z, Zhu S (2008) Modification of polyethersulfone ultrafiltration membranes with phosphorylcholine copolymer can remarkably improve the antifouling and permeation properties. J Memb Sci 322:171–177. https://doi.org/10.1016/j.memsci.2008.05.047 CrossRefGoogle Scholar