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Microfluidic multi-target sorting by magnetic repulsion

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Abstract

In magnetophoresis-based microfluidic systems, the free-flow sorting is achieved by incrementally navigating the magnetic target toward a designated outlet. This is typically enabled using high-gradient magnetic concentrators (HGMCs), axially aligned or slightly slanted with the streaming sample flow. Such axial and incremental magnetic manipulation critically constraints the throughput and the number of targets that can be sorted simultaneously. To overcome these constraints, we present an alternative repulsion-based sorting method. The repulsion force is due that induced, over a limited angular expanse, around a single ferromagnetic wire. The wire is positioned transversally against the focused sample flow. Differentially repelled by the repulsive force, each target deflects from its focused path to follow a ribbon-like trajectory that leads to a spatially addressable outlet. The mediated sorting takes place more rapidly and is confined to the region facing the transversal wire. More importantly, the introduced concept design allows for a throughput that is geometrically scalable with the length of the wire. The functionality of the systems is demonstrated experimentally and numerically to yield the simultaneous and complete multi-target sorting of two and more magnetic beads.

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References

  1. Adams JD, Soh HT (2009) Perspectives on utilizing unique features of microfluidics technology for particle and cell sorting. J Lab Autom 14(6):331–340. https://doi.org/10.1016/j.jala.2009.06.003

  2. Adams JD, Kim U, Soh HT (2008) Multitarget magnetic activated cell sorter. Proc Natl Acad Sci 105(47):18165–18170. https://doi.org/10.1073/pnas.0809795105

  3. Alazzam A, Mathew B, Khashan S (2017) Microfluidic platforms for bio-applications. In Zhang D, Wei B (eds) Advanced mechatronics and MEMS devices II. Springer International Publishing, Switzerland, pp 253–282. https://doi.org/10.1007/978-3-319-32180-6_12. http://link.springer.com/

  4. Eisenträger A, Vella D, Griffiths IM (2014) Particle capture efficiency in a multi-wire model for high gradient magnetic separation. Appl Phys Lett 105(3):033508. https://doi.org/10.1063/1.4890965

  5. Fletcher D (1991) Fine particle high gradient magnetic entrapment. IEEE Trans Magn 27(4):3655–3677. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026192484&partnerID=tZOtx3y1%5Cnhttp://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=102936

  6. Fonnum G et al (2005) Characterisation of dynabeads® by magnetization measurements and Mössbauer spectroscopy. J Magn Magn Mater 293(1):41–47. https://doi.org/10.1016/j.jmmm.2005.01.041

  7. Han K-H, Bruno Frazier A (2004) Continuous magnetophoretic separation of blood cells in microdevice format. J Appl Phys 96(10):5797–5802. http://aip.scitation.org/doi/10.1063/1.1803628

  8. Han K, Frazier AB, Drive A (2005) A microfluidic system for continuous magnetophoretic separation of suspended cells using their native magnetic properties. NSTI Nanotech 1(Dmc):187–190

  9. Hoyos M et al (2000) Study of magnetic particles pulse-injected into an annular SPLITT-like channel inside a quadrupole magnetic field. J Chromatogr A 903(1–2):99–116. http://linkinghub.elsevier.com/retrieve/pii/S0021967300008797

  10. Inglis DW et al (2004) Continuous microfluidic immunomagnetic cell separation. Appl Phys Lett 85(21):5093–5095. http://aip.scitation.org/doi/10.1063/1.1823015

  11. Khashan SA et al (2017a) Microdevice for continuous flow magnetic separation for bioengineering applications. J Micromech Microeng 27(5):055016. http://stacks.iop.org/0960-1317/27/i=5/a=055016?key=crossref.8df3a9a140b98851af479168478f8fb5

  12. Khashan SA et al (2017b) Mixture model for biomagnetic separation in microfluidic systems. J Magn Magn Mater 442:118–127. https://doi.org/10.1016/j.jmmm.2017.06.096

  13. Khashan SA, Furlani EP (2012) Effects of particle–fluid coupling on particle transport and capture in a magnetophoretic microsystem. Microfluid Nanofluidics 12(1–4):565–580. http://link.springer.com/10.1007/s10404-011-0898-y

  14. Khashan SA, Furlani EP (2013) Coupled particle–fluid transport and magnetic separation in microfluidic systems with passive magnetic functionality. J Phys D Appl Phys 46(12):125002. http://stacks.iop.org/0022-3727/46/i=12/a=125002?key=crossref.f265203de92addfb0f62dde8e86da09d

  15. Khashan SA, Furlani EP (2014) Scalability analysis of magnetic bead separation in a microchannel with an array of soft magnetic elements in a uniform magnetic field. Sep Purif Technol 125:311–318. http://link.springer.com/10.1007/s10404-011-0898-y

  16. Khashan SA, Elnajjar E, Haik Y (2011) Numerical simulation of the continuous biomagnetic separation in a two-dimensional channel. Int J Multiphase Flow 37(8):947–955. https://doi.org/10.1016/j.ijmultiphaseflow.2011.05.004

  17. Khashan SA, Alazzam A, Furlani EP (2014) Computational analysis of enhanced magnetic bioseparation in microfluidic systems with flow-invasive magnetic elements. Sci Rep 4(1):5299. http://www.nature.com/articles/srep05299

  18. Khashan SA, Alazzam A, Furlani EP (2015) Computational analysis of enhanced magnetic bioseparation in microfluidic systems with flow-invasive magnetic elements. Sci Rep 4(1):5299. http://www.ncbi.nlm.nih.gov/pubmed/24931437

  19. Kim U, Soh HT (2009) Simultaneous sorting of multiple bacterial targets using integrated dielectrophoretic–magnetic activated cell sorter. Lab Chip 9(16):2313. http://xlink.rsc.org/?DOI=b903950c

  20. Kong TF et al (2011) An efficient microfluidic sorter implementation of double meandering micro striplines for magnetic particles switching. Microfluid Nanofluidics 10(5):1069–1078. http://hdl.handle.net/10220/7731

  21. Lou X et al (2009) Micromagnetic selection of aptamers in microfluidic channels. Proc Natl Acad Sci 106(9):2989–2994. https://doi.org/10.1073/pnas.0813135106

  22. McCloskey KE et al (2003) Magnetophoretic cell sorting is a function of antibody binding capacity. Biotechnol Progress 19(3):899–907. http://doi.wiley.com/10.1021/bp020285e

  23. McCloskey KE, Chalmers JJ, Zborowski M (2003) Magnetic cell separation: characterization of magnetophoretic mobility. Anal Chem 75(24):6868–6874. http://www.ncbi.nlm.nih.gov/pubmed/14670047

  24. Pamme N, Manz A (2004) On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates. Anal Chem 76(24):7250–7256. http://pubs.acs.org/doi/abs/10.1021/ac049183o

  25. Ramadan Q, Poenar DP, Yu C (2009) Customized trapping of magnetic particles. Microfluid Nanofluidics 6(1):53–62. http://link.springer.com/10.1007/s10404-008-0296-2

  26. Vojtíšek M et al (2012) Microfluidic devices in superconducting magnets: on-chip free-flow diamagnetophoresis of polymer particles and bubbles. Microfluid Nanofluidics 13(4):625–635. http://link.springer.com/10.1007/s10404-012-0979-6

  27. Ying TY, Yiacoumi S, Tsouris C (2000) High-gradient magnetically seeded filtration. Chem Eng Sci 55(6):1101–1113

  28. Zhang J et al (2014) Particle inertial focusing and its mechanism in a serpentine microchannel. Microfluid Nanofluidics 17(2):305–316. Available at: http://link.springer.com/10.1007/s10404-013-1306-6

  29. Zhang J et al (2016) A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles. Lab Chip 16:3947–3956. https://doi.org/10.1039/C6LC01007E

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Acknowledgements

The authors are grateful for the help provided by Dr. Bobby Mathew in the lithographic mold fabrication at Khalifa University labs. The soft lithography and experiments were conducted using UAEU facilities.

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Correspondence to Saud A. Khashan.

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Khashan, S.A., Dagher, S. & Alazzam, A. Microfluidic multi-target sorting by magnetic repulsion. Microfluid Nanofluid 22, 64 (2018) doi:10.1007/s10404-018-2083-z

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