A simple yet effective dynamic bead-based microarray is necessary for multiplexed high-throughput screening applications in the fields of biology and chemistry. This paper introduces a microfluidic-based dynamic microbead array system using pneumatically driven elastomeric valves integrated with a microchannel in a single polydimethylsiloxane (PDMS) layer that performs the following functions: single-microbead arraying with loading and trapping efficiencies of 100 %, sequential microbead release for selective retrieval of microbeads of interest, and rapid microarray resettability (<1 s). The key feature is the utilization of an elastomeric membrane as a valve for trapping and releasing single microbeads; this membrane is deformable depending on the applied pneumatic pressure, thereby simply providing a dual trap-and-release function. We propose an effective single-microbead-trapping mechanism based on a dynamic flow-change network and a mathematical model as the design criterion of a trapping site. A sequential microbead release technique via a multistep “release-retrap-and-repeat” method was developed for the selective retrieval of trapped microbeads with a simple configuration consisting of a single PDMS layer and a simple macro-to-micro connection. The proposed dynamic microbead array could be a powerful tool for high-throughput multiplex bead-based drug screening or disease diagnosis.
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Carlo DD, Wu LY, Lee LP (2006) Dynamic single cell culture array. Lab Chip 6:1445–1449
Choi JW, Ahn CH, Bhansali S, Henderson HT (2000) A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems. Sens Actuators B 68:34–39
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem 70:4974–4984
Frimat JP, Becker M, Chiang YY, Marggraf U, Janasek D, Hengstler JG, Franzke J, West J (2011) A microfluidic array with cellular valving for single cell co-culture. Lab Chip 11:231–237
Fu Z, Shao G, Wang J, Lu D, Wang W, Lin Y (2011) Microfabricated renewable beads-trapping/releasing flow cell for rapid antigen-antibody reaction in chemiluminescent immunoassay. Anal Chem 83:2685–2690
Fuard D, Chevolleau TT, Decossas S, Tracqui P, Schiavone P (2008) Optimization of poly-di-methyl-siloxane (PDMS) substrates for studying cellular adhesion and motility. Microelectron Eng 85:1289–1293
Iwai K and Takeuchi S (2009) A dynamic microarray with pneumatic valves for selective trapping and releasing of microbeads. Proceedings of IEEE 22nd International Conference on Micro Electro Mechanical Systems, Sorrento
Iwai K, Tan WH, Ishihara H, Takeuchi S (2011) A resettable dynamic microarray device. Biomed Microdevices 13:1089–1094
Jin HJ, Cho YH, Gu JM, Kim J, Oh YS (2011) A multicellular spheroid formation and extraction chip using removable cell trapping barriers. Lab Chip 11:115–119
Kim H, Lee S, Kim J (2012) Hydrodynamic trap-and-release of single particles using dual-function elastomeric valves: design, fabrication, and characterization. Microfluid Nanofluidcs 13:835–844
Lee GB, Chang CC, Huang SB, Yang RJ (2006) The hydrodynamic focusing effect inside rectangular microchannels. J Micromech Microeng 16:1024–1032
Malainou A, Petrou PS, Kakabakos SE, Gogolides E, Tserepi A (2012) Creating highly dense and uniform protein and DNA microarrays through photolithography and plasma modification of glass substrates. Biosens Bioelectron 34:273–281
Nolan JP, Mandy F (2006) Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems. Cytometry A 69A:318–325
Robinson WH et al (2002) Autoantigen microarrays for multiplex characterization of autoantibody responses. Nat Med 8:295–301
Shao G, Cai Z, Wang J, Wang W and Lin Y (2011) A pneumatic actuated microfluidic beads-trapping device. Proceedings of SPIE7929: Microfluidics, BioMEMS, and Medical Microsystems IX (SPIE 2011), San Francisco, California
Skelley AM, Kirak O, Suh H, Jaenisch R, Voldman J (2009) Microfluidic control of cell pairing and fusion. Nat Methods 6:147–152
Sochol RD, Iwai K, Higa AT, Lo JC, Zhou E, Lo L, Luong C, Dueck M, Li S, Lee LP and Lin L (2010) A resettable high-density microfluidic cell trapping system. Proceedings of 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), Groningen
Sochol RD, Dueck ME, Li S, Lee LP, Lin L (2012) Hydrodynamic resettability for a microfluidic particulate-based arraying system. Lab Chip 12:5051–5056
Steinert CP, Kalkandjiev K, Zengerle R (2009) TopSpot® Vario: a novel microarrayer system for highly flexible and highly parallel picoliter dispensing. Biomed Microdevices 11:755–761
Tan WH, Takeuchi S (2007) A trap-and-release integrated microfluidic system for dynamic microarray applications. Proc Natl Acad Sci 104:1146–1151
Tan WH, Takeuchi S (2008) Dynamic microarray system with gentle retrieval mechanism for cell-encapsulating hydrogel beads. Lab Chip 8:259–266
Tonooka T, Teshima T, Takeuchi S (2012) Clustering triple microbeads in a dynamic microarray for timing-controllable bead-based reactions. Microfluid Nanofluidcs. doi:10.1007/s10404-012-1111-7
Verpoorte E (2003) Beads and chips: new recipes for analysis. Lab Chip 3:60N–68N
Voldman J, Gray ML, Toner M, Schmidt MA (2002) A microfabrication-based dynamic array cytometer. Anal Chem 74:3984–3990
Wang Z, Zhe J (2011) Recent advances in particle and droplet manipulation for lab-on-a-chip devices based on surface acoustic waves. Lab Chip 11:1280–1285
Yamada M, Kano K, Tsuda Y, Kobayashi J, Yamato M, Seki M, Okano T (2007) Microfluidic devices for size-dependent separation of liver cells. Biomed Microdevices 9:637–645
Zhu Z, Frey O, Ottoz DS, Rudolf F, Hierlemann A (2012) Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells. Lab Chip 12:906–915
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MEST) (No. 2011-0030075) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1A2006305). We would like to express our appreciation to Prof. Jehyun Baek and Prof. Dongsung Kim, POSTECH for their support on our experimental setup to perform this work.
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Kim, H., Kim, J. A microfluidic-based dynamic microarray system with single-layer pneumatic valves for immobilization and selective retrieval of single microbeads. Microfluid Nanofluid 16, 623–633 (2014) doi:10.1007/s10404-013-1267-9
- Single-microbead array
- Single-layer pneumatic valve
- Sequential release
- Device resettability