Microfluidics and Nanofluidics

, Volume 15, Issue 6, pp 839–845 | Cite as

Spindle-shaped microfluidic chamber with uniform perfusion flows

  • Hong-Yin Wang
  • Fei-Peng Yang
  • Yan-Qi Wu
  • You-Zhi Xu
  • Huan-Huan Shi
  • Jian-Xin Liu
  • Zheng-Chun LiuEmail author
Research Paper


The reaction chamber is important due to its wide applications. Based on the idea of the bionics, a novel spindle-shaped chamber (S-chamber) for microfluidics was designed to provide uniform flow and eliminate stagnant corners for microchannels. The computational fluid dynamics simulation results demonstrate that these S-chambers have a better performance compared to the conventional diamond-shaped chambers. An S-chamber with the optimized shape, which consists of a rectangle reaction region interfaced to the inlet/outlet channel through an expansion region with smooth arc edges, was fabricated by micromolding of polydimethylsiloxane. This S-chamber was fixed into a microreactor and mounted horizontally on a synthesizer for biochemical reactions. Solvent perfusion experiments and synthetic optimization experiments for in situ synthesis of peptide nucleic acids microarray were performed. The experimental results indicate that the newly designed and fabricated S-chamber provides excellent homogeneous perfusion flows. This type of S-chamber is designed for the most convenient fixation in the horizontal direction, without the need to consider the complicated effect caused by other housing directions. It has a wide application for cell culturing, microarray synthesis, gene hybridization, and many other microfluidic system-based techniques requiring uniform flow conditions.


PDMS Reaction Chamber Peptide Nucleic Acid Reaction Region Expansion Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are grateful for kind comments and revision provided by Prof. Zhan Chen of The University of Michigan. This study was supported financially by the National Natural Science Foundation of China (Grant No. 60801019).

Supplementary material

10404_2013_1195_MOESM1_ESM.doc (430 kb)
Supplementary material 1 (DOC 430 kb)

Supplementary material 2 (AVI 5968 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hong-Yin Wang
    • 1
  • Fei-Peng Yang
    • 1
  • Yan-Qi Wu
    • 1
  • You-Zhi Xu
    • 2
  • Huan-Huan Shi
    • 1
  • Jian-Xin Liu
    • 1
  • Zheng-Chun Liu
    • 1
    Email author
  1. 1.Institute of Biomedical Engineering, School of Geosciences and Info-PhysicsCentral South UniversityChangshaChina
  2. 2.National Engineering Laboratory of Rice and By-product Deep Processing, College of Food Science and EngineeringCentral South University of Forestry and TechnologyChangshaChina

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