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Microfluidics and Nanofluidics

, Volume 15, Issue 6, pp 839–845 | Cite as

Spindle-shaped microfluidic chamber with uniform perfusion flows

  • Hong-Yin Wang
  • Fei-Peng Yang
  • Yan-Qi Wu
  • You-Zhi Xu
  • Huan-Huan Shi
  • Jian-Xin Liu
  • Zheng-Chun LiuEmail author
Research Paper

Abstract

The reaction chamber is important due to its wide applications. Based on the idea of the bionics, a novel spindle-shaped chamber (S-chamber) for microfluidics was designed to provide uniform flow and eliminate stagnant corners for microchannels. The computational fluid dynamics simulation results demonstrate that these S-chambers have a better performance compared to the conventional diamond-shaped chambers. An S-chamber with the optimized shape, which consists of a rectangle reaction region interfaced to the inlet/outlet channel through an expansion region with smooth arc edges, was fabricated by micromolding of polydimethylsiloxane. This S-chamber was fixed into a microreactor and mounted horizontally on a synthesizer for biochemical reactions. Solvent perfusion experiments and synthetic optimization experiments for in situ synthesis of peptide nucleic acids microarray were performed. The experimental results indicate that the newly designed and fabricated S-chamber provides excellent homogeneous perfusion flows. This type of S-chamber is designed for the most convenient fixation in the horizontal direction, without the need to consider the complicated effect caused by other housing directions. It has a wide application for cell culturing, microarray synthesis, gene hybridization, and many other microfluidic system-based techniques requiring uniform flow conditions.

Keywords

PDMS Reaction Chamber Peptide Nucleic Acid Reaction Region Expansion Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful for kind comments and revision provided by Prof. Zhan Chen of The University of Michigan. This study was supported financially by the National Natural Science Foundation of China (Grant No. 60801019).

Supplementary material

10404_2013_1195_MOESM1_ESM.doc (430 kb)
Supplementary material 1 (DOC 430 kb)

Supplementary material 2 (AVI 5968 kb)

References

  1. Adey NB, Lei M et al (2002) Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-mu m-thick chamber. Anal Chem 74(24):6413–6417CrossRefGoogle Scholar
  2. Agbavwe C, Kim C et al (2011) “Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays. J Nanobiotechnol 9:e99CrossRefGoogle Scholar
  3. Barbulovic-Nad I, Lucente M et al (2006) Bio-microarray fabrication techniques—a review. Crit Rev Biotechnol 26(4):237–259CrossRefGoogle Scholar
  4. Blob RW, Bridges WC et al (2008) Morphological selection in an extreme flow environment: body shape and waterfall-climbing success in the Hawaiian stream fish Sicyopterus stimpsoni. Integr Comp Biol 48(6):734–749CrossRefGoogle Scholar
  5. Cho S, Kang DK et al (2011) Recent advances in microfluidic technologies for biochemistry and molecular biology. BMB Reports 44(11):705–712CrossRefGoogle Scholar
  6. Heller MJ (2002) DNA microarray technology: devices, systems, and applications. Annu Rev Biomed Eng 4:129–153CrossRefGoogle Scholar
  7. Hung PJ, Lee PJ et al (2005) A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array. Lab Chip 5(1):44–48CrossRefGoogle Scholar
  8. Kim MS, Kim T et al (2010) “Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform.” Plos One 5(5):e10441Google Scholar
  9. Liu ZC, Shin DS et al (2007) Light-directed synthesis of peptide nucleic acids (PNAs) chips. Biosens Bioelectron 22(12):2891–2897CrossRefGoogle Scholar
  10. Nindl I, Toegl A et al (2004) High sensitivity and reproducibility of immunohistochemistry with microagitation. Arch Dermatol Res 296(6):278–281CrossRefGoogle Scholar
  11. Okkels F, Dufva M et al (2011) “Optimal homogenization of perfusion flows in microfluidic bio-reactors: a numerical study.” Plos One 6(1):e14574Google Scholar
  12. Petronis S, Stangegaard M et al (2006) Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion. Biotechniques 40(3):368–376CrossRefGoogle Scholar
  13. Sabourin D, Snakenborg D et al (2010) Interconnection blocks with minimal dead volumes permitting planar interconnection to thin microfluidic devices. Microfluid Nanofluid 9(1):87–93CrossRefGoogle Scholar
  14. Soe MJ, Okkels F et al (2011) HistoFlex-a microfluidic device providing uniform flow conditions enabling highly sensitive, reproducible and quantitative in situ hybridizations. Lab Chip 11(22):3896–3907CrossRefGoogle Scholar
  15. Vinuselvi P, Park S et al (2011) Microfluidic technologies for synthetic biology. Int J Mol Sci 12(6):3576–3593CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hong-Yin Wang
    • 1
  • Fei-Peng Yang
    • 1
  • Yan-Qi Wu
    • 1
  • You-Zhi Xu
    • 2
  • Huan-Huan Shi
    • 1
  • Jian-Xin Liu
    • 1
  • Zheng-Chun Liu
    • 1
    Email author
  1. 1.Institute of Biomedical Engineering, School of Geosciences and Info-PhysicsCentral South UniversityChangshaChina
  2. 2.National Engineering Laboratory of Rice and By-product Deep Processing, College of Food Science and EngineeringCentral South University of Forestry and TechnologyChangshaChina

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