A cyclic particle extractor based on particle diffusion is presented. The extraction realized by the device features simplicity, programmability, and low cost. Although conventional particle separation based on diffusion can be spontaneously realized without any active inputs, the extraction efficiency decreases as the size difference between particles decreases or if the diffusion length is insufficient. In this article, a primary extraction procedure including four operational steps is proposed to facilitate the process. By simply repeating the procedure, the separation scheme is additive, and increased efficiency is observed with each additional cycle. A mixture of 0.5- and 3-μm polystyrene particles was separated in up to 10 extraction cycles. Using a 2.5-Hz phase frequency, the average flow velocity was 2.5 mm/s. An unequal volume ratio of the sample stream to extraction stream (45:55) created a barrier region to help minimize unwanted (large) particles from entering the extraction stream. The initial concentration of the extracted small particles was 7.5% after 2 cycles, but jumped up to 38% after 10 cycles.
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Atebcia J, Beebe DJ (2006) Steady flow generation in microcirculatory systems. Lab Chip 6:567–574
Bhagat ASS, Kuntaegowdanahalli SS, Papautsky I (2008) Continuous particle separation in spiral microchannels using dean flows and differential migration. Lab Chip 8:1906–1914
Bowden SA, Monaghan PB, Wilson R, Parnell J, Cooper JM (2006) The liquid-liquid diffusive extraction of hydrocarbons from a North Sea oil using a microfluidic format. Lab Chip 6:740–743
Brody JP, Yager P (1997) Diffusion-based extraction in a microfabricated device. Sens Actuators A 58:13–18
Brody JP, Osborne TD, Forster FK, Yager P (1996) A planar microfabricated fluid filter. Sens Actuators A 54:704–708
Carbajal-Tinoco MD, Lopez-Fernandez R, Arauz-Lara L (2007) Assymetry in colloidal diffusion near a rigid wall. Phys Rev Lett 99:138303
Cho BS, Schuster TG, Zhu X, Chang D, Smith GD, Takayama S (2003) Passively driven integrated microfluidic system for separation of motile sperm. Anal Chem 75(7):1671–1675
Christensen AM, Chang-Yen DA, Gale BK (2005) Characterization of interconnects used in PDMS microfluidic systems. J Micromech Microeng 15:928–934
Chuang HS, Wereley ST (2009a) Design, fabrication and characterization of a conducting PDMS for microheaters and temperature sensors. J Micromech Microeng 19:045010
Chuang HS, Wereley ST (2009b) Rapid patterning of slurry-like elastomer composites using a laser-cut tape. J Micromech Microeng 19:097001
Chuang HS, Amin AM, Wereley ST, Thottethodi M, Vijaykumar TN, Jacobson SC (2008) Polydimethylsiloxane (PDMS) peristaltic pump characterization for programmable lab-on-a-chip applications. In: Proceedings of the 12th international conference on miniaturized systems for chemistry and life Sciences (μTAS 2008), San Diego, CA
Frej NA, Prieve DC (1993) Hindered diffusion of a single sphere very near a wall in a nonuniform force field. J Chem Phys 98:7552–7564
Grafton MM, Geheb B, Jang JH, Chuang HS, Rajdev P, Reece LM, Irazoqui PP, Wereley ST, Jung B, Leary JF (2009) Microfabrication of a two-stage BioMEMS microfluidic cell sorter. Proc SPIE 7207:72070A
Grover WH, Skelley AM, Liu CN, Lagally ET, Mathies RA (2003) Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices. Sens Actuators B 89:315–323
Haubert K, Drierb T, Beebe D (2006) PDMS bonding by means of a portable, low-cost corona system. Lab Chip 6:1548–1549
Holl MR, Galambos P, Forster FK, Brody JP, Afromowitz M, Yager P (1996) Optimal design of a microfabricated diffusion-based extraction device. In: ASME on micro electromechanical systems, pp 183–189
Huang P, Breuer KS (2007) Direct measurement of anisotropic near-wall hindered diffusion using total internal reflection velocimety. Phys Rev Lett E76:046307
Kapishnikov S, Kantsler V, Steinberg V (2006) Continuous particle size separation and size sorting using ultrasound in a microchannel. J Stat Mech Theory Exp:P01012
Kihm KD, Banerjee A, Choi CK, Takagi T (2004) Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM). Exp Fluids 37:811–824
Kralj JG, Lis MTW, Schmidt MA, Jensen KF (2006) Continuous dielectrophoretic size-based particle sorting. Anal Chem 78:5019–5025
Kumar A, Gorti VM, Shang H, Lee GU, Yip NK, Wereley ST (2008) Optical diffusometry techniques and applications in biological agent detection. J Fluids Eng 130(11):111401
Lee GB, Lin CH, Chang SC (2005) Micromachine-based multi-channel flow cytometers for cell/particle counting and sorting. J Micromech Microeng 15:447–454
Lillehoj P, Li N, Tsutsui H, Ho CH (2008) A compact microfluidic continuous flow separator for particle and cell sorting. In: MEMS 2008, IEEE, Tucson, AZ
Lin CH, Lee CY, Tsai CH, Fu LM (2009) Novel continuous particle sorting in microfluidic chip utilizing cascaded squeeze effect. Microfluid Nanofluid 7(4):499–508
Marc PJ, Sims CE, Allbritton NL (2007) Coaxial flow system for chemical cytometry. Anal Chem 79:9054–9059
Niu X, Zhang M, Peng S, Wen W, Sheng P (2007) Real-time detection, control, and sorting of microfluidic droplets. Biomicrofluidics 1:044101
Sadr R, Li H, Yoda M (2005) Impact of hindered Brownian diffusion on the accuracy of particle-image velocimetry using evanescent-wave illumination. Exp Fluids 38:90–98
Williams SJ, Kumar A, Wereley ST (2008) Electrokinetic patterning of colloidal particles with optical landscapes. Lab Chip 8(11):1879–1882
Yang SY, Hsiung SK, Hung YC, Chang CM, Liao TL, Lee GB (2006) A cell counting/sorting system incorporated with a microfabricated flow cytometer chip. Meas Sci Technol 17:2001–2009
The authors thank National Science Foundation grant NSF CCF-0726821 and NSF CCF-0726694 for support of this work. The authors also acknowledge the technical assistances from Purdue Birck Nanotechnology Center.
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Chuang, H., Jacobson, S.C. & Wereley, S.T. A diffusion-based cyclic particle extractor. Microfluid Nanofluid 9, 743–753 (2010) doi:10.1007/s10404-010-0589-0
- Particle sorting
- H filter