Journal of Medical Ultrasonics

, Volume 46, Issue 1, pp 35–43 | Cite as

Ultrasonic measurement of sound velocity fluctuations in biological tissue due to ultrasonic heating and estimation of thermo-physical properties

  • Yukako Tsujimoto
  • Mai Morimoto
  • Naotaka Nitta
  • Iwaki AkiyamaEmail author
Original Article



Tissue characterization in terms of the differences in thermo-physical properties of biological tissues was investigated in this study. The objective was to measure the ratio of variation in sound velocity due to ultrasonic heating and to derive the relational expression between the ratio and thermo-physical properties.


The ratio of sound velocity variation before and after the temperature rise of tissue samples exposed to ultrasound was measured by ultrasonic pulse echo method. The thermo-physical properties were estimated for a tissue-mimicking material and porcine muscle and fat tissues due to theoretical expression. The transducer for heating had a resonance frequency of 3.2 MHz, and the transducer for measurement of sound velocity variation had a resonance frequency of 5.2 MHz.


In the phantom study, the measured values of the temperature rise agreed with the values calculated by a finite element method (FEM). The estimated values of the temperature rise from the sound velocity variations of muscle and fat tissues were 0.36 °C and 1.1 °C, respectively. Also, the estimated values of thermo-physical properties agreed with the reference values within an error of 10%.


The thermo-physical properties of the porcine tissues were measured by sound velocity variation due to ultrasonic heating within the safety regulations.


Tissue characterization Thermo-physical properties Sound velocity variation Temperature rise 



This study is supported by MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2013-2017.

Compliance with ethical standards

Conflict of interest

Yukako Tsujimoto, Mai Morimoto, Naotaka Nitta, and Iwaki Akiyama declare that they have no conflicts of interest.

Human and animal rights

This article does not contain any studies with human or animal subjects performed by any of the authors.


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Copyright information

© The Japan Society of Ultrasonics in Medicine 2018

Authors and Affiliations

  • Yukako Tsujimoto
    • 1
  • Mai Morimoto
    • 1
  • Naotaka Nitta
    • 2
  • Iwaki Akiyama
    • 1
    Email author
  1. 1.Medical Ultrasound Research CenterDoshisha UniversityKyotanabeJapan
  2. 2.National Institute of Advanced Industrial Science and TechnologyTsukubaJapan

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