Impact of tumor-infiltrating LAMP-3 dendritic cells on the prognosis of esophageal squamous cell carcinoma
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Dendritic cells (DCs) are the most potent antigen-presenting cells to induce cytotoxic T lymphocytes in the tumor environment. After acquiring antigens, DCs undergo maturation and their expression of MHC and co-stimulation molecules are enhanced, along with lysosome-associated membrane glycoprotein 3 (LAMP-3), which is a specific marker of mature DCs. In general, mature DCs are usually considered to be immunostimulatory in the cancer microenvironment. In addition, it is known that tumor-infiltrating lymphocytes (TILs) are associated with a good prognosis in esophageal squamous cell carcinoma (ESCC). However, few studies have targeted the interaction between DCs and TILs in the local immunity of ESCC. We investigated the localization of mature DCs within ESCC tissue and their relationship to TILs as well as the clinical outcome.
We evaluated 80 ESCC patients who underwent surgical treatment without preoperative treatment, using immunohistochemistry with LAMP-3 and CD8.
The results showed that LAMP-3 DCs were predominantly observed in the peritumoral area. Intratumoral CD8 T cells were found to be associated with a favorable prognosis, and the number of infiltrating LAMP-3 DCs was correlated with the number of intratumoral CD8 T cells.
At the local tumor site, mature LAMP-3 DCs might be associated with increasing tumor infiltrating CD8 T cells.
KeywordsEsophageal Dendritic cell LAMP-3
Compliance with ethical standards
Our investigation was carried out according to the Declaration of Helsinki. All experimental procedures after 2013 were approved as no. 3138 by the Osaka City University ethics committee, and all patients had provided informed consent for collection and analysis of the specimens. On the other hand, because the other experimental procedures up to 2013 were only all-inclusive consent, we are showing opt-out form as an observational study.
Conflict of interest
All authors declare that there is no conflict of interest in the present study.
- 7.Lill PH, Fortner GW. Identification and cytotoxic reactivity of inflammatory cells recovered from progressing or regressing syngeneic UV-induced murine tumors. J Immunol. 1978;121:1854–60.Google Scholar