, Volume 16, Issue 4, pp 333–344 | Cite as

Impact of tumor-infiltrating LAMP-3 dendritic cells on the prognosis of esophageal squamous cell carcinoma

  • Junya Nishimura
  • Hiroaki TanakaEmail author
  • Yoshihito Yamakoshi
  • Soichiro Hiramatsu
  • Tatsuro Tamura
  • Takahiro Toyokawa
  • Kazuya Muguruma
  • Kiyoshi Maeda
  • Kosei Hirakawa
  • Masaichi Ohira
Original Article



Dendritic cells (DCs) are the most potent antigen-presenting cells to induce cytotoxic T lymphocytes in the tumor environment. After acquiring antigens, DCs undergo maturation and their expression of MHC and co-stimulation molecules are enhanced, along with lysosome-associated membrane glycoprotein 3 (LAMP-3), which is a specific marker of mature DCs. In general, mature DCs are usually considered to be immunostimulatory in the cancer microenvironment. In addition, it is known that tumor-infiltrating lymphocytes (TILs) are associated with a good prognosis in esophageal squamous cell carcinoma (ESCC). However, few studies have targeted the interaction between DCs and TILs in the local immunity of ESCC. We investigated the localization of mature DCs within ESCC tissue and their relationship to TILs as well as the clinical outcome.


We evaluated 80 ESCC patients who underwent surgical treatment without preoperative treatment, using immunohistochemistry with LAMP-3 and CD8.


The results showed that LAMP-3 DCs were predominantly observed in the peritumoral area. Intratumoral CD8 T cells were found to be associated with a favorable prognosis, and the number of infiltrating LAMP-3 DCs was correlated with the number of intratumoral CD8 T cells.


At the local tumor site, mature LAMP-3 DCs might be associated with increasing tumor infiltrating CD8 T cells.


Esophageal Dendritic cell LAMP-3 


Compliance with ethical standards

Ethical statement

Our investigation was carried out according to the Declaration of Helsinki. All experimental procedures after 2013 were approved as no. 3138 by the Osaka City University ethics committee, and all patients had provided informed consent for collection and analysis of the specimens. On the other hand, because the other experimental procedures up to 2013 were only all-inclusive consent, we are showing opt-out form as an observational study.

Conflict of interest

All authors declare that there is no conflict of interest in the present study.


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Copyright information

© The Japan Esophageal Society and Springer 2019

Authors and Affiliations

  • Junya Nishimura
    • 1
  • Hiroaki Tanaka
    • 1
    Email author
  • Yoshihito Yamakoshi
    • 1
  • Soichiro Hiramatsu
    • 1
  • Tatsuro Tamura
    • 1
  • Takahiro Toyokawa
    • 1
  • Kazuya Muguruma
    • 1
  • Kiyoshi Maeda
    • 1
  • Kosei Hirakawa
    • 1
  • Masaichi Ohira
    • 1
  1. 1.Department of Surgical OncologyOsaka City University Graduate School of MedicineOsakaJapan

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