Comparison of esophageal submucosal glands in experimental models for esophagus tissue engineering applications
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Esophagus tissue engineering holds promises to overcome the limitations of the presently employed esophageal replacement procedures. This study investigated 5 animal models for esophageal submucosal glands (ESMG) to identify models appropriate for regenerative medicine applications. Furthermore, this study aimed to measure geometric parameters of ESMG that could be utilized for fabrication of ESMG-specific scaffolds for esophagus tissue engineering applications.
Ovine, avian, bovine, murine, and porcine esophagus were investigated using Hematoxylin–Eosin (HE), Periodic Acid Schiff (PAS), and Alcian Blue (AB), with AB applied in 3 pH levels (0.2, 1.0, and 2.5) to detect sulphated mucous. Celleye® (version F) was employed to gain parametric data on ESMGs (size, perimeter, distance to lumen, and acini concentration) necessary for scaffold fabrication.
Murine, bovine, and ovine esophagus were devoid of ESMG. Avian esophagus demonstrated sulphated acid mucous producing ESMGs with a holocrine secretion pattern, whereas sulphated acid and neutral mucous producing ESMGs with a merocrine secretion pattern were observed in porcine esophagus. Distance of ESMGs to lumen ranged from 127–340 μm (avian) to 916–983 μm (porcine). ESMGs comprised 35% (avian) to 45% (porcine) area of the submucosa. ESMG had an area of 125000 μm2 (avian) to 580000 μm2 (porcine).
Avian and porcine esophagus possesses ESMGs. However, porcine esophagus correlates with data available on human ESMGs. Geometric and parametric data obtained from ESMG are valuable for the fabrication of ESMG-specific scaffolds for esophagus tissue engineering using the hybrid construct approach.
KeywordsEsophagus Submucosal glands Scaffolds Tissue engineering Animal models
This research was funded by a European Union Grant within the 6th Framework Program (EuroSTEC; LSHC-CT-2006-037409).
Compliance with ethical standards
This work conforms to the guidelines set forth in the Helsinki Declaration of 1975, as revised in 2000 (5), concerning Human and Animal Rights, and that all institutional and national guidelines for the care and use of laboratory animals were followed.
Conflict of interest
Both authors declare no conflict of interest.
- 1.Clark DC. Esophageal atresia and tracheoesophageal fistula. Am Fam Phycisian. 1999;59:910–6.Google Scholar
- 19.Kofler K, Ainoedhofer H, Tausendschön J, Höllwarth ME, Saxena AK. Esophageal smooth muscle cells dedifferentiate with loss of a-smooth muscle actin expression after 8 weeks of explant expansion in vitro culture: implications on esophagus tissue engineering. Eur Surg. 2011;43:168–73.CrossRefGoogle Scholar
- 20.Kofler K, Ainoedhofer H, Höllwarth ME, Saxena AK. Fluorescence-activated cell sorting of PCK-26 antigen-positive cells enables selection of ovine esophageal epithelial cells with improved viability on scaffolds for esophagus tissue engineering. Pediatr Surg Int. 2010;26:97–104.CrossRefPubMedGoogle Scholar
- 30.Deinhofer MG (2008) Anatomie von Schaf und Ziege. Österreichischer Bundesverband für Schafe und Ziegen, Vienna 1–20Google Scholar