First assessment of MHC diversity in wild Scottish red deer populations
Control and mitigation of disease in wild ungulate populations are one of the major challenges in wildlife management. Despite the importance of the major histocompatibility complex (MHC) genes for immune response, assessment of diversity on these genes is still rare for European deer populations. Here, we conducted the first assessment of variation at the second exon of the MHC DRB in wild populations of Scottish highland red deer, the largest continuous population of red deer in Europe. Allelic diversity at these loci was high, with 25 alleles identified. Selection analyses indicated c. 22% of amino acids encoded under episodic positive selection. Patterns of MHC allelic distribution were not congruent with neutral population genetic structure (estimated with 16 nuclear microsatellite markers) in the study area, the latter showing a marked differentiation between populations located at either side of the Great Glen. This study represents a first step towards building an immunogenetic map of red deer populations across Scotland to aid future management strategies for this ecologically and economically important species.
KeywordsCervus elaphus Immunogenetics Major histocompatibility complex Population structure Red deer Wildlife management
Deer stalkers and deer managers of the estates of Tarlogie, Strathconon, Inshriach and Abernethy are greatly thanked for the collection of samples. A. Jones, K. Russell, S. Joinson and J. Hennessy are thanked for assistance with microsatellite genotyping and S. Requena (CSIC) for map reproduction. Cambridge Conservation Forum and the Cambridge Conservation Initiative are thanked for allowing Sílvia Pérez-Espona to use their office space at the David Attenborough Building while preparing this manuscript.
This study was funded by the British Deer Society and samples were obtained from a project funded through Rural Affairs Food and Environment Strategic Research-Scottish Government.
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