A new baiting scheme and simple method of rhodamine B detection could improve biomarking of small mammals
- 20 Downloads
The success of group biomarking with rhodamine B (RB) is determined by two key stages—baiting and mark detection. This work has two objectives: (1) to examine the suitability of a new bait type and the proposed experimental procedures for marking small mammals with RB and (2) to evaluate the effectiveness of the new method of RB detection in field studies of small mammals. Uniquely designed bait with RB was placed in nine rectangular plots in forest stands. The experiments differed in the area of the marking plots, application rate of the bait, and the time interval before the start of animal trapping. Small mammals were captured and evaluated in a dark room for the presence of RB markings. A green laser with a diffuser was used as the illuminator to excite fluorescence, and orange glass was used for separation of the emission spectrum. The new method for the detection of RB was shown to be simple and reliable. The bait used in this study was proven to be effective and is potentially suitable for most species of small mammals, especially rodents, under certain conditions.
KeywordsBiomarker Fluorescence Mark detection Rhodamine B Small mammals Group marking
This study was performed within the frameworks of state contract with the Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences.
Compliance with ethical standards
This research follows national guidelines for the care and use of animals. I did not use any unusual protocols that could contradict the generally accepted standards of animal care.
Conflict of interest
The author declares that he has no conflict of interest.
- Fichet-Calvet E (1999) Persistence of a systemic labelling in fur and guard hairs by ingestion of rhodamine B in Myocastor coypus (Rodentia). Mammalia 63:241–244Google Scholar
- Fisher P (1999) Review of using Rhodamine B as a marker for wildlife studies. Wildl Soc Bull 27:318–329Google Scholar
- Grigorkina EB, Olenev GV (2013) Migration of rodents in the eastern Urals radioactive trace zone (radiobiological aspect). Radiat Biol Radioecol 53:76–83 (in Russian)Google Scholar
- Hanski I, Gaggiotti OE (2004) Ecology, genetics and evolution of metapopulations. Elsevier Academic Press, AmsterdamGoogle Scholar
- Klevezal GA, Mina MV (1980) Tetracycline method of group marking for rodents and prospect of its utilization in ecological studies. Zoologicheskii Zhurnal 59:936–941 (in Russian)Google Scholar
- Linn IJ (1978) Radioactive techniques for small mammal marking. In: Stonehouse B (ed) Animal marking. Recognition Marking of Animals in Research. The Macmillan press LTD, London, pp 177–191Google Scholar
- Turchin P (1998) Quantitative analysis of movement: measuring and modeling population redistribution in animals and plants. Sinauer Associates, SunderlandGoogle Scholar