Micropropagation of Three Commercial Cultivars of Hazelnut (Corylus avellana L.)

  • Neda Mardani
  • Ali KhadiviEmail author
  • Ali Vatanpour-Azghandi
Original Article


The traditional methods of propagation of hazelnuts are time-consuming and not satisfactory at the commercial level. Therefore, the goal of current investigation was to optimize micropropagation of three commercial cultivars of hazelnut including ‘Gercheh’, ‘Gerdooyi’ and ‘Pashmineh’. In the present experiment, the effects of four levels of Benzylaminopurine (BAP; 0.50, 1.00, 2.00 and 4.00 mg L−1) with kinetin (0.25 mg L−1) and without kinetin on the shoot induction and proliferation were investigated. In addition, the effects of six treatments (NRM3 [control], NRM3/2, 0.50 mg L−1 IBA + 0.50 mg L−1 NAA, 0.50 mg L−1 IBA, 1.00 mg L−1 IBA + 1.00 mg L−1 NAA, and 0.50 mg L−1 NAA) on rooting of the studied cultivars were studied. Results showed that the best treatment for shoot proliferation for ‘Gercheh’ was 0.25 mg L−1 kinetin + 0.50 mg L−1 BAP as well as the best treatment for rooting of this cultivar was 1.00 mg L−1 IBA + 1.00 mg L−1 NAA. In ‘Gerdooyi’, the optimum treatment for shoot proliferation was 0.25 mg L−1 kinetin + 4.00 mg L−1 BAP, while the best treatment for its rooting was 0.50 mg L−1 IBA + 0.50 mg L−1 NAA. In ‘Pashmineh’, the best treatment for shoot proliferation was 0.00 mg L−1 kinetin + 0.50 mg L−1 BAP. In addition, the best treatment for rooting of this cultivar was NRM3/2. The results presented herein could be used for micropropagation of hazelnut.


Hazelnut Tissue culture Corylus avellana Shoot proliferation Rooting 

Mikrovermehrung von drei handelsüblichen Haselnusssorten (Corylus avellana L.)


Die traditionellen Methoden der Vermehrung von Haselnüssen sind zeitaufwändig und auf kommerzieller Ebene nicht zufriedenstellend. Ziel der aktuellen Untersuchung war es daher, die Mikrovermehrung von drei kommerziellen Haselnusssorten zu optimieren. Im vorliegenden Experiment wurden die Auswirkungen von vier Benzylaminopurinkonzentrationen (BAP; 0,50, 1,00, 2,00 und 4,00 mg L−1) mit Kinetin (0,25 mg L−1) und ohne Kinetin auf die Sprossinduktion und -proliferation untersucht; ebenso die Auswirkungen von sechs Behandlungen (NRM3 [Kontrolle], NRM3/2, 0,50 mg L−1 IBA + 0,50 mg L−1 NAA, 0,50 mg L−1 IBA, 1,00 mg L−1 IBA + 1,00 mg L−1 NAA und 0,50 mg L−1 NAA) auf die Wurzelbildung der untersuchten Haselnusssorten. Die Ergebnisse zeigten, dass die beste Behandlung für die Sprossproliferation für die Sorte ‘Gercheh’ 0,25 mg L−1 Kinetin + 0,50 mg L−1 BAP war. Die beste Behandlung für diese Sorte bezüglich der Wurzelbildung war 1,00 mg L−1 IBA + 1,00 mg L−1 NAA. Bei der Sorte ‘Gerdooyi’ war die optimale Behandlung für die Sprossproliferation 0,25 mg L−1 Kinetin + 4,00 mg L−1 BAP, während die beste Behandlung für die Wurzelbildung 0,50 mg L−1 IBA + 0,50 mg L−1 NAA war. In der Sorte ‘Pashmineh’ war 0,00 mg L−1 Kinetin + 0,50 mg L−1 BAP die beste Behandlung für die Sprossproliferation. Die beste Behandlung für die Verwurzelung dieser Sorte war NRM3/2. Die hier präsentierten Ergebnisse könnten für die Mikrovermehrung von Haselnüssen verwendet werden.


Haselnuss Gewebekultur Corylus avellana Sprossproliferation Wurzelbildung 


Conflict of interest

N. Mardani, A. Khadivi and A. Vatanpour-Azghandi declare that they have no competing interests.


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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2019

Authors and Affiliations

  • Neda Mardani
    • 1
  • Ali Khadivi
    • 1
    Email author
  • Ali Vatanpour-Azghandi
    • 2
  1. 1.Department of Horticultural Sciences, Faculty of Agriculture and Natural ResourcesArak UniversityArakIran
  2. 2.Agriculture Biotechnology Research Institute of Iran (ABRII)KarajIran

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