Pollen Tube Growth and Embryo Sac Development in ‘Pozna Plava’ Plum Cultivar Related to Fruit Set
- 24 Downloads
A newly released, late ripening plum cultivar ‘Pozna Plava’ sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as well as initial and final fruit set have been studied. In addition to this, the impact made by temperature fluctuations on the interaction between male gametophytes and female sporophytes was also analysed. Growth of the pollen tubes in the style and penetration into the nucellus as well as fruit set were more effective in cross-pollination than in open and self-pollination. A relative delay in ovule development was observed, and most ovules had an embryo sac with eight nuclei. Considering the results of the quantitative parameter study of pollen tube growth in the ovary as well as the results of the stage of ovule development, a conclusion can be made that this cultivar is characterised by an extremely short effective period of pollination.
KeywordsPrunus domestica L. Ovule Embryo sac Pollen tube growth Pollen viability Fruit set
Pollenschlauch-Wachstum und Embryosack-Entwicklung bei der Pflaumensorte ‘Pozna Plava’ im Verhältnis zum Fruchtansatz
SchlüsselwörterPrunus domestica L. Samenanlage Embryosack Pollenschlauch-Wachstum Lebensfähigkeit des Pollens
This work was conducted under Research Project TR-31064: Development and preservation of genetic potential of temperate zone fruits, supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia.
Conflict of interest
M. Đorđević, R. Cerović, S. Radičević, D. Nikolić, N. Milošević, I. Glišić, S. Marić and M. Lukić declare that they have no competing interests.
- Cerović R (1994) Histocitološki aspekti dinamike oplodnje kod višnje (Prunus cerasus L.). University of Belgrade, Belgrade (Dissertation)Google Scholar
- Cerović R, Mićić NŽ (1996) Oprašivanje i oplodnja jabučastih i koštičavih voćaka. J Yugosl Pomol 30:73–98Google Scholar
- Đorđević M, Cerović R, Radičević S, Nikolić D, Marić S, Milošević N, Glišić I (2016) Influence of pollination variant on fruit set in plum (Prunus domestica L.). Acta Hortic 1139:347–352Google Scholar
- Dyś B (1984) Development of embryo sacs and ovules at some stages of florescence. Genet Polonica 25:171–180Google Scholar
- Fischer A (1951) The desing of experiments, 6th edn. Olyver and Boyd, LondonGoogle Scholar
- Furukawa Y, Bukovac MJ (1989) Embryo sac development in sour cherry during the pollination period as related to fruit set. HortScience 24:1005–1008Google Scholar
- Gerlach D (1969) A rapid safranin-crystal violet-light green staining sequence for paraffin sections of plant materials. Stain Technol 44:210–211Google Scholar
- Kho YO, Baër J (1971) Fluorescence microscopy in botanical research. Zeiss Inf 76:54–57Google Scholar
- Lukić M, Pešaković M, Marić S, Glišić I, Milošević N, Radičević S, Leposavić A, Đorđević M et al (2016) Sorte voćaka stvorene u Institutu za voćarstvo, Čačak (1946–2016). Institut za voćarstvo, ČačakGoogle Scholar
- Preil W (1970) Observing the growth of pollen tubes in pistil and ovarian tissue by means of fluorescence microscopy. Zeiss Inf 75:24–25Google Scholar
- Stösser R, Anvari SF (1981) Pollen tubes growth in the pericarp tissue of cherries. Gartenbauwissenschaft 46:154–158Google Scholar
- Thompson MM, Liu LJ (1973) Temperature, fruit set and embryo sac development in Italian prune. J Am Soc Hortic Sci 98:193–197Google Scholar
- Williams RR (1970) Factors affecting pollination in fruit trees. In: Luckwill LC, Cutting CV (eds) Physiology of tree crops. Academic Press, London, New YorkGoogle Scholar