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Low risk of resistance evolution of Spodoptera frugiperda to chlorfenapyr in Brazil

  • Rubens H. Kanno
  • Anderson Bolzan
  • Ingrid S. Kaiser
  • Ewerton C. Lira
  • Fernando S. A. Amaral
  • Aline S. Guidolin
  • Antônio R. B. Nascimento
  • Celso OmotoEmail author
Original Paper
  • 204 Downloads

Abstract

Insecticide resistance is serious problem for Spodoptera frugiperda (Lepidoptera: Noctuidae). Due to intense selection pressure, the evolution of S. frugiperda resistance to major insecticides groups and Bt proteins has already been documented. Therefore, studies to preserve the lifetime of insecticides with unique modes of action such as chlorfenapyr are important in insect resistance management programs. For implementing a proactive resistance management program of S. frugiperda to chlorfenapyr, studies were conducted with field populations collected from major corn-growing regions in Brazil from 2016 to 2018. We aimed to (i) characterize and monitor the susceptibility of field populations, (ii) estimate the frequency of resistance alleles and (iii) evaluate cross-resistance patterns between insecticides and Bt-resistant strains of S. frugiperda to chlorfenapyr. Baseline susceptibility of field population showed a LC50 ranged from 18.3 to 25.1 µg ml−1. The survival rate at the diagnostic concentration ranged from 0 to 8.4%. Using the F2 screen method, the overall estimated resistance allele frequency was 0.0003. No cross-resistance was observed between chlorfenapyr and other insecticides and Bt proteins. Results demonstrated a high susceptibility, low frequency of resistance alleles and lack of cross-resistance. Thus, we concluded there is a low risk of resistance evolution of S. frugiperda to chlorfenapyr in Brazil using the current pest management strategies.

Keywords

Fall armyworm Baseline susceptibility Susceptibility monitoring F2 screen Cross-resistance Insect resistance management 

Notes

Acknowledgements

We thank Coordination for the Improvement of Higher Education Personnel (CAPES)—Finance Code 001 for granting a scholarship to the first author and the National Council for Scientific and Technological Development (CNPq) for the fellowship to CO (Grant #403851/2013-0). We also thank the Brazilian Insecticide Resistance Action Committee (IRAC-BR) (Grant Project 2975-0) for providing partial financial support for this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animals rights

This article does not contain any studies with human participants or animals performed by any of the authors.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture (ESALQ)University of São Paulo (USP)PiracicabaBrazil

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