Comparative expression of two detoxification genes by Callosobruchus maculatus in response to dichlorvos and Lippia adoensis essential oil treatments
- 94 Downloads
The cowpea beetle, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae), is a field-to-store pest, which can cause up to 80% damage of cowpea grains within 3 months of storage. The control approach consisting of application of synthetic pesticides has become challenging following the increased resistance and toxicity to non-target organisms and the environment. Here, we hypothesized that Lippia adoensis essential oil (EO) (plant-based insecticide) can repress cytochrome P450-dependent mono-oxygenase and glutathione-S-transferase (GST) genes and suppress C. maculatus resistance to dichlorvos (O,O-dimethyl-O-2,2-dichlorovinylphosphate or DDVP). The methods consisted of separately exposing C. maculatus adults to cowpea seeds treated with DDVP and L. adoensis EO. Their physiological and molecular responses were monitored for five generations. Adult mortality of DDVP-treated beetles significantly decreased across generations and negatively correlated with the reproduction parameters (increase in fecundity and adult emergence) and seed damage. Similarly, the decrease in adult susceptibility corresponded with the increase in the expression levels of cytochrome P450 and GST genes (overexpression of genes). However, the adult susceptibility to EO treatments remained consistent across generations and correlated with the down-regulation of targeted genes from the third generation (F3). These results support our hypotheses and provide a probable molecular basis of resistance to DDVP and susceptibility to L. adoensis EO in C. maculatus. Therefore, L. adoensis EO represents an alternative insecticide that could be employed to enhance the vulnerability of this pest.
KeywordsCytochrome P450 Glutathione-S-transferase Cowpea beetle Pesticide biotransformation Fitness parameters Detoxification
We thank the Chinese Government for the scholarship (2015120T22) granted to the first author and the National Natural Science Foundation of China (31661143045), Agricultural Public Welfare Industry Research supported by Ministry of Agriculture of People’s Republic of China (201503137) and the Fundamental Research Funds for the Central Universities (2662015PY148) (CYN) for providing funding for this work. Special thanks are due to Professor Kun Yan Zhu (College of Agriculture, Department of Entomology, Kansas State University), Dr Awawing A. Andongma, and Dr Adnan Muhammad Rashid (College of Plant Science and Technology, Huazhong Agricultural University) for their advice and technical assistance.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any study with human or animal participants performed by any of the authors. The study was approved by the Scientific Committee of the Faculty of Science and validated by the Scientific Committee of the University of Ngaoundere (Cameroon) (Decision Number 2015/093) in a full PhD program assigned to the first author.
- Adelani BS, Olusegun OS, Olulakin AG, Adeolu AM (2016) Chemical composition and bioactivity of Lippia adoensis Hochst ex. Walp (Verneneaceae) leaf essential oil against Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae). J Northeast Agric Univ 23:8–14Google Scholar
- Akami M, Niu C, Chakira H, Chen Z, Vandi T, Nukenine EN (2016) Persistence and comparative pesticidal potentials of some constituents of Lippia adoensis (Hochst. ex Walp.) (Lamiales: Verbenaceae) essential oil against three life stages of Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae). Br Biotechnol J 13:1–16. https://doi.org/10.9734/BBJ/2016/26087 CrossRefGoogle Scholar
- Amusa OD, Ogunkanmi AL, Bolarinwa K, Ojobo O (2013) Evaluation of four cowpea lines for bruchid (Callosobruchus maculatus) tolerance. J Nat Sci Res 3:46–52Google Scholar
- Bernays EA, Chapman RF (2007) Host-plant selection by phytophagous insects, vol 2. Springer Science & Business Media, New YorkGoogle Scholar
- Crava CM, Brütting C, Baldwin IT (2016) Transcriptome profiling reveals differential gene expression of detoxification enzymes in a hemimetabolous tobacco pest after feeding on jasmonate-silenced Nicotiana attenuata plants. BMC Genom 17:1005. https://doi.org/10.1186/s12864-016-3348-0 CrossRefGoogle Scholar
- Feyereisen R (2012) Insect CYP genes and P450 enzymes. Insect Biochem Mol Biol 8:236–316. https://doi.org/10.1016/B978-0-12-384747-8.10008-X CrossRefGoogle Scholar
- Haddi K, Jumbo LV, Costa M, Santos M, Faroni L, Serrão J, Oliveira E (2018) Changes in the insecticide susceptibility and physiological trade-offs associated with a host change in the bean weevil Acanthoscelides obtectus. J Pest Sci 91:459–468. https://doi.org/10.1007/s10340-017-0860-1 CrossRefGoogle Scholar
- Korunić Z, Rozman V, Kalinović I (2008) The potential use of natural essential oils in the fumigation of stored agricultural products. In: CAF 2008-Controlled atmosphere and fumigation-green, safe, harmony and developmentGoogle Scholar
- Mwila K, Burton M, Van Dyk J, Pletschke B (2013) The effect of mixtures of organophosphate and carbamate pesticides on acetylcholinesterase and application of chemometrics to identify pesticides in mixtures. Environ Monit Assess 185:2315–2327. https://doi.org/10.1007/s10661-012-2711-0 CrossRefGoogle Scholar
- Neuwinger HD (2000) African traditional medicine: a dictionary of plant use and applications. With supplement: search system for diseases. Teil 2, Medpharm, 589pGoogle Scholar
- Oumarou MK, Younoussa L, Nukenine EN (2018) Toxic effect of Chenopodium ambrosoides, Hyptis suaveolens and Lippia adoensis leaf methanol extracts and essential oils against fourth instar larvae of Anopheles gambiae (Diptera: Culicidae). Int J Mosquito Res 5:61–66Google Scholar
- Sanon A, Garba M, Auger J, Huignard J (2002) Analysis of the insecticidal activity of methylisothiocyanate on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Rondani) (Hymenoptera: Pteromalidae). J Stored Prod Res 38:129–138. https://doi.org/10.1016/s0022-474x(01)00008-x CrossRefGoogle Scholar
- Shou-Min F (2012) Insect glutathione S-transferase: a review of comparative genomic studies and response to xenobiotics. B Insectol 65:265–271Google Scholar
- Waliwitiya R, Nicholson RA, Kennedy CJ, Lowenberger CA (2012) The synergistic effects of insecticidal essential oils and piperonyl butoxide on biotransformational enzyme activities in Aedes aegypti (Diptera: Culicidae). J Med Entomol 49:614–623. https://doi.org/10.1603/ME10272 CrossRefGoogle Scholar
- Wing KD, Schnee ME, Sacher M, Connair M (1998) A novel oxadiazine insecticide is bioactivated in lepidopteran larvae. Arch Insect Biochem Physiol 37:91–103. https://doi.org/10.1002/(SICI)1520-6327(1998)37:13.0 CrossRefGoogle Scholar