Investigation of Selectivity of Amino Functionalised Phases for Pharmaceutical Applications
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Amino column is a special stationary phase with diverse applications on reversed and normal phase, as well as on hydrophilic interaction chromatography. The objective of the present research was to explore the retention mechanism of the amino column, which triggered the detailed study of the chromatographic behavior of 44 drugs (analytes) divided into eight groups. The analytes were studied at five different mobile phases and the retention times were interpreted on the basis of their physicochemical properties and structural features (expressed by 30 variables). The data were fed as input in the partial least squares software and were processed for the development of three different validated models which provided an overview of the behavior of the analytes on amino column. The results showed that the major factors responsible for the chromatographic behavior of the studied drugs on amino column were: the presence of carboxylic group(s), the ionization degree, the pKa values of their basic moieties, and the molar volume. On the contrary, it was observed that the presence of hydroxyl group(s) is indirectly related to the retention phenomenon. In few words, the amino column is attributed a mixed mode depending on the conditions, especially on the composition of the mobile phase. The information derived from such a research could provide a solid base to optimize the use of an amino column in drug analysis not only for research purposes, but also for its use in the pharmaceutical industry.
KeywordsHigh-performance liquid chromatography Amino column Pharmaceutical compounds Partial least squares
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There is no conflict of interest.
Human and Animal Rights
This article does not contain any studies with human participants or animals performed by any of the authors.
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