Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC–MS Applications
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Herein, a zwitterionic organic polymer monolithic stationary phase was prepared based on the in situ thermal-initiated copolymerizing 3-dimethyl-(3-(N-methacrylamido) propyl) ammonium propane sulfonate (DMMPPS) and pentaerythritol triacrylate (PETA) in a binary porogenic solvent consisting of MeOH and H2O. An HILIC/RP dual separation mechanism was observed on this optimised poly(DMMPPS-co-PETA) monolithic column and the composition of the mobile phase corresponding to the transition from the HILIC to the RP mode was around 30% ACN in water. The proposed monolithic column was successfully applied to separate 12 polar neurotransmitters in a nano LC–MS mode. About ~ 27 μm plate height (corresponding to column efficiency of ~ 93,000 plates/m) was obtained at the linear velocity of 1 mm/s. Meantime, the DMMPPS-based monolith exhibited good mechanical stability and excellent separation performance for nucleic acid bases, nucleosides and nucleotides for capillary electrochromatography (CEC). Relative standard deviations (RSD%) of the retention times for five nucleic acid bases and nucleosides were in the range of 0.15–0.42% (run-to-run, n = 3) and 2.63–4.50% (column-to-column, n = 3), respectively. Our work demonstrated that the zwitterionic monolith columns could be an effective separation tool for analysis of hydrophilic substances both on the HI-CEC and nano LC–MS mode.
KeywordsHydrophilic interaction chromatography Capillary electrochromatography Neurotransmitters Zwitterionic monolithic column
This research project was financed by the Xi’an Science and Technology Plan Project (2019KJWL02), the Natural Science Foundation of Shaanxi Province (2018JQ2024), the Special Scientific Research Project of Shaanxi Provincial Education Department (17JK1127) and Shaanxi college students’ innovation and entrepreneurship training project (S201911080054).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 10.Hsiao JJ, Kennedy AP, Van de Bittner GC, Wei T (2018) LC GC 36:30Google Scholar
- 11.Palumbo D, Fais P, Cali A, Lusardi M, Bertol E, Pascali JP (2018) J Chromatogr A 1100:33Google Scholar
- 18.Chen ECY, Wang K, Liu Y, Yang H, Yao S, Chen B, Nie L, Xu G (1877) Electrophoresis 2013:34Google Scholar