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Ultrasound-Assisted Ionic Liquid Solid–Liquid Extraction Coupled with Aqueous Two-Phase Extraction of Naphthoquinone Pigments in Arnebia euchroma (Royle) Johnst.

  • Qianhui Sun
  • Baiquan Du
  • Chenzhao Wang
  • Weili Xu
  • Zhuang Fu
  • Yu Yan
  • Shouzhi Li
  • Zhibing WangEmail author
  • Hanqi Zhang
Original
  • 4 Downloads

Abstract

A simple, rapid and green ultrasound-assisted ionic liquid solid–liquid extraction coupled with aqueous two-phase extraction method was developed and applied to the extraction of shikonin, acetylshikonin and β,β′-dimethylacrylshikonin in Arnebia euchroma (Royle) Johnst. The separation and determination of the analytes was performed by high-performance liquid chromatography coupled with diode array detection. In the study, 1-butyl-3-methylimidazolium tetrafluoroborate ([C4MIM][BF4]) was used as extraction solvent. Ionic liquid-based solid–liquid extraction and aqueous two-phase extraction were performed simultaneously in one tube under the assistance of ultrasound. The liquid–liquid–solid three-phase system, namely surfactant-rich phase, water-rich phase and sample-rich phase, was formed. The target analytes were enriched in the surfactant-rich phase. Under the optimal experimental conditions, the calibration curves of target analytes showed good linear relationship (r > 0.9997). The inter-day and intra-day precision were in the range of 3.42–8.53 and 1.98–3.14%, respectively. The LOD and LOQ for the target analytes were in the range of 5.0–19.2 and 16.7–64.2 ng mL−1, respectively. The recoveries of naphthoquinone pigments ranged from 90.00 to 97.73% and relative standard deviations were less than 4.50%. Compared with heat reflux extraction and ultrasonic-assisted extraction, the proposed method is simpler, faster and more effective, because the mass transfer process for the analytes was always performed in one centrifugal tube and the steps such as filtration, concentration and redissolution were avoided. The present method was demonstrated to be efficient and satisfactory for the extraction of naphthoquinone pigments in medicinal plants.

Graphic Abstract

Keywords

High-performance liquid chromatography Aqueous two-phase extraction Ionic liquid Naphthoquinone pigments Ultrasound-assisted extraction Arnebia euchroma (Royle) Johnst 

Notes

Acknowledgements

This study was financially supported by the Education Department of Jilin Province (Grant no. JJKH20181014KJ) and the Department of Science and Technology of Jilin Province (Grant no. 20190304011YY).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Qianhui Sun
    • 1
  • Baiquan Du
    • 1
  • Chenzhao Wang
    • 1
  • Weili Xu
    • 1
  • Zhuang Fu
    • 1
  • Yu Yan
    • 1
  • Shouzhi Li
    • 1
  • Zhibing Wang
    • 1
    • 2
    Email author
  • Hanqi Zhang
    • 2
  1. 1.College of Chemistry and Life ScienceChangchun University of TechnologyChangchunPeople’s Republic of China
  2. 2.College of ChemistryJilin UniversityChangchunPeople’s Republic of China

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