Ultrasonic Nebulization-Dispersive Solid-Phase-Monolithic Microcolumn Extraction Coupled with HPLC–MS/MS for Analyzing Sex Steroids in Lotion Samples
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An ultrasonic nebulization-dispersive solid-phase-monolithic microcolumn extraction method coupled with high-performance liquid chromatography–mass spectrometry was developed and applied for the extraction and analysis of 15 sex steroids in lotion samples. In this method, the analytes were microextracted from a liquid phase to a dispersive solid phase, and then, a monolithic microcolumn was prepared with these dispersive solid-phase particles using our device. In this way, a more efficient extraction method was developed. Several experimental parameters, including the extraction sorbent, amount of the adsorbent, extraction time, and extraction temperature, were optimized. Calibration curves for steroid concentrations ranging from 0.11 to 1461.00 ng mL−1 were obtained for analyzing the sex steroids. The recoveries in the determination of the sex steroids were between 53.19 and 96.99%, and the relative standard deviations ranged from 2.58 to 7.52%. These results indicated that the proposed method is a fast, sensitive, low-cost, and highly efficient extraction and enrichment technique for the analysis of nonvolatile analytes in liquid samples.
KeywordsHigh-performance liquid chromatography–mass spectrometry Lotion Sex steroids Ultrasonic nebulization-dispersive solid-phase-monolithic microcolumn extraction
This work was supported by the National Natural Science Foundation of China (No. 81401744) and the Natural Science Basic Research Plan in Shaanxi Province of China (Program No. 2017JQ8013).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that there are no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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