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Ultrasonic Nebulization-Dispersive Solid-Phase-Monolithic Microcolumn Extraction Coupled with HPLC–MS/MS for Analyzing Sex Steroids in Lotion Samples

  • Rui ZhangEmail author
Original
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Abstract

An ultrasonic nebulization-dispersive solid-phase-monolithic microcolumn extraction method coupled with high-performance liquid chromatography–mass spectrometry was developed and applied for the extraction and analysis of 15 sex steroids in lotion samples. In this method, the analytes were microextracted from a liquid phase to a dispersive solid phase, and then, a monolithic microcolumn was prepared with these dispersive solid-phase particles using our device. In this way, a more efficient extraction method was developed. Several experimental parameters, including the extraction sorbent, amount of the adsorbent, extraction time, and extraction temperature, were optimized. Calibration curves for steroid concentrations ranging from 0.11 to 1461.00 ng mL−1 were obtained for analyzing the sex steroids. The recoveries in the determination of the sex steroids were between 53.19 and 96.99%, and the relative standard deviations ranged from 2.58 to 7.52%. These results indicated that the proposed method is a fast, sensitive, low-cost, and highly efficient extraction and enrichment technique for the analysis of nonvolatile analytes in liquid samples.

Graphic Abstract

Keywords

High-performance liquid chromatography–mass spectrometry Lotion Sex steroids Ultrasonic nebulization-dispersive solid-phase-monolithic microcolumn extraction 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81401744) and the Natural Science Basic Research Plan in Shaanxi Province of China (Program No. 2017JQ8013).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that there are no conflicts of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical LaboratoryXijing Hospital, Fourth Military Medical UniversityXi’anChina

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