, Volume 82, Issue 10, pp 1571–1577 | Cite as

Simultaneous Determination of Three Herbicides in Honey Samples Using an Aqueous Biphasic System Coupled with HPLC–MS/MS

  • Hongzhe TianEmail author
  • Haoliang Fu
  • Chunqi Xu
  • Chenghao Xu
Short Communication


A simple and environmentally friendly method was developed for simultaneous determination of residue from three triazine herbicides (simetryn, prometryn, and atrazine) from honey samples. The selected herbicides were extracted and purified using an aqueous biphasic system (ABS) based on an ethylene oxide/propylene oxide copolymer (EOPO-2500) and choline acetate ([Ch][Ac]), and determined by HPLC–MS/MS. The partitioning behavior of the analytes using the ABS was investigated, and the analytes mainly concentrated in the copolymer phase. At three spiked levels (2.0, 20.0, 100.0 μg kg−1), the recoveries of the analytes were determined in the range of 81–108%, with relative standard deviations no higher than 10.5%. The limits of quantification for the herbicides ranged from 0.1 to 1.5 µg kg−1. The proposed method is very promising for sample preparation, taking into account its simple operation, mild conditions, and biocompatibility, without the use of any organic solvents during the extraction process.

Graphic abstract


HPLC–MS/MS Aqueous biphasic system Cholinium ionic liquid Triazine herbicide 



This project was financially supported by the National Key Research and Development project of China (grant no. 2017YFD08007001). The authors would like to acknowledge Dr. Robin D. Rogers of The University of Alabama for his support in the research of the aqueous biphasic system.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10337_2019_3781_MOESM1_ESM.pdf (83 kb)
Supplementary material 1 (PDF 83 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Hongzhe Tian
    • 1
    Email author
  • Haoliang Fu
    • 1
  • Chunqi Xu
    • 1
  • Chenghao Xu
    • 1
  1. 1.College of Plant ProtectionShenyang Agricultural UniversityShenyangPeople’s Republic of China

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